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MedKoo Cat#: 525863 | Name: Prednisolone
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Prednisolone is a glucocorticoid with anti-inflammatory and immunosuppressive activity.

Chemical Structure

Prednisolone
CAS#50-24-8 (free)

Theoretical Analysis

MedKoo Cat#: 525863

Name: Prednisolone

CAS#: 50-24-8 (free)

Chemical Formula: C21H28O5

Exact Mass: 360.1937

Molecular Weight: 360.45

Elemental Analysis: C, 69.98; H, 7.83; O, 22.19

Price and Availability

Size Price Availability Quantity
1g USD 195.00
2g USD 350.00
5g USD 550.00
10g USD 950.00
25g USD 1,650.00
50g USD 2,950.00
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Related CAS #
52-21-1 (acetate) 72064-79-0 (valerate acetate) 630-67-1 (sodium metazoate) 125-02-0 (Na+ phosphate) 50-24-8 (free) 2920-86-7 (hemisuccinate)
Synonym
Prednisolone Phosphate Sodium; AKOS016010152; AK115681; prednisolone; sodium phosphate Predsol; Pediapred;
IUPAC/Chemical Name
(8S,9S,10R,11S,13S,14S,17R)-11,17-Dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-one
InChi Key
OIGNJSKKLXVSLS-VWUMJDOOSA-N
InChi Code
InChI=1S/C21H28O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h5,7,9,14-16,18,22,24,26H,3-4,6,8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
SMILES Code
O=C1C=C[C@]2(C)[C@@]3([H])[C@@H](O)C[C@]4(C)[C@](C(CO)=O)(O)CC[C@@]4([H])[C@]3([H])CCC2=C1
Appearance
White to off-white crystalline powder.
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Prednisolone is a potent, orally active corticosteroid and a glucocorticoid.
In vitro activity:
This study reports here a study of Prednisolone treatment using as a model a leukemia cell line with subsequent investigation of resistance-related gene expression. This study analyzed effects of increasing doses of Prednisolone on ALL cell survival and growth, and monitored immediate effects on gene expression through gene expression assays. Upon treatment with escalating Prednisolone concentration, this study observed a gradual decline in cell survival. Prednisolone competitively induces MCL1 expression. Reference: Adv Exp Med Biol. 2021;1339:147-160. https://pubmed.ncbi.nlm.nih.gov/35023102/
In vivo activity:
This study evaluated the effect of in vivo administration of prednisolone on Escherichia coli lipopolysaccharide endotoxin (LPS)-induced increases in plasma RNI and neutrophil mRNA for NOS II and production of RNI in the rat. This study shows that LPS rapidly induces mRNA for NOS II and production of RNI (NO2- and NO3- anion) in rat neutrophils within 2 hr after in vivo administration of a sublethal dose of 0.5 mg/kg, i.v. A pharmacologic dose of prednisolone (50 micrograms/kg, im) given 15 min before LPS-attenuated production of NO2- and NO3- by neutrophils and suppressed LPS-stimulated mRNA for NOS II. 3-Amino, 1,2,4-triazine inhibited NO2- and NO3- production without affecting gene expression for NOS II. Reference: Proc Soc Exp Biol Med. 1994 Mar;205(3):220-9. https://pubmed.ncbi.nlm.nih.gov/7513433/
Solvent mg/mL mM comments
Solubility
DMF 0.0 83.23
DMSO 54.8 152.12
Ethanol 35.0 97.10
Ethanol:PBS (pH 7.2) (1:1) 0.2 0.55
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 360.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. G L, Adamaki M, Hatziagapiou K, Geronikolou SA, Tsartsalis AN, Vlahopoulos S. Early and Very Early GRIM19 and MCL1 Expression Are Correlated to Late Acquired Prednisolone Effects in a T-Cell Acute Leukemia Cell Line. Adv Exp Med Biol. 2021;1339:147-160. doi: 10.1007/978-3-030-78787-5_20. PMID: 35023102. 2. Li C, Yang P, Liu B, Bu J, Liu H, Guo J, Hasegawa T, Si H, Li M. Prednisolone induces osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway. J Mol Histol. 2021 Oct;52(5):1081-1095. doi: 10.1007/s10735-021-10006-0. Epub 2021 Jul 23. PMID: 34297260. 