EDA-DA is an unnatural dipeptide building block (an ethynyl-D-alanine and a D-alanine). It incorporates a biorthogonal alkyne group into peptidoglycan (PG) through MurF in the cytoplasmic pathway, which enables selective labeling via a click-chemistry reaction. EDA-DA allows labeling of PG in Gram-positive (B. subtilis), Gram-negative (E. coli and C. trachomatis), Mycobacterium (M. smegmatis) and moss plastids (P. patens) with azide modified fluorescent dyes such as Alexa Fluor 488.
MedKoo Cat#: 466255
Name: EDA-DA TFA salt
CAS#: 87156-01-2
Chemical Formula: C10H13F3N2O5
Exact Mass: 298.0777
Molecular Weight: 298.22
Elemental Analysis: C, 40.28; H, 4.39; F, 19.11; N, 9.39; O, 26.82
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 29.8 | 100.00 | |
| Water | 29.8 | 100.00 |
The following data is based on the product molecular weight 298.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
