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MedKoo Cat#: 562949 | Name: Resazurin sodium
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Resazurin is an oxidation-reduction indicator in cell viability. Resazurin is a blue, non-fluorescent dye originally used to test milk/biological fluids for bacterial and yeast contamination. It is also useful in sperm viability and semen quality test. Resazurin is applicable in cytotoxicity determination. Resazurin sodium salt has been used in viability assay: such as to measure cell viability of A. fumigatus; to assess cell proliferation; and to determine A. fumigatus viability in high-throughput screenings of microbial natural extracts.

Chemical Structure

Resazurin sodium
Resazurin sodium
CAS#62758-13-8 (sodium)

Theoretical Analysis

MedKoo Cat#: 562949

Name: Resazurin sodium

CAS#: 62758-13-8 (sodium)

Chemical Formula: C12H6NNaO4

Exact Mass: 229.0375

Molecular Weight: 251.17

Elemental Analysis: C, 57.38; H, 2.41; N, 5.58; Na, 9.15; O, 25.48

Price and Availability

Size Price Availability Quantity
1g USD 250.00 2 Weeks
5g USD 550.00 2 Weeks
10g USD 750.00 2 Weeks
25g USD 1,250.00 2 Weeks
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Related CAS #
62758-13-8 (sodium) 550-82-3 (free)
Synonym
Resazurin sodium; Resazurin sodium salt; Resazurin; Diazoresorcinol; Azoresorcin; Resazoin; Resazurine;
IUPAC/Chemical Name
7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt
InChi Key
IVGPGQSSDLDOLH-UHFFFAOYSA-M
InChi Code
InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
SMILES Code
O=C1C=CC2=[N+]([O-])C3=C(C=C([O-])C=C3)OC2=C1.[Na+]
Appearance
Solid powder
Purity
Dye content, ~90%
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 251.17 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Lin S, Yang L, Chen G, Li B, Chen D, Li L, Xu Z. Pathogenic features and characteristics of food borne pathogens biofilm: Biomass, viability and matrix. Microb Pathog. 2017 Oct;111:285-291. doi: 10.1016/j.micpath.2017.08.005. Epub 2017 Aug 9. Review. PubMed PMID: 28803003. 2: Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, Minor L. Cell Viability Assays. 2013 May 1 [updated 2016 Jul 1]. In: Sittampalam GS, Coussens NP, Brimacombe K, Grossman A, Arkin M, Auld D, Austin C, Baell J, Bejcek B, Chung TDY, Dahlin JL, Devanaryan V, Foley TL, Glicksman M, Hall MD, Hass JV, Inglese J, Iversen PW, Kahl SD, Kales SC, Lal-Nag M, Li Z, McGee J, McManus O, Riss T, Trask OJ Jr., Weidner JR, Xia M, Xu X, editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Available from http://www.ncbi.nlm.nih.gov/books/NBK144065/ PubMed PMID: 23805433. 3: Hall MD, Simeonov A, Davis MI. Avoiding Fluorescence Assay Interference-The Case for Diaphorase. Assay Drug Dev Technol. 2016 Apr;14(3):175-9. doi: 10.1089/adt.2016.707. Review. PubMed PMID: 27078679; PubMed Central PMCID: PMC4840916. 4: Coban AY, Deveci A, Sunter AT, Palomino JC, Martin A. Resazurin microtiter assay for isoniazid, rifampicin, ethambutol and streptomycin resistance detection in Mycobacterium tuberculosis: Updated meta-analysis. Int J Mycobacteriol. 2014 Dec;3(4):230-41. doi: 10.1016/j.ijmyco.2014.09.002. Epub 2014 Sep 29. Review. PubMed PMID: 26786621. 5: Alnimr AM. Dormancy models for Mycobacterium tuberculosis: A minireview. Braz J Microbiol. 2015 Jul 1;46(3):641-7. doi: 10.1590/S1517-838246320140507. eCollection 2015 Jul-Sep. Review. PubMed PMID: 26413043; PubMed Central PMCID: PMC4568887. 6: Pantanella F, Valenti P, Natalizi T, Passeri D, Berlutti F. Analytical techniques to study microbial biofilm on abiotic surfaces: pros and cons of the main techniques currently in use. Ann Ig. 2013 Jan-Feb;25(1):31-42. doi: 10.7416/ai.2013.1904. Review. PubMed PMID: 23435778. 7: Rampersad SN. Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 2012;12(9):12347-60. doi: 10.3390/s120912347. Epub 2012 Sep 10. Review. PubMed PMID: 23112716; PubMed Central PMCID: PMC3478843. 8: Vega-Avila E, Pugsley MK. An overview of colorimetric assay methods used to assess survival or proliferation of mammalian cells. Proc West Pharmacol Soc. 2011;54:10-4. Review. PubMed PMID: 22423572. 9: Matteucci E, Giampietro O. Flow cytometry study of leukocyte function: analytical comparison of methods and their applicability to clinical research. Curr Med Chem. 2008;15(6):596-603. Review. PubMed PMID: 18336274. 10: Comhaire F, Vermeulen L. Human semen analysis. Hum Reprod Update. 1995 Jul;1(4):343-62. Review. PubMed PMID: 9080212.

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