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MedKoo Cat#: 463173 | Name: Timosaponin BII
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Timosaponin BII (TB-II) is a steroidal saponin that has been found in Anemarrhenae and has diverse biological activities. TB-II inhibits superoxide generation induced by phorbol 12-myristate 13-acetate, N-formyl-Met-Leu-Phe, or arachidonic acid in isolated human neutrophils. It reduces cell death induced by enterovirus 71 in Vero cells. TB-II inhibits ADP-induced platelet aggregation in isolated rabbit platelet-rich plasma (PRP). TB-II increases activated partial thromboplastin time (APTT) and decreases the wet weight and length of thrombi in Chandler’s ex vivo thrombosis model.

Chemical Structure

Timosaponin BII
CAS#136656-07-0

Theoretical Analysis

MedKoo Cat#: 463173

Name: Timosaponin BII

CAS#: 136656-07-0

Chemical Formula: C45H76O19

Exact Mass: 920.4981

Molecular Weight: 921.08

Elemental Analysis: C, 58.68; H, 8.32; O, 33.00

Price and Availability

Size Price Availability Quantity
1mg USD 350.00 2 Weeks
5mg USD 750.00 2 Weeks
10mg USD 1,250.00 2 Weeks
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Synonym
Timosaponin BII; TB-II;
IUPAC/Chemical Name
(2S,3R,4S,5S,6R)-2-(((2R,3R,4S,5R,6R)-4,5-dihydroxy-2-(((2aR,4S,6aS,6bS,8aS,8bR,9S,10R,11aS,12aS,12bR)-10-hydroxy-6a,8a,9-trimethyl-10-((S)-3-methyl-4-(((2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)butyl)octadecahydro-1H-naphtho[2',1':4,5]indeno[2,1-b]furan-4-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
InChi Key
SORUXVRKWOHYEO-UJDJZYEZSA-N
InChi Code
InChI=1S/C45H76O19/c1-19(18-58-40-37(55)34(52)31(49)27(15-46)60-40)7-12-45(57)20(2)30-26(64-45)14-25-23-6-5-21-13-22(8-10-43(21,3)24(23)9-11-44(25,30)4)59-42-39(36(54)33(51)29(17-48)62-42)63-41-38(56)35(53)32(50)28(16-47)61-41/h19-42,46-57H,5-18H2,1-4H3/t19-,20-,21+,22-,23+,24-,25-,26-,27+,28+,29+,30-,31+,32+,33-,34-,35-,36-,37+,38+,39+,40+,41-,42+,43-,44-,45+/m0/s1
SMILES Code
C[C@@H](CC[C@]1(O[C@]2(C[C@]3([C@@]4(CC[C@@]5(C[C@H](CC[C@@]5([C@]4(CC[C@@]3([C@]2([C@@H]1C)[H])C)[H])C)O[C@H]6[C@@H]([C@H]([C@H]([C@H](O6)CO)O)O)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)CO)O)O)O)[H])[H])[H])[H])O)CO[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O)O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 921.08 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Xiao L, Zhang X, Chen Z, Li J, Li B, Li L. Molecular Pathways Involved in Promoting Activity of Timosaponin BII on Hair Growth in C57BL/6 Mice. Biomed Res Int. 2020 Sep 18;2020:9451596. doi: 10.1155/2020/9451596. PMID: 33015188; PMCID: PMC7520677. 2: Xiao L, Zhang X, Chen Z, Li B, Li L. A Timosaponin B-II containing scalp care solution for improvement of scalp hydration, dandruff reduction and hair loss prevention: A comparative study on healthy volunteers before and after application. J Cosmet Dermatol. 2020 Aug 11. doi: 10.1111/jocd.13672. Epub ahead of print. PMID: 32780920. 3: Xie L, Lee DY, Shang Y, Cao X, Wang S, Liao J, Zhang T, Dai R. Characterization of spirostanol glycosides and furostanol glycosides from anemarrhenae rhizoma as dual targeted inhibitors of 5-lipoxygenase and Cyclooxygenase-2 by employing a combination of affinity ultrafiltration and HPLC/MS. Phytomedicine. 2020 Jul 11;77:153284. doi: 10.1016/j.phymed.2020.153284. Epub ahead of print. PMID: 32707371. 4: Du MJ, Chen JP, Lian BW, Mei WJ, Yu JX, Chen LS, Deng SB, Zhong YM, Yu CQ. Cardioprotective effects of timosaponin B-II isolated from Anemarrhena rhizome in a zebrafish model. Pharmazie. 2020 May 1;75(5):201-204. doi: 10.1691/ph.2020.9869. PMID: 32393429. 5: Tang XY, Gao MX, Xiao HH, Yun WJ, Dai Y, Yao ZH, Wong MS, Yao XS. Simultaneous Quantitative Analysis of Multiple Biotransformation Products of Xian-Ling-Gu-Bao, a Traditional Chinese Medicine Prescription, with Rat Intestinal Microflora by Ultra-Performance Liquid Chromatography Tandem Triple Quadrupole Mass Spectrometry. J Chromatogr Sci. 2020 Jun 5;58(6):494-503. doi: 10.1093/chromsci/bmaa012. PMID: 32236407. 6: Shi K, Zhu J, Chen D, Ren C, Guo M, Wang J, Wu X, Feng Y. Lipidomics Analysis of Timosaponin BII in INS-1 Cells Induced by Glycolipid Toxicity and Its Relationship with Inflammation. Chem Biodivers. 2020 Apr;17(4):e1900684. doi: 10.1002/cbdv.201900684. Epub 2020 Apr 3. PMID: 32064755. 