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MedKoo Cat#: 584395 | Name: Pefloxacin mesylate
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Pefloxacin is a synthetic broad-spectrum fluoroquinolone antibacterial agent active against most gram-negative and gram-positive bacteria. Pefloxacin mesylate is used to create a new type of dual-band fluorescence biosensor for high-efficiency and sensitive determination of double-stranded DNA

Chemical Structure

Pefloxacin mesylate
Pefloxacin mesylate
CAS#70458-95-6 (mesylate)

Theoretical Analysis

MedKoo Cat#: 584395

Name: Pefloxacin mesylate

CAS#: 70458-95-6 (mesylate)

Chemical Formula: C18H24FN3O6S

Exact Mass: 333.1489

Molecular Weight: 429.46

Elemental Analysis: C, 50.34; H, 5.63; F, 4.42; N, 9.78; O, 22.35; S, 7.47

Price and Availability

Size Price Availability Quantity
100mg USD 350.00 2 Weeks
500mg USD 550.00 2 Weeks
1g USD 950.00 2 Weeks
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Related CAS #
70458-95-6 (mesylate) 70458-92-3 (free base)
Synonym
Pefloxacin; 1584RB; 1589 RB; EU 5306; EU-5306; Pefloxacin mesylate
IUPAC/Chemical Name
3-Quinolinecarboxylic acid, 1,4-dihydro-1-ethyl-6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-, mesylate
InChi Key
HQQSBEDKMRHYME-UHFFFAOYSA-N
InChi Code
InChI=1S/C17H20FN3O3.CH4O3S/c1-3-20-10-12(17(23)24)16(22)11-8-13(18)15(9-14(11)20)21-6-4-19(2)5-7-21;1-5(2,3)4/h8-10H,3-7H2,1-2H3,(H,23,24);1H3,(H,2,3,4)
SMILES Code
O=C(C1=CN(CC)C2=C(C=C(F)C(N3CCN(C)CC3)=C2)C1=O)O.OS(=O)(C)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Pefloxacin mesylate is a an antibacterial agent and prevents bacterial DNA replication by inhibiting DNA gyrase (topoisomerse).
In vitro activity:
PFLX (pefloxacin) was photodegraded in 5h and forms photoproduct under UVA exposure. At the non photocytotoxic dose PFLX, shows reduced phagocytosis activity, NO (nitric oxide) production, large vacuole formation and down regulated IL-6, TNF-α and IL-1 in BALB/c macrophages at both genes and proteins levels. At higher doses (photocytotoxic doses), PFLX induced a concentration dependent decrease in cell viability of human keratinocyte cell line (HaCaT) and peritoneal macrophages of BALB/c mice. Reference: J Photochem Photobiol B. 2018 Jan;178:593-605. https://pubmed.ncbi.nlm.nih.gov/29275239/
In vivo activity:
The aim of this study was to investigate the changes in the number of serotonergic immunoreactive fibers and mast cells after pefloxacin treatment in the parotid and sublingual glands of rats to detect the possible neurotoxic effect of pefloxacin. These results suggest that pefloxacin treatment can modify the finely controlled communication between the immune- and the peripheral nervous systems, resulting neurogenic inflammatory process. Reference: Drug Chem Toxicol. 2020 Sep;43(5):496-503. https://pubmed.ncbi.nlm.nih.gov/30257570/
Solvent mg/mL mM
Solubility
DMSO 13.3 30.85
Water 83.5 194.43
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 429.46 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Singh J, Srivastva AK, Mandal P, Chandra S, Dubey D, Dwivedi A, Chopra D, Tripathi A, Ray RS. Under ambient UVA exposure, pefloxacin exhibits both immunomodulatory and genotoxic effects via multiple mechanisms. J Photochem Photobiol B. 2018 Jan;178:593-605. doi: 10.1016/j.jphotobiol.2017.12.014. Epub 2017 Dec 15. PMID: 29275239. 2. Puc J, Kwiatkowski P, Pacanowski J, Rotbart-Fiedor M, Wardawa A, Mazurek AP, Rowinski W, Hardy MA, Fiedor P. Mitochondrial activity after cold preservation of pancreatic islet cells treated with pefloxacin (PFX). Ann Transplant. 1998;3(1):38-41. PMID: 9869897. 3. Skopkó BE, Deák Á, Matesz C, Kelentey B, Bácskai T. Pefloxacin induced changes in serotonergic innervation and mast cell number in rat salivary glands. Drug Chem Toxicol. 2020 Sep;43(5):496-503. doi: 10.1080/01480545.2018.1508217. Epub 2018 Sep 27. PMID: 30257570.
