RAMB4 | PTP1B-IN-9 | CAS#145888-79-5 | PTP1B inhibitor

MedKoo Cat#: 408101 | Name: RAMB4

Description:

WARNING: This product is for research use only, not for human or veterinary use.

RAMB4, also known as PTP1B-IN-9, is a ubiquitin-proteasome system stressor. RAMB4 selectively reduces the viability of cervical cancer cells independently of HPV genotype via blockade of proteasomal degradation. RAMB4 treatment triggers a Ubiquitin-Proteasome-System-stress response without affecting 20S proteasome catalytic activities.

Chemical Structure

RAMB4

CAS#145888-79-5

Theoretical Analysis

MedKoo Cat#: 408101

Name: RAMB4

CAS#: 145888-79-5

Chemical Formula: C19H13Cl4NO

Exact Mass: 410.9751

Molecular Weight: 413.12

Elemental Analysis: C, 55.24; H, 3.17; Cl, 34.32; N, 3.39; O, 3.87

Price and Availability

Size	Price	Availability
100mg	USD 450.00	2 Weeks
200mg	USD 750.00	2 Weeks
500mg	USD 1,250.00	2 Weeks
1g	USD 1,850.00	2 Weeks
2g	USD 3,250.00	2 Weeks

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Related CAS #

No Data

Synonym

PTP1B-IN-9; PTP1B-IN 9; PTP1B-IN9; RAMB4; RAMB-4; RAMB 4;

IUPAC/Chemical Name

(3E,5E)-3,5-Bis(3,4-dichlorobenzylidene)piperidin-4-one

InChi Key

GJPXGFGIFQWUOC-ACFHMISVSA-N

InChi Code

InChI=1S/C19H13Cl4NO/c20-15-3-1-11(7-17(15)22)5-13-9-24-10-14(19(13)25)6-12-2-4-16(21)18(23)8-12/h1-8,24H,9-10H2/b13-5+,14-6+

SMILES Code

O=C1/C(CNC/C1=C\C2=CC=C(Cl)C(Cl)=C2)=C/C3=CC=C(Cl)C(Cl)=C3

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Cervical cancer cells exhibit an increased requirement for ubiquitin-dependent protein degradation associated with an elevated metabolic turnover rate, and for specific signaling pathways, notably HPV E6-targeted degradation of p53 and PDZ proteins. Natural compounds with antioxidant properties including flavonoids and triterpenoids hold promise as anticancer agents by interfering with ubiquitin-dependent protein degradation. An increasing body of evidence indicates that their α-β unsaturated carbonyl system is the molecular determinant for inhibition of ubiquitin-mediated protein degradation up-stream of the catalytic sites of the 20S proteasome.

Product Data

Product Data

Instruction

View handling instruction

Biological target:

RAMB4 is an inhibitor of the ubiquitin-proteasome system. It increases the levels of polyubiquitinated proteins in HeLa cervical cancer cells, but does not inhibit the chymotrypsin-like, trypsin-like, or peptidylglutamyl peptide hydrolyzing-like activities of the 20S proteasome.

In vitro activity:

RAMB1 treatment results in activation of lysosomal-dependent degradation pathways as a mechanism to compensate for increasing levels of poly-ubiquitin enriched toxic aggregates. RAMB1 synergistically triggers cell death of cervical cancer cells when combined with Chloroquine. Reference: PLoS One. 2011;6(8):e23888. https://pubmed.ncbi.nlm.nih.gov/21909374/

In vivo activity:

To be determined

	Solvent	mg/mL	mM
Solubility
	DMSO	1.0	2.42

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 413.12 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Anchoori RK, Khan SR, Sueblinvong T, Felthauser A, Iizuka Y, Gavioli R, Destro F, Isaksson Vogel R, Peng S, Roden RB, Bazzaro M. Stressing the ubiquitin-proteasome system without 20S proteolytic inhibition selectively kills cervical cancer cells. PLoS One. 2011;6(8):e23888. doi: 10.1371/journal.pone.0023888. Epub 2011 Aug 31. PMID: 21909374; PMCID: PMC3166081.

In vitro protocol:

In vivo protocol:

To be determined

1 . Anchoori RK, et al. Stressing the ubiquitin-proteasome system without 20S proteolytic inhibition selectively kills cervical cancer cells.PLoS One. 2011;6(8):e23888.