Pefloxacin Free Base | CAS# 70458-92-3 (free base) | fluoroquinolone antibacterial agent

MedKoo Cat#: 414149 | Name: Pefloxacin Free Base

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Pefloxacin Free Base is a synthetic broad-spectrum fluoroquinolone antibacterial agent active against most gram-negative and gram-positive bacteria.

Chemical Structure

Pefloxacin Free Base

CAS#70458-92-3 (free base)

Theoretical Analysis

MedKoo Cat#: 414149

Name: Pefloxacin Free Base

CAS#: 70458-92-3 (free base)

Chemical Formula: C17H20FN3O3

Exact Mass: 333.1489

Molecular Weight: 333.36

Elemental Analysis: C, 61.25; H, 6.05; F, 5.70; N, 12.61; O, 14.40

Price and Availability

Size	Price	Availability
1mg	USD 220.00	2 Weeks
2mg	USD 360.00	2 Weeks
5mg	USD 590.00	2 Weeks
10mg	USD 870.00	2 Weeks
25mg	USD 1,390.00	2 Weeks

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Related CAS #

70458-95-6 (mesylate) 70458-92-3 (free base)

Synonym

Pefloxacin Free Base; 1584 RB; 1584-RB; 1584RB; EU5306; EU-5306; EU 5306

IUPAC/Chemical Name

3-Quinolinecarboxylic acid, 1,4-dihydro-1-ethyl-6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-

InChi Key

FHFYDNQZQSQIAI-UHFFFAOYSA-N

InChi Code

InChI=1S/C17H20FN3O3/c1-3-20-10-12(17(23)24)16(22)11-8-13(18)15(9-14(11)20)21-6-4-19(2)5-7-21/h8-10H,3-7H2,1-2H3,(H,23,24)

SMILES Code

O=C(C1=CN(CC)C2=C(C=C(F)C(N3CCN(C)CC3)=C2)C1=O)O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Pefloxacin is a an antibacterial agent and prevents bacterial DNA replication by inhibiting DNA gyrase (topoisomerse).

In vitro activity:

PFLX (pefloxacin) was photodegraded in 5h and forms photoproduct under UVA exposure. At the non photocytotoxic dose PFLX, shows reduced phagocytosis activity, NO (nitric oxide) production, large vacuole formation and down regulated IL-6, TNF-α and IL-1 in BALB/c macrophages at both genes and proteins levels. At higher doses (photocytotoxic doses), PFLX induced a concentration dependent decrease in cell viability of human keratinocyte cell line (HaCaT) and peritoneal macrophages of BALB/c mice. Reference: J Photochem Photobiol B. 2018 Jan;178:593-605. https://pubmed.ncbi.nlm.nih.gov/29275239/

In vivo activity:

The aim of this study was to investigate the changes in the number of serotonergic immunoreactive fibers and mast cells after pefloxacin treatment in the parotid and sublingual glands of rats to detect the possible neurotoxic effect of pefloxacin. These results suggest that pefloxacin treatment can modify the finely controlled communication between the immune- and the peripheral nervous systems, resulting neurogenic inflammatory process. Reference: Drug Chem Toxicol. 2020 Sep;43(5):496-503. https://pubmed.ncbi.nlm.nih.gov/30257570/

Preparing Stock Solutions

The following data is based on the product molecular weight 333.36 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Singh J, Srivastva AK, Mandal P, Chandra S, Dubey D, Dwivedi A, Chopra D, Tripathi A, Ray RS. Under ambient UVA exposure, pefloxacin exhibits both immunomodulatory and genotoxic effects via multiple mechanisms. J Photochem Photobiol B. 2018 Jan;178:593-605. doi: 10.1016/j.jphotobiol.2017.12.014. Epub 2017 Dec 15. PMID: 29275239. 2. Puc J, Kwiatkowski P, Pacanowski J, Rotbart-Fiedor M, Wardawa A, Mazurek AP, Rowinski W, Hardy MA, Fiedor P. Mitochondrial activity after cold preservation of pancreatic islet cells treated with pefloxacin (PFX). Ann Transplant. 1998;3(1):38-41. PMID: 9869897. 3. Skopkó BE, Deák Á, Matesz C, Kelentey B, Bácskai T. Pefloxacin induced changes in serotonergic innervation and mast cell number in rat salivary glands. Drug Chem Toxicol. 2020 Sep;43(5):496-503. doi: 10.1080/01480545.2018.1508217. Epub 2018 Sep 27. PMID: 30257570.

