NU6140 | CAS#444723-13-1 | CDK inhibitor

MedKoo Cat#: 406352 | Name: NU6140

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

NU6140 is a cyclin-dependent kinase 2 (cdk2) inhibitor; induces cell-cycle arrest at the G2-M phase.

Chemical Structure

NU6140

CAS#444723-13-1

Theoretical Analysis

MedKoo Cat#: 406352

Name: NU6140

CAS#: 444723-13-1

Chemical Formula: C23H30N6O2

Exact Mass: 422.2430

Molecular Weight: 422.53

Elemental Analysis: C, 65.38; H, 7.16; N, 19.89; O, 7.57

Price and Availability

Size	Price	Availability	Quantity
5mg	USD 335.00	2 Weeks
10mg	USD 610.00	2 Weeks

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Related CAS #

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Synonym

NU6140; NU-6140; NU 6140.

IUPAC/Chemical Name

4-((6-(cyclohexylmethoxy)-1H-purin-2-yl)amino)-N,N-diethylbenzamide

InChi Key

XHEQSRJCJTWWAH-UHFFFAOYSA-N

InChi Code

InChI=1S/C23H30N6O2/c1-3-29(4-2)22(30)17-10-12-18(13-11-17)26-23-27-20-19(24-15-25-20)21(28-23)31-14-16-8-6-5-7-9-16/h10-13,15-16H,3-9,14H2,1-2H3,(H2,24,25,26,27,28)

SMILES Code

O=C(N(CC)CC)C1=CC=C(NC2=NC3=NC=NC3=C(OCC4CCCCC4)N2)C=C1

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

soluble in DMSO, not soluble in water

Shelf Life

>2 years if stored properly.

Drug Formulation

This drug may be formulated in DMSO.

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

NU6140 is a selective CDK2-cyclin A inhibitor (IC50, 0.41 μM).

In vitro activity:

This study investigated the effects of the small molecular agent NU6140 (inhibits CDK2 and cyclin A interaction) on human embryonic stem (hES) cells and embryonal carcinoma-derived (hEC) cells via the expression of transcription factors responsible for pluripotency. Both hES and hEC cells responded to NU6140 treatment by induced apoptosis and a decreased expression of NANOG, OCT4, and SOX2 in surviving cells. A higher sensitivity to NU6140 application in hES than hEC cells was detected. NU6140 treatment arrested hES and hEC cells in the G2 phase and inhibited entry into the M phase as evidenced by no significant increase in histone 3 phosphorylation. Reference: Int J Cell Biol. 2014;2014:280638. https://pubmed.ncbi.nlm.nih.gov/25477962/

In vivo activity:

TBD

	Solvent	mg/mL	mM
Solubility
	DMF	30.0	71.00
	DMSO	107.4	254.22
	Ethanol	36.1	85.50
	Ethanol:PBS (pH 7.2) (1:3)	0.3	0.59

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 422.53 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Kallas A, Pook M, Trei A, Maimets T. Assessment of the Potential of CDK2 Inhibitor NU6140 to Influence the Expression of Pluripotency Markers NANOG, OCT4, and SOX2 in 2102Ep and H9 Cells. Int J Cell Biol. 2014;2014:280638. doi: 10.1155/2014/280638. Epub 2014 Nov 17. PMID: 25477962; PMCID: PMC4248398. 2. Bhattacharya S, Ray RM, Johnson LR. Cyclin-dependent kinases regulate apoptosis of intestinal epithelial cells. Apoptosis. 2014 Mar;19(3):451-66. doi: 10.1007/s10495-013-0942-3. PMID: 24242917; PMCID: PMC3945523.

In vitro protocol:

In vivo protocol:

TBD

1: Pennati M, Campbell AJ, Curto M, Binda M, Cheng Y, Wang LZ, Curtin N, Golding BT, Griffin RJ, Hardcastle IR, Henderson A, Zaffaroni N, Newell DR. Potentiation of paclitaxel-induced apoptosis by the novel cyclin-dependent kinase inhibitor NU6140: a possible role for survivin down-regulation. Mol Cancer Ther. 2005 Sep;4(9):1328-37. PubMed PMID: 16170024.