Synonym
C.I. Basic Blue 8; Victoria Blue 4R; Basic Blue 8; Basonyl Blue 633; C.I. 42563; Victoria Basic Blue F 4R; Victoria Blue F 4R;
IUPAC/Chemical Name
(4-((4-(Dimethylamino)phenyl)(4-toluidino-1-naphthyl)methylene)cyclohexa-2,5-dien-1-ylidene)dimethylammonium chloride
InChi Key
HJSOFCVADRYENQ-UHFFFAOYSA-N
InChi Code
InChI=1S/C34H33N3.ClH/c1-24-10-17-28(18-11-24)35-32-23-16-25-8-6-7-9-31(25)34(32)33(26-12-19-29(20-13-26)36(2)3)27-14-21-30(22-15-27)37(4)5;/h6-23H,1-5H3;1H
SMILES Code
C/[N+](C)=C1C=C/C(C=C\1)=C(C2=CC=C(N(C)C)C=C2)/C3=C4C=CC=CC4=CC=C3NC5=CC=C(C)C=C5.[Cl-]
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
520.12
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Luo Y, Wang X, Liu Q, Liang A, He X, Jiang Z. A sensitive surface-enhanced Raman scattering method for chondroitin sulfate with Victoria blue 4R molecular probes in nanogold sol substrate. Luminescence. 2018 Feb;33(1):131-137. doi: 10.1002/bio.3382. Epub 2017 Aug 11. PMID: 28799722.
2: Wohlrab F, von Dorsche HH, Krautschick I, Schmidt S. On the specificity of insulin staining by Victoria blue 4R. Histochem J. 1985 May;17(5):515-8. doi: 10.1007/BF01003177. PMID: 3897146.
3: Wen G, Liang X, Liu Q, Liang A, Jiang Z. A novel nanocatalytic SERS detection of trace human chorionic gonadotropin using labeled-free Vitoria blue 4R as molecular probe. Biosens Bioelectron. 2016 Nov 15;85:450-456. doi: 10.1016/j.bios.2016.05.024. Epub 2016 May 7. PMID: 27208477.
4: Li C, Wang X, Liang A, Luo Y, Wen G, Jiang Z. A simple gold nanoplasmonic SERS method for trace Hg2+ based on aptamer-regulating graphene oxide catalysis. Luminescence. 2018 Sep;33(6):1113-1121. doi: 10.1002/bio.3517. Epub 2018 Jul 17. PMID: 30014561.
5: Teague O. The Toxicity of Victoria Blue 4R for B. paratyphosus A, B. paratyphosus B and B. enteritidis. J Bacteriol. 1918 Jan;3(1):1-6. PMID: 16558772; PMCID: PMC378737.
6: KATO K. Peroxidase staining and Victoria blue 4R staining of animal basophil leucocytes; the 25th report of histochemical study of peroxidase. Okajimas Folia Anat Jpn. 1957 Mar;29(6):381-6. doi: 10.2535/ofaj1936.29.6_381. PMID: 13431170.
7: Wohlrab F, Schwarz J. Spektrophotometrische Untersuchungen zur Bindung von Viktoriablau 4R an oxidiertes Insulin [Spectrophotometric studies on the binding of Victoria Blue 4R to oxidized insulin]. Acta Histochem. 1988;84(2):187-94. German. PMID: 3149439.
8: HERZBERG K. [The technic of staining elementary bodies of variola and vaccinia virus with Victoria blue 4R]. Zentralbl Bakteriol. 1960 Jan;177:145-8. German. PMID: 13852299.
9: Wang X, Jiang C, Qin Y, Peng Y, Wen G, Liang A, Jiang Z. SERS spectral study of HAuCl4-cysteine nanocatalytic reaction and its application for detection of heparin sodium with label-free VB4r molecular probe. Sci Rep. 2017 Apr 5;7:45979. doi: 10.1038/srep45979. PMID: 28378828; PMCID: PMC5380991.
10: Liang A, Li X, Zhang X, Wen G, Jiang Z. A sensitive SERS quantitative analysis method for Ni2+ by the dimethylglyoxime reaction regulating a graphene oxide nanoribbon catalytic gold nanoreaction. Luminescence. 2018 Sep;33(6):1033-1039. doi: 10.1002/bio.3504. Epub 2018 Jun 13. PMID: 29900660.
11: Duerden BI, Collee JG, Brown R, Deacon AG, Holbrook WP. A scheme for the identification of clinical isolates of Gram-negative anaerobic bacilli by conventional bacteriological tests. J Med Microbiol. 1980 May;13(2):231-45. doi: 10.1099/00222615-13-2-231. PMID: 6103963.
12: Liu S, Luo H, Li N, Liu Z, Zheng W. Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes. Anal Chem. 2001 Aug 15;73(16):3907-14. doi: 10.1021/ac001454h. PMID: 11534715.
13: Deugnier Y, Ramée MP, Delamaire D, Bourel M, Ferrand B. Détection histochimique de l'antigène HBs intrahépatocytaire par la coloration au bleu Victoria [Victoria blue staining for intrahepatocytic HBs Ag detection (author's transl)]. Gastroenterol Clin Biol. 1981 Oct;5(10):853-6. French. PMID: 6170536.
14: Marshall PN. Thin-layer chromatography of some cationic dyes commonly used in histology. J Chromatogr. 1976 Dec 22;129:277-85. doi: 10.1016/s0021-9673(00)87785-7. PMID: 63467.
15: Dutt MK. Use of aqueous solutions of two basic dyes for the demonstration of DNA. Microsc Acta. 1982 Mar;85(4):381-6. PMID: 6175884.