In vitro protocol:

In vivo protocol:

1. Skopkó BE, Deák Á, Matesz C, Kelentey B, Bácskai T. Pefloxacin induced changes in serotonergic innervation and mast cell number in rat salivary glands. Drug Chem Toxicol. 2020 Sep;43(5):496-503. doi: 10.1080/01480545.2018.1508217. Epub 2018 Sep 27. PMID: 30257570.

1: Bao T, Ke H, Li W, Cai L, Huang Y. Highly Efficient Peroxymonosulfate Electroactivation on Co(OH)2 Nanoarray Electrode for Pefloxacin Degradation. Nanomaterials (Basel). 2024 Aug 4;14(15):1312. doi: 10.3390/nano14151312. PMID: 39120417; PMCID: PMC11314119. 2: Guo Y, Li L, Xu S, Zhang M, Jiang C. Ion coordination and chelation in Eu- MOFs matrices: Ultrafast fluorescence visual quantification monitoring of antibiotic residues. Talanta. 2024 Oct 1;278:126549. doi: 10.1016/j.talanta.2024.126549. Epub 2024 Jul 14. PMID: 39018758. 3: Grace Oluwatoyin MA, Feyisara Banji A, Nevillah Nice A, Jennifer Orobosa E, Abosede Abolanle A, Seyi Samson E, Oluwatosin A. Bacterial diversity, antibiogram and nutritional assessment of cowhide (Ponmo) in Ilishan-Remo central market, Nigeria. Heliyon. 2024 May 8;10(10):e30882. doi: 10.1016/j.heliyon.2024.e30882. PMID: 38813194; PMCID: PMC11133659. 4: Zhou Y, Wang J. Electro-Fenton degradation of pefloxacin using MOFs derived Cu, N co-doped carbon as a nanocomposite catalyst. Environ Pollut. 2024 Aug 15;355:124198. doi: 10.1016/j.envpol.2024.124198. Epub 2024 May 21. PMID: 38782161. 5: Varghese G, Jamwal A, Deepika, Tejan N, Patel SS, Sahu C, Mishra S, Singh V. Trends in antimicrobial susceptibility pattern of Salmonella species isolated from bacteremia patients at a tertiary care center in Northern India. Diagn Microbiol Infect Dis. 2024 Aug;109(4):116354. doi: 10.1016/j.diagmicrobio.2024.116354. Epub 2024 May 15. PMID: 38776664. 6: Ruape WA, Mond C, Toroi W, Max B, Chanoan J, Duke T. Typhoid fever in children in Goroka, Papua New Guinea. J Paediatr Child Health. 2024 Jun;60(6):188-192. doi: 10.1111/jpc.16553. Epub 2024 Apr 25. PMID: 38661078. 7: Song Y, Sun X, Nghiem LD, Duan J, Liu W, Liu Y, Cai Z. Insight into Fe-O-Bi electron migration channel in MIL-53(Fe)/Bi4O5I2 Z-scheme heterojunction for efficient photocatalytic decontamination. J Colloid Interface Sci. 2024 Aug;667:321-337. doi: 10.1016/j.jcis.2024.04.096. Epub 2024 Apr 15. PMID: 38640652. 8: Wang C, Chang L, Zhang X, Chai H, Huang Y. Promoting oxygen vacancies utility for tetracycline degradation via peroxymonosulfate activation by reduced Mg- doped Co3O4: Kinetics and key role of electron transfer pathway. Environ Res. 2024 Jul 1;252(Pt 2):118892. doi: 10.1016/j.envres.2024.118892. Epub 2024 Apr 9. PMID: 38599451. 9: Zhang Y, He R, Sun Y, Zhao J, Zhang X, Wang J, Bildyukevich AV. Influence of microplastics and environmentally persistent free radicals on the ability of biochar components to promote degradation of antibiotics by activated peroxymonosulfate. Environ Pollut. 2024 May 15;349:123827. doi: 10.1016/j.envpol.2024.123827. Epub 2024 Apr 3. PMID: 38574947. 10: Zhang Y, Hu Y, Li X, Gao L, Wang S, Jia S, Shi P, Li A. Prevalence of antibiotics, antibiotic resistance genes, and their associations in municipal wastewater treatment plants along the Yangtze River basin, China. Environ Pollut. 2024 May 1;348:123800. doi: 10.1016/j.envpol.2024.123800. Epub 2024 Mar 20. PMID: 38518970. 