Synonym
KY02111; KY 02111; KY-02111
IUPAC/Chemical Name
N-(6-Chloro-2-benzothiazolyl)-3,4-dimethoxybenzenepropanamide
InChi Key
LXFKEVQQSKQXPR-UHFFFAOYSA-N
InChi Code
InChI=1S/C18H17ClN2O3S/c1-23-14-7-3-11(9-15(14)24-2)4-8-17(22)21-18-20-13-6-5-12(19)10-16(13)25-18/h3,5-7,9-10H,4,8H2,1-2H3,(H,20,21,22)
SMILES Code
O=C(NC1=NC2=CC=C(Cl)C=C2S1)CCC3=CC=C(OC)C(OC)=C3
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO, not in water
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
KY02111 is a canonical WNT signaling (β-catenin) inhibitor which promotes differentiation of hPSCs to cardiomyocytes. KY02111 can be used for the research of human cardiomyocyte regeneration.
In vitro activity:
To gain insight into how KY02111 promotes cardiac differentiation of hPSCs, gene expression profiles of IMR90-1 hiPSCs treated with KY02111 were analyzed using the microarray technique. KY02111 or DMSO was added to the culture on day 3 of cardiac differentiation, and the cell population was harvested after 12 or 24 hr. A total of 22 downregulated genes and four upregulated genes were identified at both 12 and 24 hr after KY02111 treatment (Figure 3A; Table S1). Although only a few genes were extracted by the microarray analysis, perhaps due to high heterogeneity of the cell population, the microarray data were validated by qPCR (Figure S4A). When the 22 downregulated genes were examined for common transcription factor-binding sites, using DiRE (Gotea and Ovcharenko, 2008), which can predict distant regulatory elements, TCF4 was predicted as a common transcription factor (Figure S4B). Of the 22 downregulated genes, 16 (72.7%) were known target genes of canonical WNT signaling (Table S1). Furthermore, the effect of KY02111 on WNT target gene expression was very similar to that of other WNT inhibitors, such as XAV939 and IWP-2, but not of BIO, a WNT activator (Figure S4C). These results suggested that KY02111 inhibits canonical WNT signaling in hPSCs.
Reference: Cell Rep. 2012 Nov 29;2(5):1448-60. https://pubmed.ncbi.nlm.nih.gov/23103164/
|
Solvent |
mg/mL |
mM |
| Solubility |
| DMSO |
34.3 |
91.19 |
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
376.86
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
1. Morgan JT, Raghunathan VK, Chang YR, Murphy CJ, Russell P. Wnt inhibition induces persistent increases in intrinsic stiffness of human trabecular meshwork cells. Exp Eye Res. 2015 Mar;132:174-8. doi: 10.1016/j.exer.2015.01.025. Epub 2015 Jan 30. PMID: 25639201; PMCID: PMC4352377. 2. Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N. A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep. 2012 Nov 29;2(5):1448-60. doi: 10.1016/j.celrep.2012.09.015. Epub 2012 Oct 25. PMID: 23103164.
In vitro protocol:
1. Morgan JT, Raghunathan VK, Chang YR, Murphy CJ, Russell P. Wnt inhibition induces persistent increases in intrinsic stiffness of human trabecular meshwork cells. Exp Eye Res. 2015 Mar;132:174-8. doi: 10.1016/j.exer.2015.01.025. Epub 2015 Jan 30. PMID: 25639201; PMCID: PMC4352377. 2. Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N. A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep. 2012 Nov 29;2(5):1448-60. doi: 10.1016/j.celrep.2012.09.015. Epub 2012 Oct 25. PMID: 23103164.
1: Morgan JT, Raghunathan VK, Chang YR, Murphy CJ, Russell P. Wnt inhibition induces persistent increases in intrinsic stiffness of human trabecular meshwork cells. Exp Eye Res. 2015 Mar;132:174-8. doi: 10.1016/j.exer.2015.01.025. Epub 2015 Jan 30. PubMed PMID: 25639201; PubMed Central PMCID: PMC4352377.
2: Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N. A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep. 2012 Nov 29;2(5):1448-60. doi: 10.1016/j.celrep.2012.09.015. Epub 2012 Oct 25. PubMed PMID: 23103164.