3. Rioja I, Bush KA, Buckton JB, Dickson MC, Life PF. Joint cytokine quantification in two rodent arthritis models: kinetics of expression, correlation of mRNA and protein levels and response to prednisolone treatment. Clin Exp Immunol. 2004 Jul;137(1):65-73. doi: 10.1111/j.1365-2249.2004.02499.x. PMID: 15196245; PMCID: PMC1809073. 4. Kolls J, Xie J, LeBlanc R, Malinski T, Nelson S, Summer W, Greenberg SS. Rapid induction of messenger RNA for nitric oxide synthase II in rat neutrophils in vivo by endotoxin and its suppression by prednisolone. Proc Soc Exp Biol Med. 1994 Mar;205(3):220-9. doi: 10.3181/00379727-205-43700. PMID: 7513433.
In vitro protocol:
1. G L, Adamaki M, Hatziagapiou K, Geronikolou SA, Tsartsalis AN, Vlahopoulos S. Early and Very Early GRIM19 and MCL1 Expression Are Correlated to Late Acquired Prednisolone Effects in a T-Cell Acute Leukemia Cell Line. Adv Exp Med Biol. 2021;1339:147-160. doi: 10.1007/978-3-030-78787-5_20. PMID: 35023102. 2. Li C, Yang P, Liu B, Bu J, Liu H, Guo J, Hasegawa T, Si H, Li M. Prednisolone induces osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway. J Mol Histol. 2021 Oct;52(5):1081-1095. doi: 10.1007/s10735-021-10006-0. Epub 2021 Jul 23. PMID: 34297260.
In vivo protocol:
1. Rioja I, Bush KA, Buckton JB, Dickson MC, Life PF. Joint cytokine quantification in two rodent arthritis models: kinetics of expression, correlation of mRNA and protein levels and response to prednisolone treatment. Clin Exp Immunol. 2004 Jul;137(1):65-73. doi: 10.1111/j.1365-2249.2004.02499.x. PMID: 15196245; PMCID: PMC1809073. 2. Kolls J, Xie J, LeBlanc R, Malinski T, Nelson S, Summer W, Greenberg SS. Rapid induction of messenger RNA for nitric oxide synthase II in rat neutrophils in vivo by endotoxin and its suppression by prednisolone. Proc Soc Exp Biol Med. 1994 Mar;205(3):220-9. doi: 10.3181/00379727-205-43700. PMID: 7513433.
1: Hayashi S, Akao N, Okamoto K. Meningeal plasma cell granuloma in the early stage of relapsing polychondritis. Rinsho Shinkeigaku. 2017 May 26. doi: 10.5692/clinicalneurol.cn-000975. [Epub ahead of print] PubMed PMID: 28552866. 2: Nived P, Nagel J, Saxne T, Geborek P, Jönsson G, Skattum L, Kapetanovic MC. Immune response to pneumococcal conjugate vaccine in patients with systemic vasculitis receiving standard of care therapy. Vaccine. 2017 May 25. pii: S0264-410X(17)30680-1. doi: 10.1016/j.vaccine.2017.05.044. [Epub ahead of print] PubMed PMID: 28552512. 3: Padberg S, Mick I, Frenzel C, Greil R, Hilberath J, Schaefer C. Transient congenital dilated cardiomyopathy after maternal R-CHOP chemotherapy during pregnancy. Reprod Toxicol. 2017 May 24. pii: S0890-6238(16)30498-1. doi: 10.1016/j.reprotox.2017.05.008. [Epub ahead of print] PubMed PMID: 28552383. 4: van der Aa NE, Grotenhuis HB, Breur HJM. Remarkable improvement in mitral valve regurgitation in paediatric eosinophilic granuloma with polyangiitis. Cardiol Young. 2017 May 29:1-3. doi: 10.1017/S1047951117000889. [Epub ahead of print] PubMed PMID: 28552076. 5: Kawazoe M, Kaneko K, Shikano K, Kusunoki N, Nanki T, Kawai S. Glucocorticoid therapy causes contradictory changes of serum Wnt signaling-related molecules in systemic autoimmune diseases. Clin Rheumatol. 2017 May 27. doi: 10.1007/s10067-017-3689-3. [Epub ahead of print] PubMed PMID: 28551822. 6: Sylvester B, Porfire A, Van Bockstal PJ, Porav S, Achim M, Beer T, Tomuță I. Formulation Optimization of Freeze-Dried Long-Circulating Liposomes and In-Line Monitoring of the Freeze-Drying Process using an NIR Spectroscopy Tool. J Pharm Sci. 2017 May 24. pii: S0022-3549(17)30381-7. doi: 10.1016/j.xphs.2017.05.024. [Epub ahead of print] PubMed PMID: 28551424. 7: Serra SS, Pedrosa T, Falcão S, Branco JC. [Severe Interstitial Lung Disease and Manic Symptoms Secondary to Corticosteroids in a Patient with Systemic Lupus Erythematosus and Secondary Sjögren's Syndrome]. Acta Med Port. 2017 Mar 31;30(3):246-250. doi: 10.20344/amp.7297. Epub 2017 Mar 31. Portuguese. PubMed PMID: 28550835. 