EGS Crosslinker | CAS# 70539-42-3 | Crosslinker

MedKoo Cat#: 571625 | Name: EGS Crosslinker

Description:

WARNING: This product is for research use only, not for human or veterinary use.

EGS Crosslinker, or Ethylene glycol bis(succinimidylsuccinate), is a water insoluble homobifunctional crosslinker that can be used to label intracellular proteins. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions.

Chemical Structure

EGS Crosslinker

CAS#70539-42-3

Theoretical Analysis

MedKoo Cat#: 571625

Name: EGS Crosslinker

CAS#: 70539-42-3

Chemical Formula: C18H20N2O12

Exact Mass: 456.1016

Molecular Weight: 456.36

Elemental Analysis: C, 47.37; H, 4.42; N, 6.14; O, 42.07

Price and Availability

Size	Price	Availability
50mg	USD 150.00	Ready to ship
100mg	USD 250.00	Ready to ship
200mg	USD 350.00	Ready to ship
500mg	USD 450.00	Ready to ship
1g	USD 550.00	Ready to ship
2g	USD 850.00	Ready to ship
5g	USD 1,250.00	Ready to ship

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Related CAS #

167410-92-6 (sulfo) 70539-42-3

Synonym

EGS Crosslinker, EGS Cross linker

IUPAC/Chemical Name

Ethylene Glycol-Bis (Succinic Acid N-Hydroxysuccinimide Ester)

InChi Key

QLHLYJHNOCILIT-UHFFFAOYSA-N

InChi Code

InChI=1S/C18H20N2O12/c21-11-1-2-12(22)19(11)31-17(27)7-5-15(25)29-9-10-30-16(26)6-8-18(28)32-20-13(23)3-4-14(20)24/h1-10H2

SMILES Code

O=C(OCCOC(CCC(ON1C(CCC1=O)=O)=O)=O)CCC(ON2C(CCC2=O)=O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

EGS Crosslinker must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1 angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable. EGS crosslinker's spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule.

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#A21T11K01

QC Data

View QC data: current batch, Lot#A21T11K01

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

EGS Crosslinker (EGNHS) is an alkyl/ether-based PROTAC linker that can be used in the synthesis of PROTACs.

In vitro activity:

The bifunctional compound, ethylene-glycol bis(N-hydroxysuccinimidylsuccinate) (EGNHS), stabilizes horseradish peroxidase C (HRP) by reaction with the enzyme's lysine residues. Most significantly, EGNHS is shown to form a crosslink between Lys232 and Lys241 of HRP and modifies Lys174 without formation of a crosslink. These findings are in agreement with the lysine side-chain reactivities predicted from the surface accessibility of the amino groups, and the maximal span of 16 A of the EGNHS crosslinker. Reference: Biotechnol Bioeng. 2001 Dec;76(4):277-84. https://pubmed.ncbi.nlm.nih.gov/11745154/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMSO	100.0	219.13

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 456.36 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. O'Brien AM, O'Fágáin C, Nielsen PF, Welinder KG. Location of crosslinks in chemically stabilized horseradish peroxidase: implications for design of crosslinks. Biotechnol Bioeng. 2001 Dec;76(4):277-84. doi: 10.1002/bit.1194. PMID: 11745154.

In vitro protocol:

In vivo protocol:

TBD

1: Dreiling A, Hanneken M, König S. Early cleavage of ethylene glycol bis(succinimidylsuccinate) (EGS)-linker moieties during enzymatic digestion of cross-linked proteins. Rapid Commun Mass Spectrom. 2014 Nov 15;28(21):2385-8. doi: 10.1002/rcm.7034. PubMed PMID: 25279753. 2: Lakra R, Kiran MS, Sai KP. Fabrication of homobifunctional crosslinker stabilized collagen for biomedical application. Biomed Mater. 2015 Nov 27;10(6):065015. doi: 10.1088/1748-6041/10/6/065015. PubMed PMID: 26610606.