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MedKoo Cat#: 510272 | Name: Y27632 HCl
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Y27632 is a selective ROCK inhibitor, which inhibits ET-1-induced increases in natriuretic peptide production, cell size, protein synthesis, and myofibrillar organization. Y27632 prevents dimethylnitrosamine-induced hepatic fibrosis in rats, increases apoptosis and disrupts the actin cortical mat in embryonic avian corneal epithelium, affects initial heart myofibrillogenesis in cultured chick blastoderm, promotes the proliferation and cell cycle progression of cultured astrocyte from spinal cord.

Chemical Structure

Y27632 HCl
Y27632 HCl
CAS#129830-38-2 (2HCl)

Theoretical Analysis

MedKoo Cat#: 510272

Name: Y27632 HCl

CAS#: 129830-38-2 (2HCl)

Chemical Formula: C14H23Cl2N3O

Exact Mass: 247.1685

Molecular Weight: 320.26

Elemental Analysis: C, 52.51; H, 7.24; Cl, 22.14; N, 13.12; O, 5.00

Price and Availability

Size Price Availability Quantity
10mg USD 110.00 Ready to ship
25mg USD 200.00 Ready to ship
50mg USD 350.00 Ready to ship
100mg USD 600.00 Ready to ship
200mg USD 1,050.00 Ready to ship
500mg USD 1,850.00 Ready to ship
1g USD 2,850.00 Ready to ship
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Related CAS #
331752-47-7 (HCl hydrate) 129830-38-2 (2HCl) 146986-50-7 (free base) 138381-45-0 (racemate HCl) 310898-86-3 (recamte free base)
Synonym
Y27632; Y-27632; Y 27632; Y27632 HCl; Y27632 dihydrochloride
IUPAC/Chemical Name
(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide dihydrochloride
InChi Key
IDDDVXIUIXWAGJ-LJDSMOQUSA-N
InChi Code
InChI=1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11-,12-;;/m1../s1
SMILES Code
O=C([C@H]1CC[C@H]([C@H](N)C)CC1)NC2=CC=NC=C2.[H]Cl.[H]Cl
Appearance
white to off-white solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
 Related CAS#  CAS#129830-38-2 ( Y27632 2HCl); CAS3146986-50-7 ( Y27632 free base)       
Biological target:
Y-27632 dihydrochloride is an ATP-competitive inhibitor of ROCK-I and ROCK-II, with Kis of 220 and 300 nM, respectively.
In vitro activity:
First this study treated HDFs with or without Y-27632 in the presence of 100 ng/ml IGFBP-5, a concentration close to what was found in the conditioned media after Y-27632 treatment. After 48 h, this study analyzed the senescence of HDFs by SA-β-gal staining and found that treatment with Y-27632 and/or IGFBP-5 significantly induced the senescence of HDFs (Figure 7A, 7B). In contrast, when HDFs were treated with Y-27632 with or without the knockdown of IGFBP-5 (siIGFBP-5), which was confirmed by RT-PCR analysis (Supplementary Figure 7A), the knockdown of IGFBP-5 significantly blocked the increased cellular senescence induced by Y-27632 (Figure 7C, 7D). These results suggest that the prolonged treatment of HDFs with Y-27632 increases cellular senescence probably by enhancing the expression and production of IGFBP-5. Reference: Aging (Albany NY). 2020 Aug 31; 12(16): 16621–16646. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485707/
In vivo activity:
In conclusion, this study demonstrated the role of the ROCK signaling pathway in the survival of mouse SGSCs. By inhibiting the ROCK signaling pathway through the chemical inhibitor Y-27632, dissociation-induced cell death of SGSCs was significantly reduced. In addition, ECM-based experiments revealed that the disruption of the cell–ECM interactions is associated with increased cell death of SGSCs. Reference: Molecules. 2021 May; 26(9): 2658. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8124333/
Solvent mg/mL mM
Solubility
DMSO 39.5 123.37
DMF 20.0 62.45
Ethanol 8.5 26.54
PBS (pH 7.2) 10.0 31.22
