DiFMUAc| CAS# | fluorescent substrate

MedKoo Cat#: 130005 | Name: DiFMUAc

Description:

WARNING: This product is for research use only, not for human or veterinary use.

DiFMUAc is a fluorescent substrate of the oncoprotein and Parkinson's disease-associated enzyme DJ-1/PARK7. As opposed to conventional methods of in-direct colorimetric readouts, the deacetylation of DiFMUAc has been used to develop a PARK7 activity assay, which improved results and allowed for continuous monitoring of PARK7 activity.

Chemical Structure

DiFMUAc

CAS#N/A

Theoretical Analysis

MedKoo Cat#: 130005

Name: DiFMUAc

CAS#: N/A

Chemical Formula: C12H8F2O4

Exact Mass: 254.0391

Molecular Weight: 254.19

Elemental Analysis: C, 56.70; H, 3.17; F, 14.95; O, 25.18

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

Bulk Inquiry

Related CAS #

No Data

Synonym

DiFMUAc; DiF-MUAc

IUPAC/Chemical Name

6,8-Difluoro-4-methyl-2-oxo-2H-chromen-7-yl acetate

InChi Key

CVENZCRRFMDFOK-UHFFFAOYSA-N

InChi Code

InChI=1S/C12H9FO4/c1-6-3-12(15)17-10-5-11(16-7(2)14)9(13)4-8(6)10/h3-5H,1-2H3

SMILES Code

CC(OC1=C(F)C(O2)=C(C=C1F)C(C)=CC2=O)=O

Appearance

To be determined

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Through the deacetylation of DiFMUAc a PARK7 activity assay has been developed. PARK7/DJ1 is a great target for therapeutics as it has been linked to early-onset Parkinson's disease, chemoresistance, as well as leading to an upregulation of various cancers.

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 254.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Jia Y, Kim RQ, Kooij R, Ovaa H, Sapmaz A, Geurink PP. Chemical Toolkit for PARK7: Potent, Selective, and High-Throughput. J Med Chem. 2022 Oct 13;65(19):13288-13304. doi: 10.1021/acs.jmedchem.2c01113. Epub 2022 Sep 23. PMID: 36149939; PMCID: PMC9574860. Maksimovic I, Finkin-Groner E, Fukase Y, Zheng Q, Sun S, Michino M, Huggins DJ, Myers RW, David Y. Deglycase-activity oriented screening to identify DJ-1 inhibitors. RSC Med Chem. 2021 Jun 2;12(7):1232-1238. doi: 10.1039/d1md00062d. PMID: 34355187; PMCID: PMC8292988.