Influenza Hemagglutinin Peptide | CAS#92000-76-5 | Hemagglutinin HA1 peptide

MedKoo Cat#: 463888 | Name: Influenza Hemagglutinin Peptide

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Influenza hemagglutinin (HA) peptide is a peptide from the hemagglutinin HA1 chain of influenza virus that corresponds to amino acids 98-106 of the full-length protein. Due to its small size, low potential to interfere in protein folding or activity, and strong immunoreactivity, it is commonly used as an epitope tag on recombinant proteins to facilitate their detection and purification.

Chemical Structure

Influenza Hemagglutinin Peptide

CAS#92000-76-5

Theoretical Analysis

MedKoo Cat#: 463888

Name: Influenza Hemagglutinin Peptide

CAS#: 92000-76-5

Chemical Formula: C55H68F3N9O19

Exact Mass:

Molecular Weight: 1216.19

Elemental Analysis: C, 54.32; H, 5.64; F, 4.69; N, 10.37; O, 24.99

Price and Availability

Size	Price	Availability	Quantity
1mg	USD 265.00	2 Weeks
5mg	USD 650.00	2 Weeks

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Synonym

Influenza Hemagglutinin Peptide; YPYDVPDYA;

IUPAC/Chemical Name

(S)-3-((S)-2-((S)-1-(L-tyrosyl)pyrrolidine-2-carboxamido)-3-(4-hydroxyphenyl)propanamido)-4-(((S)-1-((S)-2-(((S)-3-carboxy-1-(((S)-1-(((S)-1-carboxyethyl)amino)-3-(4-hydroxyphenyl)-1-oxopropan-2-yl)amino)-1-oxopropan-2-yl)carbamoyl)pyrrolidin-1-yl)-3-methyl-1-oxobutan-2-yl)amino)-4-oxobutanoic acid compound with 2,2,2-trifluoroacetic acid (1:1)

InChi Key

PQLXMPRGFNZLLO-KUMJZVTDSA-N

InChi Code

InChI=1S/C53H67N9O17.C2HF3O2/c1-27(2)44(52(77)62-21-5-7-41(62)50(75)59-38(25-42(66)67)47(72)56-36(45(70)55-28(3)53(78)79)23-30-10-16-33(64)17-11-30)60-48(73)39(26-43(68)69)57-46(71)37(24-31-12-18-34(65)19-13-31)58-49(74)40-6-4-20-61(40)51(76)35(54)22-29-8-14-32(63)15-9-29;3-2(4,5)1(6)7/h8-19,27-28,35-41,44,63-65H,4-7,20-26,54H2,1-3H3,(H,55,70)(H,56,72)(H,57,71)(H,58,74)(H,59,75)(H,60,73)(H,66,67)(H,68,69)(H,78,79);(H,6,7)/t28-,35-,36-,37-,38-,39-,40-,41-,44-;/m0./s1

SMILES Code

OC1=CC=C(C[C@@H](C(N[C@H](C(N[C@H](C(N2CCC[C@H]2C(N[C@H](C(N[C@H](C(N[C@H](C(O)=O)C)=O)CC3=CC=C(C=C3)O)=O)CC(O)=O)=O)=O)C(C)C)=O)CC(O)=O)=O)NC([C@H]4N(CCC4)C([C@H](CC5=CC=C(C=C5)O)N)=O)=O)C=C1.OC(C(F)(F)F)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). HA Peptide is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.

In vitro activity:

Finally, four mAbs against HA antigen of H1N1 influenza virus were obtained. The results of ELISA and computer prediction showed that there were at least two types of epitopes on HA of influenza virus. The results of this study complemented the existing methods for predicting HA epitopes, and also provided a new method for predicting other pathogenic microorganisms. Reference: Arch Microbiol. 2022 Sep 2;204(9):594. https://pubmed.ncbi.nlm.nih.gov/36053375/

In vivo activity:

Recombinant L. plantarum expressed swIAV HA1 protein (pSIP401-HA1-ZN-3) and conferred effective mucosal, cellular and systemic immune responses in the intestine as well as in the upper respiratory airways of mice. In conclusion, the oral and intranasal administration of L. plantarum strain pSIP401-HA1-ZN-3 in mice induced mucosal immunity and most importantly, provided protection against lethal influenza virus challenge. Reference: Microb Cell Fact. 2022 Sep 9;21(1):185. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9461438/

