Rf470DL is a blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria. Rf470DL fluoresces only when it becomes incorporated into the peptidoglycan, enabling no wash experiments. Excitation/emission λ ~470/620 nm; quantum yield = 0.042; extinction coefficient 33,106 M-1 cm-1 (measured in PBS containing 50% glycerol).
MedKoo Cat#: 465601
Name: Rf470DL
CAS#: 2758364-03-1 (HCl)
Chemical Formula: C26H31ClN4O5S
Exact Mass: 546.1704
Molecular Weight: 547.07
Elemental Analysis: C, 57.08; H, 5.71; Cl, 6.48; N, 10.24; O, 14.62; S, 5.86
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 54.7 | 100.00 |
The following data is based on the product molecular weight 547.07 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
