CAS#214491-43-7

MedKoo Cat#: 527018 | Name: DiFMUP

Description:

WARNING: This product is for research use only, not for human or veterinary use.

DiFMUP is a fluorogenic substrate for protein tyrosine phosphatases.

Chemical Structure

DiFMUP

CAS#214491-43-7

Theoretical Analysis

MedKoo Cat#: 527018

Name: DiFMUP

CAS#: 214491-43-7

Chemical Formula: C10H7F2O6P

Exact Mass: 291.9948

Molecular Weight: 292.13

Elemental Analysis: C, 41.12; H, 2.42; F, 13.01; O, 32.86; P, 10.60

Price and Availability

This product is currently not in stock but may be available through custom synthesis. To ensure cost efficiency, the minimum order quantity is 1 gram. The estimated lead time is 2 to 4 months, with pricing dependent on the complexity of the synthesis (typically high for intricate chemistries). Quotes for quantities below 1 gram will not be provided. To request a quote, please click the button below. Note: If this product becomes available in stock in the future, pricing will be listed accordingly.

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Related CAS #

No Data

Synonym

DiFMUP

IUPAC/Chemical Name

(6,8-Difluoro-4-methyl-2-oxochromen-7-yl) dihydrogen phosphate

InChi Key

DZANYXOTJVLAEE-UHFFFAOYSA-N

InChi Code

InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16)

SMILES Code

O=P(O)(OC1=C(F)C(O2)=C(C=C1F)C(C)=CC2=O)O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Instruction

View handling instruction

Preparing Stock Solutions

The following data is based on the product molecular weight 292.13 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