	Solvent	mg/mL	mM
Solubility
	Water	50.0	41.11

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 1,216.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Feng Q, Huang XY, Feng YM, Sun LJ, Sun JY, Li Y, Xie X, Hu J, Guo CY. Identification and analysis of B cell epitopes of hemagglutinin of H1N1 influenza virus. Arch Microbiol. 2022 Sep 2;204(9):594. doi: 10.1007/s00203-022-03133-z. PMID: 36053375; PMCID: PMC9438888. 2. Garcia-Moro E, Zhang J, Calder LJ, Brown NR, Gamblin SJ, Skehel JJ, Rosenthal PB. Reversible structural changes in the influenza hemagglutinin precursor at membrane fusion pH. Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2208011119. doi: 10.1073/pnas.2208011119. Epub 2022 Aug 8. PMID: 35939703; PMCID: PMC9388137. 3. Zhang Y, Yang L, Zhang J, Huang K, Sun X, Yang Y, Wang T, Zhang Q, Zou Z, Jin M. Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus. Microb Cell Fact. 2022 Sep 9;21(1):185. doi: 10.1186/s12934-022-01911-4. PMID: 36085207; PMCID: PMC9461438. 4. Zhang L, Chen J, Shen C, Wang G, Lu Z, Zeng D, Gao Y, Chen H, Xia N, Chen Y. Vaccination with Deglycosylated Modified Hemagglutinin Broadly Protects against Influenza Virus Infection in Mice and Ferrets. Vaccines (Basel). 2022 Aug 11;10(8):1304. doi: 10.3390/vaccines10081304. PMID: 36016191; PMCID: PMC9414581.

In vitro protocol:

In vivo protocol:

1. Zhang Y, Yang L, Zhang J, Huang K, Sun X, Yang Y, Wang T, Zhang Q, Zou Z, Jin M. Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus. Microb Cell Fact. 2022 Sep 9;21(1):185. doi: 10.1186/s12934-022-01911-4. PMID: 36085207; PMCID: PMC9461438. 2. Zhang L, Chen J, Shen C, Wang G, Lu Z, Zeng D, Gao Y, Chen H, Xia N, Chen Y. Vaccination with Deglycosylated Modified Hemagglutinin Broadly Protects against Influenza Virus Infection in Mice and Ferrets. Vaccines (Basel). 2022 Aug 11;10(8):1304. doi: 10.3390/vaccines10081304. PMID: 36016191; PMCID: PMC9414581.

