HUP-46 is a PREP inhibitor. HUP-46, a 5-aminothiazole derivative reported in the 2024 Journal of Medicinal Chemistry article (DOI: 10.1021/acs.jmedchem.3c01993), is a weak prolyl oligopeptidase (PREP) inhibitor (IC₅₀ = 8 μM) but a potent modulator of its protein–protein interaction (PPI)-derived functions. It significantly reduces α-synuclein dimerization (74% of control) and enhances autophagic flux (77% of control) at 10 μM. HUP-46 also activates PP2A with an EC₅₀ of ~100 nM and is brain-penetrant. Its effects are PREP-specific and dependent on a novel binding site distinct from the proteolytic active site.
MedKoo Cat#: 126650
Name: HUP-46
CAS#: 3007689-83-7
Chemical Formula: C18H21N3S
Exact Mass: 311.1456
Molecular Weight: 311.45
Elemental Analysis: C, 69.42; H, 6.80; N, 13.49; S, 10.29
The following data is based on the product molecular weight 311.45 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
