MedKoo Cat#: 317998 | Name: Hydrocortisone Acetate
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Hydrocortisone Acetate is the synthetic acetate salt form of hydrocortisone, a corticosteroid with anti-inflammatory and immunosuppressive properties. Hydrocortisone acetate initially binds to the cytoplasmic glucocorticoid receptor; then the receptor-ligand complex is translocated to the nucleus where it initiates the transcription of genes encoding for anti-inflammatory mediators, such as cytokines and lipocortins. Lipocortins inhibit phospholipase A2, thereby blocking the release of arachidonic acid from membrane phospholipids and preventing the synthesis of prostaglandins and leukotrienes.

Chemical Structure

Hydrocortisone Acetate
Hydrocortisone Acetate
CAS#50-03-3 (Acetate)

Theoretical Analysis

MedKoo Cat#: 317998

Name: Hydrocortisone Acetate

CAS#: 50-03-3 (Acetate)

Chemical Formula: C23H32O6

Exact Mass: 404.2199

Molecular Weight: 404.50

Elemental Analysis: C, 68.29; H, 7.97; O, 23.73

Price and Availability

Size Price Availability Quantity
1g USD 250.00 2 Weeks
5g USD 600.00 2 Weeks
10g USD 950.00 2 Weeks
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Synonym
Cortisol 21-acetate; Cortell; 50-03-3; Cortisol acetate; Hydrocortisone 21-acetate;
IUPAC/Chemical Name
2-((8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-10,13-dimethyl-3-oxo-2,3,6,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl)-2-oxoethyl acetate
InChi Key
ALEXXDVDDISNDU-JZYPGELDSA-N
InChi Code
InChI=1S/C23H32O6/c1-13(24)29-12-19(27)23(28)9-7-17-16-5-4-14-10-15(25)6-8-21(14,2)20(16)18(26)11-22(17,23)3/h10,16-18,20,26,28H,4-9,11-12H2,1-3H3/t16-,17-,18-,20+,21-,22-,23-/m0/s1
SMILES Code
CC(OCC([C@@]1(O)CC[C@@H]2[C@]1(C)C[C@H](O)[C@H]3[C@H]2CCC4=CC(CC[C@]34C)=O)=O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Solvent mg/mL mM
Solubility
Soluble in DMSO, not in water 0.0 100.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 404.50 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Ascaso M, Pérez-Lozano P, García M, García-Montoya E, Miñarro M, Ticó JR, Fàbregas A, Carrillo C, Sarrate R, Suñé-Negre JM. Development and Validation of a Stability Indicating RP-HPLC Method for Hydrocortisone Acetate Active Ingredient, Propyl Parahydroxybenzoate and Methyl Parahydroxybenzoate Preservatives, Butylhydroxyanisole Antioxidant, and Their Degradation Products in a Rectal Gel Formulation. J AOAC Int. 2015 Jan-Feb;98(1):27-34. doi: 10.5740/jaoacint.13-411. PubMed PMID: 25857875. 2: Dołowy M, Kulpińska-Kucia K, Pyka A. Validation of a thin-layer chromatography for the determination of hydrocortisone acetate and lidocaine in a pharmaceutical preparation. ScientificWorldJournal. 2014 Jan 6;2014:107879. doi: 10.1155/2014/107879. eCollection 2014. PubMed PMID: 24526880; PubMed Central PMCID: PMC3913387. 3: Korany MA, Maher HM, Galal SM, Ragab MA. Development and optimization of a capillary zone electrophoresis technique for simultaneous determination of miconazole nitrate and hydrocortisone acetate in a cream pharmaceutical formulation. J AOAC Int. 2013 Nov-Dec;96(6):1295-301. PubMed PMID: 24645507. 4: Canpolat F, Erkoçoğlu M, Tezer H, Kocabaş CN, Kandi B. Hydrocortisone acetate alone or combined with mupirocin for atopic dermatitis in infants under two years of age - a randomized double blind pilot trial. Eur Rev Med Pharmacol Sci. 2012 Dec;16(14):1989-93. PubMed PMID: 23242727. 5: Lee MS, Seo SR, Kim JC. A β-cyclodextrin, polyethyleneimine and silk fibroin hydrogel containing Centella asiatica extract and hydrocortisone acetate: releasing properties and in vivo efficacy for healing of pressure sores. Clin Exp Dermatol. 2012 Oct;37(7):762-71. doi: 10.1111/j.1365-2230.2011.04331.x. Epub 2012 Mar 22. PubMed PMID: 22439751. 