1: Du R, Cheng H, Cui Q, Peet NP, Gaisina IN, Rong L. Identification of a novel inhibitor targeting influenza A virus group 2 hemagglutinins. Antiviral Res. 2021 Jan 8;186:105013. doi: 10.1016/j.antiviral.2021.105013. Epub ahead of print. PMID: 33428962. 2: Wang Q, Sun Z, Li J, Qin T, Ma H, Chen S, Peng D, Liu X. Identification of a universal antigen epitope of influenza A virus using peptide microarray. BMC Vet Res. 2021 Jan 7;17(1):22. doi: 10.1186/s12917-020-02725-5. PMID: 33413356; PMCID: PMC7792037. 3: Liting Y, Lijun S, Chunyan G, Lanlan L, Jingying S, Xiaoyan H, Penghua Z, Xin X, Jun H. Neutralizing antibody PR8-23 targets the footprint of the sialoglycan receptor binding site of H1N1 hemagglutinin. J Med Virol. 2021 Jan 7. doi: 10.1002/jmv.26779. Epub ahead of print. PMID: 33410516. 4: Do PC, Nguyen TH, Vo UHM, Le L. iBRAB: In silico based-designed broad- spectrum Fab against H1N1 influenza A virus. PLoS One. 2020 Dec 31;15(12):e0239112. doi: 10.1371/journal.pone.0239112. PMID: 33382708; PMCID: PMC7774956. 5: Takenaka T, Sakamoto W, Takahashi S, Shimada N, Maruyama A. Spatially regulated activation of membrane fusogenic peptides with chaperone-like ionic copolymers. J Control Release. 2020 Dec 25;330:463-469. doi: 10.1016/j.jconrel.2020.12.030. Epub ahead of print. PMID: 33359738. 6: Khrustalev VV, Kordyukova LV, Arutyunyan AM, Poboinev VV, Khrustaleva TA, Stojarov AN, Baratova LA, Sapon AS, Lugin VG. The cytoplasmic tail of influenza A/H1N1 virus hemagglutinin is β-structural. J Biomol Struct Dyn. 2020 Dec 15:1-20. doi: 10.1080/07391102.2020.1860827. Epub ahead of print. PMID: 33317396. 7: Bimler L, Ronzulli SL, Song AY, Johnson SK, Jones CA, Kim T, Le DT, Tompkins SM, Paust S. Matrix Protein 2 Extracellular Domain-Specific Monoclonal Antibodies Are an Effective and Potentially Universal Treatment for Influenza A. J Virol. 2020 Dec 2:JVI.01027-20. doi: 10.1128/JVI.01027-20. Epub ahead of print. PMID: 33268521. 8: Gao J, Gui M, Xiang Y. Structural intermediates in the low pH-induced transition of influenza hemagglutinin. PLoS Pathog. 2020 Nov 30;16(11):e1009062. doi: 10.1371/journal.ppat.1009062. PMID: 33253316; PMCID: PMC7728236. 9: Islam MSB, Miah M, Hossain ME, Kibria KMK. A conserved multi-epitope-based vaccine designed by targeting hemagglutinin protein of highly pathogenic avian H5 influenza viruses. 3 Biotech. 2020 Dec;10(12):546. doi: 10.1007/s13205-020-02544-3. Epub 2020 Nov 23. PMID: 33251084; PMCID: PMC7682764. 10: Lousa D, Pinto ART, Campos SRR, Baptista AM, Veiga AS, Castanho MARB, Soares CM. Effect of pH on the influenza fusion peptide properties unveiled by constant-pH molecular dynamics simulations combined with experiment. Sci Rep. 2020 Nov 18;10(1):20082. doi: 10.1038/s41598-020-77040-y. PMID: 33208852; PMCID: PMC7674464. 11: Bashashati M, Mojahedi Z, Roudsari AA, Taghizadeh M, Molouki A, Motamed N, Sabouri F, Fallah Mehrabadi MH. Ongoing genetic evolution of H9N2 avian influenza viruses in Iranian industrial poultry farms. Acta Vet Hung. 2020 Nov 12. doi: 10.1556/004.2020.00048. Epub ahead of print. PMID: 33185568. 12: Khrustalev VV. The PentaFOLD 3.0 algorithm for the selection of stable elements of secondary structure to be included in vaccine peptides. Protein Pept Lett. 2020 Nov 10. doi: 10.2174/0929866527666201110123851. Epub ahead of print. PMID: 33172366. 13: Peubez I, Margot S, Buffin S, Pion C, Bernard MC, Dinadayala P, Poncet D, Deloire S, Marco S, Legastelois I. A novel lamprey antibody sequence to multimerize and increase the immunogenicity of recombinant viral and bacterial vaccine antigens. Vaccine. 2020 Nov 25;38(50):7905-7915. doi: 10.1016/j.vaccine.2020.10.073. Epub 2020 Nov 2. PMID: 33153770. 14: Jiang W, Pilkington EH, Kelly HG, Tan HX, Juno JA, Wheatley AK, Kent SJ. Aggregation by peptide conjugation rescues poor immunogenicity of the HA stem. PLoS One. 2020 Nov 2;15(11):e0241649. doi: 10.1371/journal.pone.0241649. PMID: 33137148; PMCID: PMC7605677. 15: Pon R, Marcil A, Chen W, Gadoury C, Williams D, Chan K, Zhou H, Ponce A, Paquet E, Gurnani K, Chattopadhyay A, Zou W. Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies. Sci Rep. 2020 Oct 28;10(1):18497. doi: 10.1038/s41598-020-75754-7. PMID: 33116268; PMCID: PMC7595224. 16: Burke DF, Mantell RG, Pitt CE, Wales DJ. Energy Landscape for the Membrane Fusion Pathway in Influenza A Hemagglutinin From Discrete Path Sampling. Front Chem. 2020 Sep 25;8:575195. doi: 10.3389/fchem.2020.575195. PMID: 33102445; PMCID: PMC7546250. 17: Kajiwara N, Nomura N, Ukaji M, Yamamoto N, Kohara M, Yasui F, Sakoda Y, Kida H, Shibasaki F. Cell-penetrating peptide-mediated cell entry of H5N1 highly pathogenic avian influenza virus. Sci Rep. 2020 Oct 22;10(1):18008. doi: 10.1038/s41598-020-74604-w. PMID: 33093460; PMCID: PMC7582914. 18: Dang X, Wang G. Spotlight on the Selected New Antimicrobial Innate Immune Peptides Discovered During 2015-2019. Curr Top Med Chem. 2020;20(32):2984-2998. doi: 10.2174/1568026620666201022143625. PMID: 33092508. 19: Li S, Qiao Y, Xu Y, Li P, Nie J, Zhao Q, Chai W, Shi Y, Kong W, Shan Y. Identification of Linear Peptide Immunogens with Verified Broad-spectrum Immunogenicity from the Conserved Regions within the Hemagglutinin Stem Domain of H1N1 Influenza Virus. Immunol Invest. 2020 Oct 20:1-14. doi: 10.1080/08820139.2020.1834579. Epub ahead of print. PMID: 33078652. 20: Guo Y, Hu Y, Zheng X, Cao X, Li Q, Wei Z, Zhu Z, Zhang S. Self-assembled peptide nanoparticles with endosome escaping permits for co-drug delivery. Talanta. 2021 Jan 1;221:121572. doi: 10.1016/j.talanta.2020.121572. Epub 2020 Sep 6. PMID: 33076119.