1: Zhang H, Xiao P, Wong YT, Shen W, Chhabra M, Peltier R, Jiang Y, He Y, He J, Tan Y, Xie Y, Ho D, Lam YW, Sun J, Sun H. Construction of an alkaline phosphatase-specific two-photon probe and its imaging application in living cells and tissues. Biomaterials. 2017 Sep;140:220-229. doi: 10.1016/j.biomaterials.2017.06.032. Epub 2017 Jun 23. PubMed PMID: 28662402. 2: Queralt E, Rodriguez-Rodriguez JA. A New Methodology for the Quantification of In Vivo Cdc14 Phosphatase Activity. Methods Mol Biol. 2017;1505:89-96. PubMed PMID: 27826859. 3: Mentel M, Badea RA, Necula-Petrareanu G, Mallikarjuna ST, Ionescu AE, Szedlacsek SE. Expression, Purification, and Kinetic Analysis of PTP Domains. Methods Mol Biol. 2016;1447:39-66. doi: 10.1007/978-1-4939-3746-2_3. PubMed PMID: 27514799. 4: Jully V, Mathot F, Moniotte N, Préat V, Lemoine D. Mechanisms of Antigen Adsorption Onto an Aluminum-Hydroxide Adjuvant Evaluated by High-Throughput Screening. J Pharm Sci. 2016 Jun;105(6):1829-1836. doi: 10.1016/j.xphs.2016.03.032. PubMed PMID: 27238481. 5: Sancenon V, Goh WH, Sundaram A, Er KS, Johal N, Mukhina S, Carr G, Dhakshinamoorthy S. Development, validation and quantitative assessment of an enzymatic assay suitable for small molecule screening and profiling: A case-study. Biomol Detect Quantif. 2015 Apr 27;4:1-9. doi: 10.1016/j.bdq.2015.03.001. eCollection 2015 Jun. PubMed PMID: 27077032; PubMed Central PMCID: PMC4822204. 6: Irani S, Yogesha SD, Mayfield J, Zhang M, Zhang Y, Matthews WL, Nie G, Prescott NA, Zhang YJ. Structure of Saccharomyces cerevisiae Rtr1 reveals an active site for an atypical phosphatase. Sci Signal. 2016 Mar 1;9(417):ra24. doi: 10.1126/scisignal.aad4805. PubMed PMID: 26933063; PubMed Central PMCID: PMC4801511. 7: Greiner R, Dick TP. Real-time assays for monitoring the influence of sulfide and sulfane sulfur species on protein thiol redox states. Methods Enzymol. 2015;555:57-77. doi: 10.1016/bs.mie.2014.11.020. Epub 2015 Jan 10. PubMed PMID: 25747475. 8: Lerner N, Beit-Yannai E. Cross-talk between ciliary epithelium and trabecular meshwork cells in-vitro: a new insight into glaucoma. PLoS One. 2014 Nov 12;9(11):e112259. doi: 10.1371/journal.pone.0112259. eCollection 2014. PubMed PMID: 25389776; PubMed Central PMCID: PMC4229184. 9: Mußbach F, Henklein P, Westermann M, Settmacher U, Böhmer FD, Kaufmann R. Proteinase-activated receptor 1- and 4-promoted migration of Hep3B hepatocellular carcinoma cells depends on ROS formation and RTK transactivation. J Cancer Res Clin Oncol. 2015 May;141(5):813-25. doi: 10.1007/s00432-014-1863-4. Epub 2014 Nov 6. PubMed PMID: 25373316. 10: Moretti DM, Ahuja LG, Nunes RD, Cudischevitch CO, Daumas-Filho CR, Medeiros-Castro P, Ventura-Martins G, Jablonka W, Gazos-Lopes F, Senna R, Sorgine MH, Hartfelder K, Capurro M, Atella GC, Mesquita RD, Silva-Neto MA. Molecular analysis of Aedes aegypti classical protein tyrosine phosphatases uncovers an ortholog of mammalian PTP-1B implicated in the control of egg production in mosquitoes. PLoS One. 2014 Aug 19;9(8):e104878. doi: 10.1371/journal.pone.0104878. eCollection 2014. PubMed PMID: 25137153; PubMed Central PMCID: PMC4138107. 11: Lopez SM, Hodgson MC, Packianathan C, Bingol-Ozakpinar O, Uras F, Rosen BP, Agoulnik IU. Determinants of the tumor suppressor INPP4B protein and lipid phosphatase activities. Biochem Biophys Res Commun. 2013 Oct 18;440(2):277-82. doi: 10.1016/j.bbrc.2013.09.077. Epub 2013 Sep 23. PubMed PMID: 24070612; PubMed Central PMCID: PMC4578723. 12: Wang J, Morabito K, Erkers T, Tripathi A. Capture and separation of biomolecules using magnetic beads in a simple microfluidic channel without an external flow device. Analyst. 2013 Nov 7;138(21):6573-81. doi: 10.1039/c3an01166f. PubMed PMID: 24051541. 13: Kulkarni RA, Vellore NA, Bliss MR, Stanford SM, Falk MD, Bottini N, Baron R, Barrios AM. Substrate selection influences molecular recognition in a screen for lymphoid tyrosine phosphatase inhibitors. Chembiochem. 2013 Sep 2;14(13):1640-7. doi: 10.1002/cbic.201300273. Epub 2013 Aug 16. PubMed PMID: 23956195; PubMed Central PMCID: PMC3874405. 14: Sotoud H, Gribbon P, Ellinger B, Reinshagen J, Boknik P, Kattner L, El-Armouche A, Eschenhagen T. Development of a colorimetric and a fluorescence phosphatase-inhibitor assay suitable for drug discovery approaches. J Biomol Screen. 2013 Sep;18(8):899-909. doi: 10.1177/1087057113486000. Epub 2013 Apr 19. PubMed PMID: 23606651. 15: Bayley R, Yang P, Buckley CD, Young SP. Measuring the specific activity of the protein tyrosine phosphatase Lyp. J Immunol Methods. 2013 Feb 28;388(1-2):33-9. doi: 10.1016/j.jim.2012.11.011. Epub 2012 Dec 3. PubMed PMID: 23219421. 16: Kameshita I. In-gel phosphatase assay using fluorogenic and radioactive substrates. Curr Protoc Protein Sci. 2011 Aug;Chapter 13:Unit13.12. doi: 10.1002/0471140864.ps1312s65. PubMed PMID: 21842466. 17: Gade TP, Motley MW, Beattie BJ, Bhakta R, Boskey AL, Koutcher JA, Mayer-Kuckuk P. Imaging of alkaline phosphatase activity in bone tissue. PLoS One. 2011;6(7):e22608. doi: 10.1371/journal.pone.0022608. Epub 2011 Jul 25. PubMed PMID: 21799916; PubMed Central PMCID: PMC3143164. 18: He TP, Liang NC, Lin XC, Liu W. [Effect of seven kinds of flavonoids on recombinant human protein tyrosine phosphatase]. Zhong Yao Cai. 2010 May;33(5):771-4. Chinese. PubMed PMID: 20873564. 19: Eum JH, Bottjen RC, Pruijssers AJ, Clark KD, Strand MR. Characterization and kinetic analysis of protein tyrosine phosphatase-H2 from Microplitis demolitor bracovirus. Insect Biochem Mol Biol. 2010 Sep;40(9):690-8. doi: 10.1016/j.ibmb.2010.07.003. Epub 2010 Aug 5. PubMed PMID: 20691785. 20: Kameshita I, Baba H, Umeda Y, Sueyoshi N. In-gel protein phosphatase assay using fluorogenic substrates. Anal Biochem. 2010 May 1;400(1):118-22. doi: 10.1016/j.ab.2009.12.035. Epub 2010 Jan 4. PubMed PMID: 20045670.