6: Gurney AB, Wascher D, Schenck R, Tennison A, Jaramillo B. Absorption of hydrocortisone acetate in human connective tissue using phonophoresis. Sports Health. 2011 Jul;3(4):346-51. PubMed PMID: 23016027; PubMed Central PMCID: PMC3445200. 7: Kircik LH. Efficacy and onset of action of hydrocortisone acetate 2.5% and pramoxine hydrochloride 1% lotion for the management of pruritus: results of a pilot study. J Clin Aesthet Dermatol. 2011 Feb;4(2):48-50. PubMed PMID: 21386957; PubMed Central PMCID: PMC3050616. 8: Singh M, Niranjan HS, Mehrotra R, Sharma D, Gupta SC. Efficacy of hydrocortisone acetate/hyaluronidase vs triamcinolone acetonide/hyaluronidase in the treatment of oral submucous fibrosis. Indian J Med Res. 2010 May;131:665-9. PubMed PMID: 20516538. 9: Jancic-Stojanović B, Malenović A, Marković S, Ivanović D, Medenica M. Optimization and validation of an RP-HPLC method for analysis of hydrocortisone acetate and lidocaine in suppositories. J AOAC Int. 2010 Jan-Feb;93(1):102-7. PubMed PMID: 20334171. 10: Burnett BP, Mitchell CM. Antimicrobial activity of iodoquinol 1%-hydrocortisone acetate 2% gel against ciclopirox and clotrimazole. Cutis. 2008 Oct;82(4):273-80. PubMed PMID: 19055171. 11: Fini A, Bergamante V, Ceschel GC, Ronchi C, De Moraes CA. Control of transdermal permeation of hydrocortisone acetate from hydrophilic and lipophilic formulations. AAPS PharmSciTech. 2008;9(3):762-8. doi: 10.1208/s12249-008-9107-z. Epub 2008 Jun 18. Review. PubMed PMID: 18563577; PubMed Central PMCID: PMC2977040. 12: Firooz A, Solhpour A, Gorouhi F, Daneshpazhooh M, Balighi K, Farsinejad K, Rashighi-Firoozabadi M, Dowlati Y. Pimecrolimus cream, 1%, vs hydrocortisone acetate cream, 1%, in the treatment of facial seborrheic dermatitis: a randomized, investigator-blind, clinical trial. Arch Dermatol. 2006 Aug;142(8):1066-7. PubMed PMID: 16924062. 13: Reitamo S, Harper J, Bos JD, Cambazard F, Bruijnzeel-Koomen C, Valk P, Smith C, Moss C, Dobozy A, Palatsi R; European Tacrolimus Ointment Group. 0.03% Tacrolimus ointment applied once or twice daily is more efficacious than 1% hydrocortisone acetate in children with moderate to severe atopic dermatitis: results of a randomized double-blind controlled trial. Br J Dermatol. 2004 Mar;150(3):554-62. PubMed PMID: 15030341. 14: Raghavan SL, Kiepfer B, Davis AF, Kazarian SG, Hadgraft J. Membrane transport of hydrocortisone acetate from supersaturated solutions; the role of polymers. Int J Pharm. 2001 Jun 19;221(1-2):95-105. PubMed PMID: 11397571. 15: Raghavan SL, Trividic A, Davis AF, Hadgraft J. Crystallization of hydrocortisone acetate: influence of polymers. Int J Pharm. 2001 Jan 16;212(2):213-21. PubMed PMID: 11165079. 16: Bilgihan K, Ozdek S, Ozoğul C, Gurelik G, Bilgihan A, Hasanreisoğlu B. Topical vitamin E and hydrocortisone acetate treatment after photorefractive keratectomy. Eye (Lond). 2000 Apr;14 ( Pt 2):231-7. PubMed PMID: 10845023. 17: Blanco M, Coello J, Iturriaga H, Maspoch S, Villegas N. Kinetic spectrophotometric determination of hydrocortisone acetate in a pharmaceutical preparation by use of partial least-squares regression. Analyst. 1999 Jun;124(6):911-5. PubMed PMID: 10736875. 18: Harris PD, Soleng A, Bakke TA. Increased susceptibility of salmonids to the monogenean Gyrodactylus salaris following administration of hydrocortisone acetate. Parasitology. 2000 Jan;120 ( Pt 1):57-64. PubMed PMID: 10726266. 19: Raghavan SL, Trividic A, Davis AF, Hadgraft J. Effect of cellulose polymers on supersaturation and in vitro membrane transport of hydrocortisone acetate. Int J Pharm. 2000 Jan 5;193(2):231-7. PubMed PMID: 10606787. 20: Tagashira Y, Kitajima A, Maekawa K, Yoshii K, Tanimoto T, Okada S. [Hydrocortisone Acetate Reference Standard (Control 961) of National Institute of Health Sciences]. Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku. 1997;(115):196-8. Japanese. PubMed PMID: 9641843.