11: Fan XF, Fu L, Cui GH. Three robust Cd(II) coordination polymers as bifunctional luminescent probes for efficient detection of pefloxacin and Cr2O72- in water. Dalton Trans. 2024 Mar 12;53(11):5051-5063. doi: 10.1039/d4dt00128a. PMID: 38375864. 12: Novak A, Dzelalija M, Goic-Barisic I, Kovacic A, Pirija M, Maravic A, Radic M, Marinovic J, Rubic Z, Carev M, Tonkic M. Phenotypic and Molecular Characterization of a Hospital Outbreak Clonal Lineage of Salmonella enterica Subspecies enterica serovar Mikawasima Containing blaTEM-1B and blaSHV-2 That Emerged on a Neonatal Ward, During the COVID-19 Pandemic. Microb Drug Resist. 2024 Mar;30(3):118-126. doi: 10.1089/mdr.2023.0132. Epub 2024 Feb 7. PMID: 38330414. 13: Chen H, Geng J, Shen J, Shi Q, Lv J, Lv Y, Song C. Synthesis and Catalytic Degradation of PEF, ENR, and CIP by g-C3N4/TCNQ/Eu Composite. Micromachines (Basel). 2023 Nov 24;14(12):2146. doi: 10.3390/mi14122146. PMID: 38138315; PMCID: PMC10745507. 14: Post HK, Blankespoor MG, Ierulli VK, Morey TD, Schroeppel JP, Mulcahey MK, Vopat BG, Vopat ML. Review of Intra-Articular Use of Antibiotics and Antiseptic Irrigation and Their Systematic Association with Chondrolysis. Kans J Med. 2023 Oct 30;16:272-276. doi: 10.17161/kjm.vol16.20357. PMID: 37954883; PMCID: PMC10635690. 15: Li G, Qi X, Wu J, Wan X, Wang T, Liu Y, Chen Y, Xia Y. Highly stable electrochemical sensing platform for the selective determination of pefloxacin in food samples based on a molecularly imprinted-polymer-coated gold nanoparticle/black phosphorus nanocomposite. Food Chem. 2024 Mar 15;436:137753. doi: 10.1016/j.foodchem.2023.137753. Epub 2023 Oct 15. PMID: 37862994. 16: Beig M, Moradkasani S, Goodarzi F, Sholeh M. Prevalence of Brucella melitensis and Brucella abortus Fluoroquinolones Resistant Isolates: A Systematic Review and Meta-Analysis. Vector Borne Zoonotic Dis. 2024 Jan;24(1):1-9. doi: 10.1089/vbz.2023.0063. Epub 2023 Oct 20. PMID: 37862228. 17: Braga PRC, Dos Santos CA, Bertani AMJ, Vieira T, Amarante AF, Reis AD, Sacchi CT, Camargo CH, Ribeiro MG, Borges AS, Tiba-Casas MR. Detection and genomic characterization of a multidrug-resistant Salmonella Newport co- harbouring blaCMY-2, qnrB19 and mcr-9 from the diarrheic faeces of a foal. J Glob Antimicrob Resist. 2023 Dec;35:198-201. doi: 10.1016/j.jgar.2023.09.019. Epub 2023 Oct 5. PMID: 37805072. 18: Konyali D, Guzel M, Soyer Y. Genomic Characterization of Salmonella enterica Resistant to Cephalosporin, Quinolones, And Macrolides. Curr Microbiol. 2023 Sep 19;80(11):344. doi: 10.1007/s00284-023-03458-y. PMID: 37725171. 19: Vergara-Luis I, Bocayá N, Irazola-Duñabeitia M, Zuloaga O, Lacuesta M, Olivares M, Prieto A. Multitarget and suspect screening of antimicrobials in soil and manure by means of QuEChERS - liquid chromatography tandem mass spectrometry. Anal Bioanal Chem. 2023 Oct;415(25):6291-6310. doi: 10.1007/s00216-023-04905-2. Epub 2023 Aug 23. PMID: 37610438; PMCID: PMC10558387. 20: Ahmed S, Mahendiran D, Bhat AR, Rahiman AK. Theoretical, in Vitro Antiproliferative, and in Silico Molecular Docking and Pharmacokinetics Studies of Heteroleptic Nickel(II) and Copper(II) Complexes of Thiosemicarbazone-Based Ligands and Pefloxacin. Chem Biodivers. 2023 Sep;20(9):e202300702. doi: 10.1002/cbdv.202300702. Epub 2023 Aug 14. PMID: 37528701.