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MedKoo Cat#: 414500 | Name: Homidium Free Base

Description:

WARNING: This product is for research use only, not for human or veterinary use.

Homidium Free Base is a trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.

Chemical Structure

Homidium Free Base
Homidium Free Base
CAS#3546-21-2 (free base)

Theoretical Analysis

MedKoo Cat#: 414500

Name: Homidium Free Base

CAS#: 3546-21-2 (free base)

Chemical Formula: C21H20N3+

Exact Mass: 314.1652

Molecular Weight: 314.41

Elemental Analysis: C, 80.22; H, 6.41; N, 13.36

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.
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Related CAS #
3546-21-2 (free base) 602-52-8 (Cl) 1239-45-8 (Br)
Synonym
Homidium Free Base; BRN3627183; BRN-3627183 BRN 3627183; Homidium; Novidium
IUPAC/Chemical Name
Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-
InChi Key
QTANTQQOYSUMLC-UHFFFAOYSA-O
InChi Code
InChI=1S/C21H19N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,23H,2,22H2,1H3/p+1
SMILES Code
CC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 314.41 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Dolan RB, Okech G, Alushula H, Mutugi M, Stevenson P, Sayer PD, Njogu AR. Homidium bromide as a chemoprophylactic for cattle trypanosomiasis in Kenya. Acta Trop. 1990 Mar;47(3):137-44. doi: 10.1016/0001-706x(90)90019-v. PMID: 1971490. 2: Petrov A, Wu T, Puglisi EV, Puglisi JD. RNA purification by preparative polyacrylamide gel electrophoresis. Methods Enzymol. 2013;530:315-30. doi: 10.1016/B978-0-12-420037-1.00017-8. PMID: 24034329. 3: Murilla GA, Mdachi RE, Ismail AA, Karanja WM. Bioavailability, pharmacokinetics, and tissue distribution of 14C homidium after parenteral administration to Boran cattle. J Vet Pharmacol Ther. 1996 Apr;19(2):142-8. doi: 10.1111/j.1365-2885.1996.tb00027.x. PMID: 8735423. 4: Murilla GA, Eisler MC, Peregrine AS, Ndung'u JM, Holmes PH. Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle. J Vet Pharmacol Ther. 1999 Oct;22(5):301-7. doi: 10.1046/j.1365-2885.1999.00216.x. PMID: 10597533. 5: Huang Q, Fu WL. Comparative analysis of the DNA staining efficiencies of different fluorescent dyes in preparative agarose gel electrophoresis. Clin Chem Lab Med. 2005;43(8):841-2. doi: 10.1515/CCLM.2005.141. PMID: 16201894. 6: Kibugu JK, Muchiri MW, Ngae GN, Mumba AM, Kiragu JM. Effects of Ethidium (homidium bromide) on female reproductive performance of laboratory-reared tsetse flies, Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Acta Trop. 2010 Nov;116(2):157-60. doi: 10.1016/j.actatropica.2010.07.007. Epub 2010 Aug 1. PMID: 20678462. 7: Stevenson P, Sones KR, Gicheru MM, Mwangi EK. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. doi: 10.1016/0001-706x(94)00080-k. PMID: 7676909. 8: Murilla GA, Peregrine AS, Ndung'u JM, Holmes PH, Eisler MC. The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle. Acta Trop. 2002 Mar;81(3):185-95. doi: 10.1016/s0001-706x(01)00209-1. PMID: 11835895. 9: Murilla GA, Holmes PH, Peregrine AS, Eisler MC, Ndung'u JM. Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA). J Vet Pharmacol Ther. 1999 Oct;22(5):295-300. doi: 10.1046/j.1365-2885.1999.00215.x. PMID: 10597532. 10: Geerts S, De Deken R, Kageruka P, Lootens K, Schacht E. Evaluation of the efficacy of a slow release device containing homidium bromide in rabbits infected with Trypanosoma congolense. Vet Parasitol. 1993 Oct;50(1-2):15-21. doi: 10.1016/0304-4017(93)90003-6. PMID: 8291190.