Water 73.3 229.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 320.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Li X, Zhou Q, Wang S, Wang P, Li J, Xie Z, Liu C, Wen J, Wu X. Prolonged treatment with Y-27632 promotes the senescence of primary human dermal fibroblasts by increasing the expression of IGFBP-5 and transforming them into a CAF-like phenotype. Aging (Albany NY). 2020 Aug 25;12(16):16621-16646. doi: 10.18632/aging.103910. Epub 2020 Aug 25. PMID: 32843583; PMCID: PMC7485707. 2. Kim K, Min S, Kim D, Kim H, Roh S. A Rho Kinase (ROCK) Inhibitor, Y-27632, Inhibits the Dissociation-Induced Cell Death of Salivary Gland Stem Cells. Molecules. 2021 May 1;26(9):2658. doi: 10.3390/molecules26092658. PMID: 34062818; PMCID: PMC8124333.
In vitro protocol:
1. Li X, Zhou Q, Wang S, Wang P, Li J, Xie Z, Liu C, Wen J, Wu X. Prolonged treatment with Y-27632 promotes the senescence of primary human dermal fibroblasts by increasing the expression of IGFBP-5 and transforming them into a CAF-like phenotype. Aging (Albany NY). 2020 Aug 25;12(16):16621-16646. doi: 10.18632/aging.103910. Epub 2020 Aug 25. PMID: 32843583; PMCID: PMC7485707.
In vivo protocol:
1. Kim K, Min S, Kim D, Kim H, Roh S. A Rho Kinase (ROCK) Inhibitor, Y-27632, Inhibits the Dissociation-Induced Cell Death of Salivary Gland Stem Cells. Molecules. 2021 May 1;26(9):2658. doi: 10.3390/molecules26092658. PMID: 34062818; PMCID: PMC8124333.
1: Lordier L, Di Buduo CA, Kauskot A, Balayn N, Lavenu-Bombled C, Baschieri F, Proulle V, Oyarzun CPM, Careddu F, Biunno I, Manoliu T, Rameau P, Plo I, Papadopoulos N, Constantinescu S, Vainchenker W, Nguyen GN, Ballerini P, Favier R, Balduini A, Raslova H. Increased RhoA pathway activation downstream of αIIbβ3/SRC contributes to heterozygous Bernard Soulier syndrome. Haematologica. 2025 Mar 6. doi: 10.3324/haematol.2024.286424. Epub ahead of print. PMID: 40045897. 2: Holland EJ, Yeu E, Giegengack M, Berdahl J, Jordan E, Quesada R, Quesada G, Kinoshita S, Sotozono C, Toda M, Ueno M, Lacoste A, Qu X, Berenshteyn F, Blender N, Goldstein MH. Escalón: A Prospective Randomized Trial of Corneal Endothelial Cell Therapy in Subjects With Corneal Edema. Cornea. 2025 Mar 3. doi: 10.1097/ICO.0000000000003844. Epub ahead of print. PMID: 40032264. 3: Olukman M, Can C, Ülker S, Uyanikgil Y, Çavuşoğlu T, Düzenli N, Coşkunsever D, Kozcu FG. The effects of the urotensin-II receptor antagonist palosuran treatment on the corpora cavernosa of streptozotocin-induced diabetic rats. Asian J Urol. 2025 Jan;12(1):116-126. doi: 10.1016/j.ajur.2024.02.010. Epub 2024 May 17. PMID: 39990066; PMCID: PMC11840324. 4: Wang D, Wu J, Xu Z, Jia J, Lai Y, He Z. Increased Matrix Stiffness Promotes Slow Muscle Fibre Regeneration After Skeletal Muscle Injury. J Cell Mol Med. 2025 Feb;29(4):e70423. doi: 10.1111/jcmm.70423. PMID: 39969079; PMCID: PMC11837045. 5: Khavandegar A, Ahmadi NS, Mousavi MA, Ramezani Z, Khodadoust E, Hasan Zadeh Tabatabaei MS, Hasanpour Segherlou Z, Zeinaddini-Meymand A, Nasehi F, Moafi M, RayatSanati K, Masoomi R, Hamidi S, Pourkhodadad S, Rahimi-Movaghar V. The potential role of RhoA/ROCK-inhibition on locomotor recovery after spinal cord injury: a systematic review of in-vivo studies. Spinal Cord. 2025 Feb 16. doi: 10.1038/s41393-025-01064-2. Epub ahead of print. PMID: 39956860. 6: Wen J, Sun Y, Ma L, Zu T, Wang N, Zhang T, Liang J, Zhang Y, Lu H, Wu Y, Zhang S. Y-27632 Suppresses the Growth and Migration of Oral Squamous Cell Carcinoma, but Upregulates Autophagy by Suppressing mTOR Effectors. J Oral Pathol Med. 2025 Feb 8. doi: 10.1111/jop.13603. Epub ahead of print. PMID: 39921531. 7: Lage L, Rodriguez-Perez AI, Labandeira-Garcia JL, Dominguez-Meijide A. Fasudil inhibits α-synuclein aggregation through ROCK-inhibition-mediated mechanisms. Neurotherapeutics. 2025 Mar;22(2):e00544. doi: 10.1016/j.neurot.2025.e00544. Epub 2025 Feb 5. PMID: 39915220. 8: Shvetsova AA, Shateeva VS, Khlystova MA, Makukha YA, Tarasova OS, Gaynullina DK. NADPH oxidase derived ROS promote arterial contraction in early postnatal rats by activation of L-type voltage-gated Ca2+ channels. Free Radic Res. 2025 Jan;59(1):49-60. doi: 10.1080/10715762.2024.2448483. Epub 2024 Dec 30. PMID: 39879138. 