Synonym
EDC-HCl Crosslinker; EDC hydrochloride; EDC HCl; EDAC·HCl; EDCI·HCl;
IUPAC/Chemical Name
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide HCl
InChi Key
FPQQSJJWHUJYPU-UHFFFAOYSA-N
InChi Code
InChI=1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
SMILES Code
CCN=C=NCCCN(C)C.[H]Cl
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
EDC-HCl is water soluble and reactions can take place at physiological conditions. The best results take place at pH of 5.5 or less when buffered in MES. Sulfo-NHS or NHS is used as a catalyst for EDC-HCl coupling and greatly increases the efficiency of the reaction.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
191.70
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
4: Krishnamoorthy G, Selvakumar R, Sastry TP, Sadulla S, Mandal AB, Doble M. Experimental and theoretical studies on Gallic acid assisted EDC/NHS initiated crosslinked collagen scaffolds. Mater Sci Eng C Mater Biol Appl. 2014 Oct;43:164-71. doi: 10.1016/j.msec.2014.07.003. Epub 2014 Jul 8. PubMed PMID: 25175201.
5: Zhao P, Deng C, Xu H, Tang X, He H, Lin C, Su J. Fabrication of photo-crosslinked chitosan- gelatin scaffold in sodium alginate hydrogel for chondrocyte culture. Biomed Mater Eng. 2014;24(1):633-41. doi: 10.3233/BME-130851. PubMed PMID: 24211948.
6: Li W, Long Y, Liu Y, Long K, Liu S, Wang Z, Wang Y, Ren L. Fabrication and characterization of chitosan-collagen crosslinked membranes for corneal tissue engineering. J Biomater Sci Polym Ed. 2014;25(17):1962-72. doi: 10.1080/09205063.2014.965996. Epub 2014 Oct 9. PubMed PMID: 25299624.
7: Staroszczyk H, Sztuka K, Wolska J, Wojtasz-Pająk A, Kołodziejska I. Interactions of fish gelatin and chitosan in uncrosslinked and crosslinked with EDC films: FT-IR study. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jan 3;117:707-12. doi: 10.1016/j.saa.2013.09.044. Epub 2013 Sep 18. PubMed PMID: 24140742.
