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MedKoo Cat#: 526654 | Name: ML-098
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

ML-098, also known as CID-7345532, is an activator of the GTP-binding protein Rab7 (EC50 = 77.6 nM). ML098 (CID-7345532) functions by increasing the affinity of the GTPases for guanine nucleotides, leading to the hypothesis that these activators interact with GTPases bind to an allosteric binding site localized between switch regions I and II. .

Chemical Structure

ML-098
ML-098
CAS#878978-76-8

Theoretical Analysis

MedKoo Cat#: 526654

Name: ML-098

CAS#: 878978-76-8

Chemical Formula: C19H19NO3

Exact Mass: 309.1365

Molecular Weight: 309.37

Elemental Analysis: C, 73.77; H, 6.19; N, 4.53; O, 15.51

Price and Availability

Size Price Availability Quantity
25mg USD 150.00 Ready to ship
50mg USD 250.00 Ready to ship
100mg USD 450.00 Ready to ship
200mg USD 650.00 Ready to ship
500mg USD 850.00 Ready to ship
1g USD 1,250.00 Ready to ship
2g USD 2,150.00 Ready to ship
5g USD 3,850.00 2 weeks
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Related CAS #
No Data
Synonym
ML-098; ML 098; ML098; CID-7345532; CID 7345532; CID7345532.
IUPAC/Chemical Name
1-(2-(2,5-dimethylphenoxy)ethyl)-1H-indole-3-carboxylic acid
InChi Key
LILIWWFBJKBUCO-UHFFFAOYSA-N
InChi Code
InChI=1S/C19H19NO3/c1-13-7-8-14(2)18(11-13)23-10-9-20-12-16(19(21)22)15-5-3-4-6-17(15)20/h3-8,11-12H,9-10H2,1-2H3,(H,21,22)
SMILES Code
O=C(C1=CN(CCOC2=CC(C)=CC=C2C)C3=C1C=CC=C3)O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Biological target:
ML-098 (CID-7345532) is an activator of the GTP-binding protein Rab7 with an EC50 of 77.6 nM. The papillary thyroid carcinoma cell line TPC-1 was cultured, and the cells were divided into control group, the 1,25-(OH)2D3 group, and the 1,25-(OH)2D3 + ML098 (Ras agonist) group.
In vitro activity:
The objective of this study was to explore the effects of 1,25-dihydroxy vitamin D3 [1,25-(OH)2D3] on the proliferation and apoptosis of papillary thyroid carcinoma and to investigate its possible mechanism. Compared with the drug group, the cloning and migration biological activity of cells in the 1,25-(OH)2D3 + ML-098 group was significantly enhanced (p < 0.05). The assay results showed that 1,25-(OH)2D3 could reduce the activity of the Ras-MEK-ERK pathway. To further verify this effect, we added Ras agonist (ML-098) treatment and observed the above indicators again. The ability of cell proliferation, colony formation, and migration in 1,25-(OH)2D3 + ML-098 group was increased in contrast to the 1,25-(OH)2D3 group, and the number of apoptotic cells was decreased. The expression of Ki67 protein was increased, while the expression of Caspase-3 protein was decreased (p < 0.05). The anticancer effect of the drug was reversed by ML-098. Moreover, the expression level of Ras was upregulated in 1,25-(OH)2D3 + ML-098 group than in the 1,25-(OH)2D3 group, the phosphorylation level of p-ERK1/2 and p-MEK was increased and the effect of 1,25-(OH)2D3 on the Ras-MEK-ERK pathway was reversed by ML-098 (p < 0.05). Steroids. 2020 Jul;159:108585. https://pubmed.ncbi.nlm.nih.gov/31982425/
In vivo activity:
TBD
Solvent mg/mL mM comments
Solubility
DMSO 53.0 171.45
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 309.37 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Li B, Lv L, Li W. 1,25-Dihydroxy vitamin D3 inhibits the Ras-MEK-ERK pathway and regulates proliferation and apoptosis of papillary thyroid carcinoma. Steroids. 2020 Jul;159:108585. doi: 10.1016/j.steroids.2020.108585. Epub 2020 Jan 23. PMID: 31982425. 2. Surviladze Z, Ursu O, Miscioscia F, Curpan R, Halip L, Bologa C, Oprea T, Waller A, Strouse J, Salas V, Wu Y, Edwards B, Wandinger-Ness A, Sklar L. Three small molecule pan activator families of Ras-related GTPases. 2009 May 18 [updated 2010 Sep 2]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010–. PMID: 21433375.
In vitro protocol:
1. Li B, Lv L, Li W. 1,25-Dihydroxy vitamin D3 inhibits the Ras-MEK-ERK pathway and regulates proliferation and apoptosis of papillary thyroid carcinoma. Steroids. 2020 Jul;159:108585. doi: 10.1016/j.steroids.2020.108585. Epub 2020 Jan 23. PMID: 31982425. 2. Surviladze Z, Ursu O, Miscioscia F, Curpan R, Halip L, Bologa C, Oprea T, Waller A, Strouse J, Salas V, Wu Y, Edwards B, Wandinger-Ness A, Sklar L. Three small molecule pan activator families of Ras-related GTPases. 2009 May 18 [updated 2010 Sep 2]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010–. PMID: 21433375.
In vivo protocol:
TBD
1: Surviladze Z, Ursu O, Miscioscia F, Curpan R, Halip L, Bologa C, Oprea T, Waller A, Strouse J, Salas V, Wu Y, Edwards B, Wandinger-Ness A, Sklar L. Three small molecule pan activator families of Ras-related GTPases. 2009 May 18 [updated 2010 Sep 2]. Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-. Available from http://www.ncbi.nlm.nih.gov/books/NBK47359/ PubMed PMID: 21433375.