9: Chen JQ, Zheng M, Li W, Zhou J, Man XY. Regulation of involucrin and signalling pathway in scleroderma epidermal keratinocytes. Postepy Dermatol Alergol. 2024 Dec;41(6):604-609. doi: 10.5114/ada.2024.144841. Epub 2024 Nov 8. PMID: 39877111; PMCID: PMC11770575. 10: Ho WT, Chang JS, Lei CJ, Chen TC, Wang JK, Chang SW, Yang MH, Jou TS, Wang IJ. ROCK Inhibitor Enhances Resilience Against Metabolic Stress Through Increasing Bioenergetic Capacity in Corneal Endothelial Cells. Invest Ophthalmol Vis Sci. 2025 Jan 2;66(1):51. doi: 10.1167/iovs.66.1.51. PMID: 39847368; PMCID: PMC11759620. 11: Zou Y, Zeng X, Wang K, Ye J, Zhao Y, Jin H, Zhang J, Cheng G, Nie X. CD271 regulates osteogenic differentiation of ectomesenchymal stem cells via the RhoA/ROCK signaling pathway. Int Immunopharmacol. 2025 Feb 20;148:114068. doi: 10.1016/j.intimp.2025.114068. Epub 2025 Jan 17. PMID: 39826451. 12: Sonoi R, Kamihira M. Formation of epithelial polarity on the fluorinated-oil microdroplet surface by regulating cell adhesion. J Biosci Bioeng. 2025 Mar;139(3):234-241. doi: 10.1016/j.jbiosc.2024.12.009. Epub 2025 Jan 11. PMID: 39800607. 13: Wang P, Shen S, Guo Y, Cao J, Zhu D, Xie M, Yu Q, Cui Z, Liu S, Zhang J, Chen J. Rho kinase inhibitor Y-27632 and dual media culture approach promote the construction and transplantation of rabbit limbal epithelial cell sheets via cell spheroid culture and auto-bioprinting. Acta Biomater. 2025 Mar 1;194:140-152. doi: 10.1016/j.actbio.2025.01.022. Epub 2025 Jan 10. PMID: 39800095. 14: Wang J, Tao Q, Huang K, Wang Y, Hu L, Ren A, Wang H, Wan Y, Li J, Yi L, Ruan Y, Wanyan Z, Wu F, Zhai Z, Liu C. Chemotherapy-induced cellular senescence promotes stemness of aggressive B-cell non-Hodgkin's lymphoma via CCR7/ARHGAP18/IKBα signaling activation. J Immunother Cancer. 2025 Jan 7;13(1):e009356. doi: 10.1136/jitc-2024-009356. PMID: 39773566; PMCID: PMC11749403. 15: Bouzid T, Kim E, Riehl BD, Yang R, Saraswathi V, Kim JK, Lim JY. Mechanical Stretch Control of Adipocyte AKT Signaling and the Role of FAK and ROCK Mechanosensors. Bioengineering (Basel). 2024 Dec 16;11(12):1279. doi: 10.3390/bioengineering11121279. PMID: 39768098; PMCID: PMC11673816. 16: Umetsu A, Ida Y, Sato T, Higashide M, Nishikiori N, Furuhashi M, Ohguro H, Watanabe M. RHO-Associated Coiled-Coil-Containing Protein Kinase Inhibitors Significantly Modulate the Epithelial-Mesenchymal Transition Induced by TGF-β2 in the 2-D and 3-D Cultures of Human Corneal Stroma Fibroblasts. Biomedicines. 2024 Dec 6;12(12):2784. doi: 10.3390/biomedicines12122784. PMID: 39767691; PMCID: PMC11673340. 17: Dai J, Rayana NP, Peng M, Sugali CK, Harvey DH, Dhamodaran K, Yu E, Dalloul JM, Liu S, Mao W. Inhibition of pterygium cell fibrosis by the Rho kinase inhibitor. Sci Rep. 2024 Dec 28;14(1):30930. doi: 10.1038/s41598-024-81659-6. PMID: 39730553; PMCID: PMC11680712. 18: Liu L, Chen T, Xie Z, Zhang Y, He C, Huang Y. Butyric acid alleviates LPS- induced intestinal mucosal barrier damage by inhibiting the RhoA/ROCK2/MLCK signaling pathway in Caco2 cells. PLoS One. 2024 Dec 26;19(12):e0316362. doi: 10.1371/journal.pone.0316362. PMID: 39724098; PMCID: PMC11670954. 19: Mekchay P, Ingrungruanglert P, Leela-Adisorn N, Uaprasert N, Israsena N, Rojnuckarin P. Roles of ROCK/Myosin Pathway in Macrothrombocytopenia in Bernard- Soulier Syndrome. Thromb Haemost. 2024 Dec 18. doi: 10.1055/a-2474-5644. Epub ahead of print. PMID: 39694061. 20: Zelko IN, Hussain A, Malovichko MV, Wickramasinghe N, Srivastava S. Benzene metabolites increase vascular permeability by activating heat shock proteins and Rho GTPases. bioRxiv [Preprint]. 2024 Dec 7:2024.12.04.626801. doi: 10.1101/2024.12.04.626801. PMID: 39677674; PMCID: PMC11643022.

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