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MedKoo Cat#: 531307 | Name: STF-62247
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

STF-62247 is a potent autophagy inducer. STF-62247 shows selective toxicity and growth inhibition of renal cells lacking VHL; 25-fold greater sensitivity observed for cells with VHL deficiency compared to wild-type (VHL+).

Chemical Structure

STF-62247
STF-62247
CAS#315702-99-9

Theoretical Analysis

MedKoo Cat#: 531307

Name: STF-62247

CAS#: 315702-99-9

Chemical Formula: C15H13N3S

Exact Mass: 267.0830

Molecular Weight: 267.35

Elemental Analysis: C, 67.39; H, 4.90; N, 15.72; S, 11.99

Price and Availability

Size Price Availability Quantity
50mg USD 450.00 2 Weeks
100mg USD 750.00 2 Weeks
200mg USD 1,250.00 2 Weeks
500mg USD 2,750.00 2 Weeks
1g USD 3,850.00 2 Weeks
2g USD 6,250.00 2 Weeks
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Related CAS #
No Data
Synonym
STF-62247; STF 62247; STF62247; STF-062247; STF 062247; STF062247;
IUPAC/Chemical Name
4-(pyridin-4-yl)-N-m-tolylthiazol-2-amine
InChi Key
KATNUHQNJGNLPW-UHFFFAOYSA-N
InChi Code
InChI=1S/C15H13N3S/c1-11-3-2-4-13(9-11)17-15-18-14(10-19-15)12-5-7-16-8-6-12/h2-10H,1H3,(H,17,18)
SMILES Code
CC1=CC(NC2=NC(C3=CC=NC=C3)=CS2)=CC=C1
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
STF-62247 is selectively cytotoxic to VHL-deficient renal cell carcinoma (IC50 of 0.625 μM and 16 μM in RCC4 and RCC4/VHL cells, respectively).
In vitro activity:
STF-62247 modulated cellular metabolism of glutamine to support lipogenesis, which could be implicated in the signaling driving to cell death. STF-62247 stimulated expression of stearoyl-CoA desaturase (SCD1) and peripilin2 (PLIN2) to generate accumulation of lipid droplets in VHL-deficient cells. Carnitine palmitoyltransferase 1A (CPT1A) also increased in response to STF-62247. CPT1A overexpression in Clear Cell Renal Cell Carcinoma (ccRCC) is known to limit tumor growth. Reference: Front Oncol. 2022 Feb 9;12:841054. https://pubmed.ncbi.nlm.nih.gov/35223522/
In vivo activity:
To be determined
Solvent mg/mL mM
Solubility
DMF 25.0 93.51
DMSO 25.0 93.51
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 267.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Johnson M, Nowlan S, Sahin G, Barnett DA, Joy AP, Touaibia M, Cuperlovic-Culf M, Zofija Avizonis D, Turcotte S. Decrease of Intracellular Glutamine by STF-62247 Results in the Accumulation of Lipid Droplets in von Hippel-Lindau Deficient Cells. Front Oncol. 2022 Feb 9;12:841054. doi: 10.3389/fonc.2022.841054. PMID: 35223522; PMCID: PMC8865074. 2. Turcotte S, Chan DA, Sutphin PD, Hay MP, Denny WA, Giaccia AJ. A molecule targeting VHL-deficient renal cell carcinoma that induces autophagy. Cancer Cell. 2008 Jul 8;14(1):90-102. doi: 10.1016/j.ccr.2008.06.004. PMID: 18598947; PMCID: PMC2819422.
In vitro protocol:
1. Johnson M, Nowlan S, Sahin G, Barnett DA, Joy AP, Touaibia M, Cuperlovic-Culf M, Zofija Avizonis D, Turcotte S. Decrease of Intracellular Glutamine by STF-62247 Results in the Accumulation of Lipid Droplets in von Hippel-Lindau Deficient Cells. Front Oncol. 2022 Feb 9;12:841054. doi: 10.3389/fonc.2022.841054. PMID: 35223522; PMCID: PMC8865074. 2. Turcotte S, Chan DA, Sutphin PD, Hay MP, Denny WA, Giaccia AJ. A molecule targeting VHL-deficient renal cell carcinoma that induces autophagy. Cancer Cell. 2008 Jul 8;14(1):90-102. doi: 10.1016/j.ccr.2008.06.004. PMID: 18598947; PMCID: PMC2819422.
In vivo protocol:
To be determined
1: Ma T, Li D, Yang ZR, Wang Q, Chen R, Lv N, Du K, Qin H, Tao J, Jiang H, Zhu J. Autophagy activator-loaded bicomponent peptide nanocarriers for phototherapy- triggered immunity enhancement against metastatic breast cancer. J Control Release. 2024 Oct 14;376:241-252. doi: 10.1016/j.jconrel.2024.10.009. Epub ahead of print. PMID: 39384152. 2: Jiang J, Du Z, Tang H, Huang Y, Li D, Liang Q. Comprehensive Analysis and Experimental Validation of TLL2 as a Potential New Prognostic Biomarker Associated with Immune Infiltration in Lung Adenocarcinoma. Recent Pat Anticancer Drug Discov. 2024 Aug 26. doi: 10.2174/0115748928303392240817131807. Epub ahead of print. PMID: 39192647. 3: Meng Y, Lyu CC, He YT, Che HY, Jiang H, Zhang JB, Tang HY, Yuan B. ALG5 Regulates STF-62247-Induced Milk Fat Synthesis via the mTOR Signaling Pathway. J Agric Food Chem. 2024 Jul 3;72(26):14620-14629. doi: 10.1021/acs.jafc.3c07812. Epub 2024 Jun 17. PMID: 38885170. 4: Li M, Zhao D, Yan J, Fu X, Li F, Liu G, Fan Y, Liang Y, Zhang X. A Redox- Triggered Autophagy-Induced Nanoplatform with PD-L1 Inhibition for Enhancing Combined Chemo-Immunotherapy. ACS Nano. 2024 May 21;18(20):12870-12884. doi: 10.1021/acsnano.4c00227. Epub 2024 May 10. PMID: 38727063. 5: Ding X, Wan A, Qi X, Jiang K, Liu Z, Chen B. ZNF695, A Potential Prognostic Biomarker, Correlates with Im mune Infiltrates in Cervical Squamous Cell Carcinoma and Endoce rvical Adenocarcinoma: Bioinformatic Analysis and Experimental Verification. Curr Gene Ther. 2024;24(5):441-452. doi: 10.2174/0115665232285216240228071244. PMID: 38441026. 6: Zhang S, Huang Y, Pi S, Chen H, Ye F, Wu C, Li L, Ye Q, Lin Y, Su Z. Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA. J Nanobiotechnology. 2023 Oct 3;21(1):360. doi: 10.1186/s12951-023-02067-y. PMID: 37789342; PMCID: PMC10548684. 7: Long X, Wang H, Yan J, Li Y, Dong X, Tian S, Sun Y, Luo K, He B, Liang Y. Tailor-Made Autophagy Cascade Amplification Polymeric Nanoparticles for Enhanced Tumor Immunotherapy. Small. 2023 Jun;19(24):e2207898. doi: 10.1002/smll.202207898. Epub 2023 Mar 18. PMID: 36932938. 8: Aparicio IM, Rojo-Domínguez P, Castillejo-Rufo A, Peña FJ, Tapia JA. The Autophagy Marker LC3 Is Processed during the Sperm Capacitation and the Acrosome Reaction and Translocates to the Acrosome Where It Colocalizes with the Acrosomal Membranes in Horse Spermatozoa. Int J Mol Sci. 2023 Jan 4;24(2):937. doi: 10.3390/ijms24020937. PMID: 36674454; PMCID: PMC9862423. 9: Johnson M, Nowlan S, Sahin G, Barnett DA, Joy AP, Touaibia M, Cuperlovic-Culf M, Zofija Avizonis D, Turcotte S. Decrease of Intracellular Glutamine by STF-62247 Results in the Accumulation of Lipid Droplets in von Hippel-Lindau Deficient Cells. Front Oncol. 