In vitro protocol:
1. Singh J, Srivastva AK, Mandal P, Chandra S, Dubey D, Dwivedi A, Chopra D, Tripathi A, Ray RS. Under ambient UVA exposure, pefloxacin exhibits both immunomodulatory and genotoxic effects via multiple mechanisms. J Photochem Photobiol B. 2018 Jan;178:593-605. doi: 10.1016/j.jphotobiol.2017.12.014. Epub 2017 Dec 15. PMID: 29275239. 2. Puc J, Kwiatkowski P, Pacanowski J, Rotbart-Fiedor M, Wardawa A, Mazurek AP, Rowinski W, Hardy MA, Fiedor P. Mitochondrial activity after cold preservation of pancreatic islet cells treated with pefloxacin (PFX). Ann Transplant. 1998;3(1):38-41. PMID: 9869897.
In vivo protocol:
1. Skopkó BE, Deák Á, Matesz C, Kelentey B, Bácskai T. Pefloxacin induced changes in serotonergic innervation and mast cell number in rat salivary glands. Drug Chem Toxicol. 2020 Sep;43(5):496-503. doi: 10.1080/01480545.2018.1508217. Epub 2018 Sep 27. PMID: 30257570.
1: Zhang B, Lang Y, Guo B, Cao Z, Cheng J, Cai D, Shentu X, Yu X. Indirect Competitive Enzyme-Linked Immunosorbent Assay Based on Broad-Spectrum Antibody for Simultaneous Determination of Thirteen Fluoroquinolone Antibiotics in Rana catesbeianus. Foods. 2023 Jun 29;12(13):2530. doi: 10.3390/foods12132530. PMID: 37444268; PMCID: PMC10340495. 2: Feng J, Jiang H, Wang J, Jing Z, Zhang F, Tan T, He F, Jiang L, Li H, Chang S, Li T. [Simultaneous determination of 40 plant growth regulators, fungicides, insecticides, and antibiotics in bean sprouts by QuEChERS-high performance liquid chromatography-tandem mass spectrometry]. Se Pu. 2022 Sep;40(9):843-853. Chinese. doi: 10.3724/SP.J.1123.2021.12028. PMID: 36156631; PMCID: PMC9520376. 3: Zhang Q, Duan S, Huang Y, Tian J, Hu J. Dual-band fluorescence detection of double-stranded DNA with QDs-Mn2+-pefloxacin. Colloids Surf B Biointerfaces. 2022 Sep;217:112649. doi: 10.1016/j.colsurfb.2022.112649. Epub 2022 Jun 20. PMID: 35753193. 4: Zhu G, Cheng G, Wang P, Li W, Wang Y, Fan J. Water compatible imprinted polymer prepared in water for selective solid phase extraction and determination of ciprofloxacin in real samples. Talanta. 2019 Aug 1;200:307-315. doi: 10.1016/j.talanta.2019.03.070. Epub 2019 Mar 16. PMID: 31036189. 5: Lin S, Li G, Zhao Z, Fu J, Feng M, Song M, Huang Z, Yang S, Wang S, Wan R. Pharmacokinetics and tissue distribution of pefloxacin mesylate in chickens. Biomed Chromatogr. 2018 Apr;32(4). doi: 10.1002/bmc.4154. Epub 2017 Dec 26. PMID: 29178369. 6: Sharma C, Biswas NR, Ojha S, Velpandian T. Comprehensive evaluation of formulation factors for ocular penetration of fluoroquinolones in rabbits using cassette dosing technique. Drug Des Devel Ther. 2016 Feb 22;10:811-23. doi: 10.2147/DDDT.S95870. PMID: 26955263; PMCID: PMC4769009. 7: Li A, Song Z. Study of silver nanoparticles sensitized fluorescence and second-order scattering of terbium(III)-pefloxacin mesylate complex and determination of pefloxacin mesylate. ScientificWorldJournal. 2014;2014:742935. doi: 10.1155/2014/742935. Epub 2014 May 6. PMID: 24892083; PMCID: PMC4032712. 