seMpai is a novel AkaLumine analog, acting as an NIR emission luciferin with the enhanced solubility in water. seMpai exhibits an emission maximum at 675 nm upon the firefly luciferin-luciferase (L-L) reaction. seMpai bioluminescence imaging (BLI) could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.
MedKoo Cat#: 465342
Name: seMpai
CAS#: 1821155-77-4 (free acid)
Chemical Formula: C15H17N3O2S
Exact Mass: 303.1041
Molecular Weight: 303.38
Elemental Analysis: C, 59.39; H, 5.65; N, 13.85; O, 10.55; S, 10.57
The following data is based on the product molecular weight 303.38 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
