SPOP-i-6lc is a selective SPOP E3 ubiquitin ligase inhibitor (IC50 = 2.1 μM and 3.5 μM, in A498 and OS-RC-2 cell lines, respectively). In vitro, SPOP-i-6lc suppresses viability and proliferation of A498 and OS-RC-2 kidney cancer cell lines. SPOP-i-6lc leads to the accumulation of tumor suppressors PTEN and DUSP7 and decreased levels of phosphorylated AKT and ERK in clear-cell renal cell carcinoma (ccRCC) cell lines.
MedKoo Cat#: 466242
Name: SPOP-i-6lc
CAS#: 2136270-56-7
Chemical Formula: C26H31N7O2S
Exact Mass: 505.2260
Molecular Weight: 505.64
Elemental Analysis: C, 61.76; H, 6.18; N, 19.39; O, 6.33; S, 6.34
| Solvent | mg/mL | mM | comments | |
|---|---|---|---|---|
| Solubility | ||||
| Ethanol | 2.5 | 5.00 | ||
| DMSO | 5.1 | 10.00 |
The following data is based on the product molecular weight 505.64 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