8: Guo M, Lu XW, Ran XY, Hu RH. [Molecular transport mechanism of pefloxacin mesylate binding with transferrin]. Yao Xue Xue Bao. 2012 Nov;47(11):1503-10. Chinese. PMID: 23387084. 9: Liu R, Li N, Liu N, Zhou X, Dong ZM, Wen XJ, Liu LC. Effects of systemic ornidazole, systemic and local compound ornidazole and pefloxacin mesylate on experimental periodontitis in rats. Med Sci Monit. 2012 Mar;18(3):BR95-102. doi: 10.12659/msm.882514. PMID: 22367122; PMCID: PMC3560749. 10: Liu Y, Huang Y, Liu J, Wang W, Liu G, Zhao R. Superparamagnetic surface molecularly imprinted nanoparticles for water-soluble pefloxacin mesylate prepared via surface initiated atom transfer radical polymerization and its application in egg sample analysis. J Chromatogr A. 2012 Jul 13;1246:15-21. doi: 10.1016/j.chroma.2012.01.045. Epub 2012 Jan 25. PMID: 22321951. 11: Olcay E, Beytemur O, Kaleagasioglu F, Gulmez T, Mutlu Z, Olgac V. Oral toxicity of pefloxacin, norfloxacin, ofloxacin and ciprofloxacin: comparison of biomechanical and histopathological effects on Achilles tendon in rats. J Toxicol Sci. 2011 Jun;36(3):339-45. doi: 10.2131/jts.36.339. PMID: 21628961. 12: Xie Y, Song Y, Zhang Y, Zhao B. Near-infrared spectroscopy quantitative determination of pefloxacin mesylate concentration in pharmaceuticals by using partial least squares and principal component regression multivariate calibration. Spectrochim Acta A Mol Biomol Spectrosc. 2010 May;75(5):1535-9. doi: 10.1016/j.saa.2010.02.012. Epub 2010 Feb 21. PMID: 20299275. 13: Deng B, Li L, Shi A, Kang Y. Pharmacokinetics of pefloxacin mesylate in human urine using capillary electrophoresis electrochemiluminescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 15;877(24):2585-8. doi: 10.1016/j.jchromb.2009.06.021. Epub 2009 Jun 21. PMID: 19576860. 14: Goudah A, Shah SS, Shin HC, Chang BJ, Shim JH, Abd El-Aty AM. Concentration- time courses of pefloxacin in plasma and milk of lactating she-camels (Camelus dromedarius). Berl Munch Tierarztl Wochenschr. 2008 Nov-Dec;121(11-12):432-9. PMID: 19086693. 15: Asli B, Bienvenu B, Cordoliani F, Brouet JC, Uzunhan Y, Arnulf B, Malphettes M, Rybojad M, Fermand JP. Chronic urticaria and monoclonal IgM gammopathy (Schnitzler syndrome): report of 11 cases treated with pefloxacin. Arch Dermatol. 2007 Aug;143(8):1046-50. doi: 10.1001/archderm.143.8.1046. PMID: 17709664. 16: Basavaiah K, Prameela HC, Somashekar BC. Spectrophotometric determination of pefloxacin mesylate in pharmaceuticals. Acta Pharm. 2007 Jun;57(2):221-30. doi: 10.2478/v10007-007-0018-4. PMID: 17507318. 17: Sultana Y, Aqil M, Ali A, Zafar S. Evaluation of carbopol-methyl cellulose based sustained-release ocular delivery system for pefloxacin mesylate using rabbit eye model. Pharm Dev Technol. 2006;11(3):313-9. doi: 10.1080/10837450600767698. PMID: 16895842. 18: Fan JC, Chen X, Wang Y, Fan CP, Shang ZC. Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin. J Zhejiang Univ Sci B. 2006 Jun;7(6):452-8. doi: 10.1631/jzus.2006.B0452. PMID: 16691639; PMCID: PMC1473993. 19: Sultana Y, Aqil M, Ali A. Ion-activated, Gelrite-based in situ ophthalmic gels of pefloxacin mesylate: comparison with conventional eye drops. Drug Deliv. 2006 May-Jun;13(3):215-9. doi: 10.1080/10717540500309164. PMID: 16556574. 20: Sultana Y, Aqil M, Ali A. Ocular inserts for controlled delivery of pefloxacin mesylate: preparation and evaluation. Acta Pharm. 2005 Sep;55(3):305-14. PMID: 16375841.