2022 Feb 9;12:841054. doi: 10.3389/fonc.2022.841054. PMID: 35223522; PMCID: PMC8865074. 10: Wang X, Li M, Ren K, Xia C, Li J, Yu Q, Qiu Y, Lu Z, Long Y, Zhang Z, He Q. On-Demand Autophagy Cascade Amplification Nanoparticles Precisely Enhanced Oxaliplatin-Induced Cancer Immunotherapy. Adv Mater. 2020 Aug;32(32):e2002160. doi: 10.1002/adma.202002160. Epub 2020 Jun 28. PMID: 32596861. 11: Kinzler MN, Zielke S, Kardo S, Meyer N, Kögel D, van Wijk SJL, Fulda S. STF-62247 and pimozide induce autophagy and autophagic cell death in mouse embryonic fibroblasts. Sci Rep. 2020 Jan 20;10(1):687. doi: 10.1038/s41598-019-56990-y. PMID: 31959760; PMCID: PMC6971264. 12: Bouhamdani N, Comeau D, Coholan A, Cormier K, Turcotte S. Targeting lysosome function causes selective cytotoxicity in VHL-inactivated renal cell carcinomas. Carcinogenesis. 2020 Jul 10;41(6):828-840. doi: 10.1093/carcin/bgz161. PMID: 31556451; PMCID: PMC7351131. 13: Bouhamdani N, Comeau D, Cormier K, Turcotte S. STF-62247 accumulates in lysosomes and blocks late stages of autophagy to selectively target von Hippel- Lindau-inactivated cells. Am J Physiol Cell Physiol. 2019 May 1;316(5):C605-C620. doi: 10.1152/ajpcell.00483.2018. Epub 2019 Feb 13. PMID: 30758995. 14: Zielke S, Meyer N, Mari M, Abou-El-Ardat K, Reggiori F, van Wijk SJL, Kögel D, Fulda S. Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells. Cell Death Dis. 2018 Sep 24;9(10):994. doi: 10.1038/s41419-018-1003-1. PMID: 30250198; PMCID: PMC6155211. 15: Kozako T, Sato K, Uchida Y, Kato N, Aikawa A, Ogata K, Kamimura H, Uemura H, Yoshimitsu M, Ishitsuka K, Higaki Y, Tanaka H, Honda SI, Soeda S. The small molecule STF-62247 induces apoptotic and autophagic cell death in leukemic cells. Oncotarget. 2018 Jun 12;9(45):27645-27655. doi: 10.18632/oncotarget.25291. PMID: 29963226; PMCID: PMC6021257. 16: Bouhamdani N, Joy A, Barnett D, Cormier K, Léger D, Chute IC, Lamarre S, Ouellette R, Turcotte S. Quantitative proteomics to study a small molecule targeting the loss of von Hippel-Lindau in renal cell carcinomas. Int J Cancer. 2017 Aug 15;141(4):778-790. doi: 10.1002/ijc.30774. Epub 2017 May 25. PMID: 28486780. 17: Aparicio IM, Martin Muñoz P, Salido GM, Peña FJ, Tapia JA. The autophagy- related protein LC3 is processed in stallion spermatozoa during short-and long- term storage and the related stressful conditions. Animal. 2016 Jul;10(7):1182-91. doi: 10.1017/S1751731116000240. Epub 2016 Mar 2. PMID: 26932581. 18: Anbalagan S, Pires IM, Blick C, Hill MA, Ferguson DJ, Chan DA, Hammond EM. Radiosensitization of renal cell carcinoma in vitro through the induction of autophagy. Radiother Oncol. 2012 Jun;103(3):388-93. doi: 10.1016/j.radonc.2012.04.001. Epub 2012 Apr 30. PMID: 22551566. 19: Zimmerman BE, Palm S. Results of an international comparison of 57Co. Appl Radiat Isot. 2010 Jul-Aug;68(7-8):1217-20. doi: 10.1016/j.apradiso.2009.12.021. Epub 2009 Dec 16. PMID: 20071188. 20: Chan DA, Giaccia AJ. Targeting cancer cells by synthetic lethality: autophagy and VHL in cancer therapeutics. Cell Cycle. 2008 Oct;7(19):2987-90. doi: 10.4161/cc.7.19.6776. Epub 2008 Oct 12. PMID: 18818511.