7: Chen W, Li R, Zhu S, Ma J, Pang L, Ma B, Du L, Jin Y. Nasal timosaponin BII dually sensitive in situ hydrogels for the prevention of Alzheimer's disease induced by lipopolysaccharides. Int J Pharm. 2020 Mar 30;578:119115. doi: 10.1016/j.ijpharm.2020.119115. Epub 2020 Feb 8. PMID: 32045690. 8: Zhong M, Tian X, Chen S, Chen M, Guo Z, Zhang M, Zheng G, Li Z, Shi Z, Wang G, Gao H, Liu F, Huang C. Identifying the active components of Baihe-Zhimu decoction that ameliorate depressive disease by an effective integrated strategy: a systemic pharmacokinetics study combined with classical depression model tests. Chin Med. 2019 Sep 24;14:37. doi: 10.1186/s13020-019-0254-9. Erratum in: Chin Med. 2020 Apr 2;15:32. PMID: 31572489; PMCID: PMC6757420. 9: Li H, Wu C, Liu Y, Zhang S, Gao X. Baihe Zhimu formula attenuates the efficacy of tamoxifen against breast cancer in mice through modulation of CYP450 enzymes. BMC Complement Altern Med. 2019 Sep 4;19(1):240. doi: 10.1186/s12906-019-2651-0. PMID: 31484532; PMCID: PMC6727345. 10: Tang XY, Dai ZQ, Wu QC, Zeng JX, Gao MX, Xiao HH, Yao ZH, Dai Y, Yao XS. Simultaneous determination of multiple components in rat plasma and pharmacokinetic studies at a pharmacodynamic dose of Xian-Ling-Gu-Bao capsule by UPLC-MS/MS. J Pharm Biomed Anal. 2020 Jan 5;177:112836. doi: 10.1016/j.jpba.2019.112836. Epub 2019 Aug 26. PMID: 31473481. 11: Wang N, Xu P, Wang X, Yao W, Yu Z, Wu R, Huang X, Si Y, Shou D. Integrated pathological cell fishing and network pharmacology approach to investigate main active components of Er-Xian decotion for treating osteoporosis. J Ethnopharmacol. 2019 Sep 15;241:111977. doi: 10.1016/j.jep.2019.111977. Epub 2019 May 25. PMID: 31136804. 12: Ji KY, Kim KM, Kim YH, Im AR, Lee JY, Park B, Na M, Chae S. The enhancing immune response and anti-inflammatory effects of Anemarrhena asphodeloides extract in RAW 264.7 cells. Phytomedicine. 2019 Jun;59:152789. doi: 10.1016/j.phymed.2018.12.012. Epub 2018 Dec 13. PMID: 31009851. 13: Xie Q, Zhao H, Li N, Su L, Xu X, Hong Z. Protective effects of timosaponin BII on oxidative stress damage in PC12 cells based on metabolomics. Biomed Chromatogr. 2018 Oct;32(10):e4321. doi: 10.1002/bmc.4321. Epub 2018 Jul 4. PMID: 29920723. 14: Ji D, Su X, Huang Z, Wang Q, Lu T. A novel ultra high-performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of xanthones and steroidal saponins in crude and salt-processed Anemarrhenae Rhizoma aqueous extracts. J Sep Sci. 2018 Jun;41(11):2310-2320. doi: 10.1002/jssc.201701408. Epub 2018 Mar 15. PMID: 29473301. 15: Yang B, Liu Z, Wang Q, Chai Y, Xia P. Pharmacokinetic comparison of seven major bioactive components in normal and depression model rats after oral administration of Baihe Zhimu decoction by liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal. 2018 Jan 30;148:119-127. doi: 10.1016/j.jpba.2017.09.031. Epub 2017 Sep 28. PMID: 28987996. 16: Lin N, Liu B, Zhang J, Long Y, Dong G, Jin H, Ma B. Acute toxicity, 28-day repeated-dose toxicity and toxicokinetic study of timosaponin BII in rats. Regul Toxicol Pharmacol. 2017 Nov;90:244-257. doi: 10.1016/j.yrtph.2017.09.021. Epub 2017 Sep 22. PMID: 28947379. 17: Wang N, Zhang Q, Xin H, Shou D, Qin L. Osteoblast cell membrane chromatography coupled with liquid chromatography and time-of-flight mass spectrometry for screening specific active components from traditional Chinese medicines. J Sep Sci. 2017 Nov;40(22):4311-4319. doi: 10.1002/jssc.201700688. Epub 2017 Oct 6. PMID: 28884956. 18: Nian SH, Li HJ, Liu EH, Li P. Comparison of α-glucosidase inhibitory effect and bioactive constituents of Anemarrhenae Rhizoma and Fibrous Roots. J Pharm Biomed Anal. 2017 Oct 25;145:195-202. doi: 10.1016/j.jpba.2017.06.039. Epub 2017 Jun 19. PMID: 28666166. 19: Yao ZH, Qin ZF, Cheng H, Wu XM, Dai Y, Wang XL, Qin L, Ye WC, Yao XS. Simultaneous Quantification of Multiple Representative Components in the Xian- Ling-Gu-Bao Capsule by Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry. Molecules. 2017 Jun 2;22(6):927. doi: 10.3390/molecules22060927. PMID: 28574448; PMCID: PMC6152775. 20: Zhu L, Wang Z, Zhai X, Sui Z, Wang D, Li Q, Bi K, He B, Wang T. Simultaneous quantitative determination of 13 active components in the traditional Chinese medicinal preparation Suanzaoren oral liquid by HPLC coupled with diode array detection and evaporative light scattering detection. J Sep Sci. 2017 Jun;40(11):2320-2325. doi: 10.1002/jssc.201700061. Epub 2017 Apr 24. PMID: 28371233.