MedKoo Cat#: 125684 | Name: Rhod-5N potassium
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Rhod-5N is a low affinity fluorescent calcium probe (Kd = 320 µM).

Chemical Structure

Rhod-5N potassium
Rhod-5N potassium
CAS#888229-31-0

Theoretical Analysis

MedKoo Cat#: 125684

Name: Rhod-5N potassium

CAS#: 888229-31-0

Chemical Formula: C39H36K3N5O13

Exact Mass: 899.1200

Molecular Weight: 900.03

Elemental Analysis: C, 52.05; H, 4.03; K, 13.03; N, 7.78; O, 23.11

Price and Availability

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1mg USD 650.00 2 Weeks
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Related CAS #
No Data
Synonym
Rhod-5N (potassium salt); Rhod-5N potassium;
IUPAC/Chemical Name
potassium 2,2'-((2-(2-(2-(bis(carboxylatomethyl)amino)-5-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)phenoxy)ethoxy)-4-nitrophenyl)azanediyl)diacetate
InChi Key
JLZGTFWLMRDWKD-UHFFFAOYSA-K
InChi Code
InChI=1S/C39H39N5O13.3K/c1-40(2)24-6-9-27-31(16-24)57-32-17-25(41(3)4)7-10-28(32)39(27)23-5-11-29(42(19-35(45)46)20-36(47)48)33(15-23)55-13-14-56-34-18-26(44(53)54)8-12-30(34)43(21-37(49)50)22-38(51)52;;;/h5-12,15-18H,13-14,19-22H2,1-4H3,(H3-,45,46,47,48,49,50,51,52);;;/q;3*+1/p-3
SMILES Code
CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=CC(=C(C=C4)N(CC(=O)[O-])CC(=O)[O-])OCCOC5=C(C=CC(=C5)[N+](=O)[O-])N(CC(=O)[O-])CC(=O)[O-].[K+].[K+].[K+]
Appearance
To be determined
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 -4 C for short term (days to weeks) or -20 C for long term(months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info

Preparing Stock Solutions

The following data is based on the product molecular weight 900.03 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Soibinet M, Souchon V, Leray I, Valeur B. Rhod-5N as a fluorescent molecular sensor of cadmium(II) ion. J Fluoresc. 2008 Nov;18(6):1077-82. doi: 10.1007/s10895-008-0352-z. Epub 2008 May 17. PMID: 18488146. 2: de la Fuente S, Fonteriz RI, Montero M, Alvarez J. Dynamics of mitochondrial [Ca(2+)] measured with the low-Ca(2+)-affinity dye rhod-5N. Cell Calcium. 2012 Jan;51(1):65-71. doi: 10.1016/j.ceca.2011.10.007. Epub 2011 Nov 30. PMID: 22133611. 3: McNamara CJ, Perry TD 4th, Bearce K, Hernandez-Duque G, Mitchell R. Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N. J Microbiol Methods. 2005 May;61(2):245-50. doi: 10.1016/j.mimet.2004.12.004. Epub 2004 Dec 30. PMID: 15722151. 4: Ribou AC, Salmon JM, Vigo J, Goyet C. Measurements of calcium with a fluorescent probe Rhod-5N: Influence of high ionic strength and pH. Talanta. 2007 Jan 15;71(1):437-42. doi: 10.1016/j.talanta.2006.04.011. Epub 2006 May 24. PMID: 19071324.