JNJ0966 is a potent and highly selective allosteric inhibitor of matrix metalloproteinase-9 (MMP-9), distinct from traditional active-site zinc chelators. It binds to a regulatory site on the MMP-9 zymogen, preventing its activation without affecting the catalytic activity of the mature enzyme. In enzymatic assays, JNJ0966 exhibited an IC₅₀ of approximately 440 nM for MMP-9 activation inhibition, while showing minimal to no inhibitory effect on other MMP family members such as MMP-2, MMP-1, or MMP-13 at concentrations up to 50 µM. In cell-based models, JNJ0966 reduced MMP-9–dependent extracellular matrix degradation and attenuated neuroinflammation in animal models, highlighting its potential therapeutic utility in MMP-9–driven pathologies with a favorable selectivity profile.
MedKoo Cat#: 564581
Name: JNJ0966
CAS#: 315705-75-0
Chemical Formula: C16H16N4O2S2
Exact Mass: 360.0715
Molecular Weight: 360.45
Elemental Analysis: C, 53.32; H, 4.47; N, 15.54; O, 8.88; S, 17.79
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 63.3 | 175.72 |
The following data is based on the product molecular weight 360.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
