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MedKoo Cat#: 563431 | Name: Muscimol HBr
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Muscimol HBr is a GABAA receptor agonist.

Chemical Structure

Muscimol HBr
Muscimol HBr
CAS#18174-72-6 (HBr)

Theoretical Analysis

MedKoo Cat#: 563431

Name: Muscimol HBr

CAS#: 18174-72-6 (HBr)

Chemical Formula: C4H7BrN2O2

Exact Mass: 0.0000

Molecular Weight: 195.02

Elemental Analysis: C, 24.64; H, 3.62; Br, 40.97; N, 14.36; O, 16.41

Price and Availability

Size Price Availability Quantity
5mg USD 290.00
10mg USD 470.00
25mg USD 820.00
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Related CAS #
2763-96-4 (free base) 18174-72-6 (HBr) 3579-03-1 (HCl)
Synonym
Muscimol Hydrobromide; Muscimol HBr; Agarin hydrobromide; Agarin HBr;
IUPAC/Chemical Name
5-(aminomethyl)isoxazol-3(2H)-one hydrobromide
InChi Key
OPZOJWHOZRKYQX-UHFFFAOYSA-N
InChi Code
InChI=1S/C4H6N2O2.BrH/c5-2-3-1-4(7)6-8-3;/h1H,2,5H2,(H,6,7);1H
SMILES Code
O=C1NOC(CN)=C1.[H]Br
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Muscimol HBr is a GABAA receptor agonist.
In vitro activity:
The present study was performed to identify the effect of GABAR agonists on K2P channel expression and activity in the neuroblastic B35 cells that maintain glutamic acid decarboxylase (GAD) activity and express GABA. In particular, muscimol, a GABAAR agonist, significantly increased TREK-2 expression and activity, but the effect was reduced in the presence of the GABAAR antagonist bicuculine or TREK-2 inhibitor norfluoxetine. In the whole-cell and single-channel patch configurations, muscimol increased TREK-2 activity, but the muscimol effect disappeared in the N-terminal deletion mutant. Reference: Int J Mol Sci. 2021 Aug 27;22(17):9320. https://pubmed.ncbi.nlm.nih.gov/34502229/
In vivo activity:
This study hypothesized that muscimol, a selective GABA(A) agonist, could act on endogenous GABA(A) channels expressed on airway smooth muscle to attenuate induced increases in airway pressures in anesthetized guinea pigs in vivo. Pretreatment with intravenous muscimol alone attenuated intravenous histamine-, intravenous acetylcholine-, or vagal nerve-stimulated increases in peak pulmonary inflation pressure. Pretreatment with the GABA(A) antagonist gabazine blocked muscimol's effect. Reference: J Appl Physiol (1985). 2009 Apr;106(4):1257-63. https://pubmed.ncbi.nlm.nih.gov/19213928/

Preparing Stock Solutions

The following data is based on the product molecular weight 195.02 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Kim EJ, Kwon OS, Hur CG, Nyiramana MM, Lee DK, Hong SG, Han J, Kang D. Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus. Int J Mol Sci. 2021 Aug 27;22(17):9320. doi: 10.3390/ijms22179320. PMID: 34502229; PMCID: PMC8431218. 2. Kanasaki H, Tumurbaatar T, Oride A, Hara T, Okada H, Kyo S. Gamma-aminobutyric acidA receptor agonist, muscimol, increases KiSS-1 gene expression in hypothalamic cell models. Reprod Med Biol. 2017 Oct 4;16(4):386-391. doi: 10.1002/rmb2.12061. PMID: 29259493; PMCID: PMC5715903. 3. Jafari-Sabet M, Jannat-Dastjerdi I. Muscimol state-dependent memory: involvement of dorsal hippocampal mu-opioid receptors. Behav Brain Res. 2009 Aug 24;202(1):5-10. doi: 10.1016/j.bbr.2009.03.010. Epub 2009 Mar 19. PMID: 19447274. 4. Gleason NR, Gallos G, Zhang Y, Emala CW. The GABAA agonist muscimol attenuates induced airway constriction in guinea pigs in vivo. J Appl Physiol (1985). 2009 Apr;106(4):1257-63. doi: 10.1152/japplphysiol.91314.2008. Epub 2009 Feb 12. PMID: 19213928; PMCID: PMC2698643.
In vitro protocol:
1. Kim EJ, Kwon OS, Hur CG, Nyiramana MM, Lee DK, Hong SG, Han J, Kang D. Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus. Int J Mol Sci. 2021 Aug 27;22(17):9320. doi: 10.3390/ijms22179320. PMID: 34502229; PMCID: PMC8431218. 2. Kanasaki H, Tumurbaatar T, Oride A, Hara T, Okada H, Kyo S. Gamma-aminobutyric acidA receptor agonist, muscimol, increases KiSS-1 gene expression in hypothalamic cell models. Reprod Med Biol. 2017 Oct 4;16(4):386-391. doi: 10.1002/rmb2.12061. PMID: 29259493; PMCID: PMC5715903.
In vivo protocol:
1. Jafari-Sabet M, Jannat-Dastjerdi I. Muscimol state-dependent memory: involvement of dorsal hippocampal mu-opioid receptors. Behav Brain Res. 2009 Aug 24;202(1):5-10. doi: 10.1016/j.bbr.2009.03.010. Epub 2009 Mar 19. PMID: 19447274. 2. Gleason NR, Gallos G, Zhang Y, Emala CW. The GABAA agonist muscimol attenuates induced airway constriction in guinea pigs in vivo. J Appl Physiol (1985). 2009 Apr;106(4):1257-63. doi: 10.1152/japplphysiol.91314.2008. Epub 2009 Feb 12. PMID: 19213928; PMCID: PMC2698643.
1: Jang IS, Nakamura M, Ito Y, Akaike N. Presynaptic GABAA receptors facilitate spontaneous glutamate release from presynaptic terminals on mechanically dissociated rat CA3 pyramidal neurons. Neuroscience. 2006;138(1):25-35. Epub 2005 Dec 27. PubMed PMID: 16378694.