8: Mu X, Tang Y, Takayama K, Chen W, Lu A, Wang B, Weiss K, Huard J. RhoA/Rock Inhibition Improves the Beneficial Effects of Glucocorticoid Treatment in Dystrophic Muscle: Implications for Stem Cell Depletion. Hum Mol Genet. 2017 May 26. doi: 10.1093/hmg/ddx117. [Epub ahead of print] PubMed PMID: 28549178. 9: Karras A, Pagnoux C, Haubitz M, Groot K, Puechal X, Tervaert JWC, Segelmark M, Guillevin L, Jayne D; European Vasculitis Society.. Randomised controlled trial of prolonged treatment in the remission phase of ANCA-associated vasculitis. Ann Rheum Dis. 2017 May 25. pii: annrheumdis-2017-211123. doi: 10.1136/annrheumdis-2017-211123. [Epub ahead of print] PubMed PMID: 28546260. 10: Weckmann M, Becker T, Nissen G, Pech M, Kopp MV. SiMA: A simplified migration assay for analyzing neutrophil migration. Cytometry A. 2017 May 19. doi: 10.1002/cyto.a.23114. [Epub ahead of print] PubMed PMID: 28544679. 11: Workman AD, Carey RM, Kohanski MA, Adappa ND, Palmer JN, Cohen NA. Effects of ophthalmologic solutions on sinonasal ciliated epithelium. Int Forum Allergy Rhinol. 2017 May 23. doi: 10.1002/alr.21953. [Epub ahead of print] PubMed PMID: 28544585. 12: Georgakopoulou EA, Achtari MD. Oral lichenoid lesions of the upper lip. J Dermatol Case Rep. 2017 Mar 31;11(1):16-19. doi: 10.3315/jdcr.2017.1243. eCollection 2017 Mar 31. PubMed PMID: 28539985; PubMed Central PMCID: PMC5439692. 13: Saito A, Takeuchi Y, Kagaya S, Ojima Y, Fukami H, Sato H, Matsuda K, Nagasawa T. Remission Induction Therapy with Rituximab for Microscopic Polyangiitis: A Feasibility Study. Tohoku J Exp Med. 2017;242(1):53-62. doi: 10.1620/tjem.242.53. PubMed PMID: 28539536. 14: Kono M, Hasegawa T, Nagai S, Odani T, Akikawa K, Nomura Y, Sato H, Kikuchi K, Amizuka N, Kikuchi H. Palisaded neutrophilic and granulomatous dermatitis as a novel cause of hypercalcemia: A case report. Medicine (Baltimore). 2017 May;96(21):e6968. doi: 10.1097/MD.0000000000006968. PubMed PMID: 28538395. 15: Nagai N, Ibuki M, Shinoda H, Kameyama K, Tsubota K, Ozawa Y. Maculopapular rash after intravitreal injection of an antivascular endothelial growth factor, aflibercept, for treating age-related macular degeneration: A case report. Medicine (Baltimore). 2017 May;96(21):e6965. doi: 10.1097/MD.0000000000006965. PubMed PMID: 28538392. 16: Coler-Reilly ALG, Sato T, Matsuzaki T, Nakagawa M, Niino M, Nagai M, Nakamura T, Takenouchi N, Araya N, Yagishita N, Inoue E, Yamano Y. Effectiveness of Daily Prednisolone to Slow Progression of Human T-Lymphotropic Virus Type 1-Associated Myelopathy/Tropical Spastic Paraparesis: A Multicenter Retrospective Cohort Study. Neurotherapeutics. 2017 May 23. doi: 10.1007/s13311-017-0533-z. [Epub ahead of print] PubMed PMID: 28536850. 17: Chun BY, Kim SY. Acute visual loss after botulinum toxin A injection in the masseter muscle. Int Ophthalmol. 2017 May 23. doi: 10.1007/s10792-017-0569-4. [Epub ahead of print] PubMed PMID: 28536763. 18: Finlay JR, Wyatt K, North C. Recovery from Cyclophosphamide Overdose in a Dog. J Am Anim Hosp Assoc. 2017 May 23. doi: 10.5326/JAAHA-MS-6411. [Epub ahead of print] PubMed PMID: 28535135. 19: Lau ETL, Johnson SK, Williams BA, Mikkelsen D, McCourt E, Stanley RA, Mereddy R, Halley PJ, Steadman KJ. Optimizing Prednisolone Loading into Distiller's Dried Grain Kafirin Microparticles, and In vitro Release for Oral Delivery. Pharmaceutics. 2017 May 19;9(2). pii: E17. doi: 10.3390/pharmaceutics9020017. PubMed PMID: 28534827. 20: Lin WT, Chen YM, Chen DY, Lan JL, Chang CS, Yeh HZ, Yang SS. Increased risk of hepatitis B virus reactivation in systemic lupus erythematosus patients receiving immunosuppressants: a retrospective cohort study. Lupus. 2017 Jan 1:961203317711009. doi: 10.1177/0961203317711009. [Epub ahead of print] PubMed PMID: 28534427.