2A3 is an electrophilic chemical probe that acylates the 2'-OH in the RNA backbone. 2A3 can be used for RNA SHAPE-MaP experiments in cell lines or live bacteria. It labels single-stranded or flexible regions more than folded regions (SHAPE methodology) and enables analysis of RNA secondary structures at single nucleotide resolution. For intracellular experiments, 2A3 has higher signal-to-noise ratio and prediction accuracy than NAI. 2A3 is cell permeable, and can probe both gram-positive and gram-negative bacteria.
MedKoo Cat#: 466241
Name: 2A3
CAS#: 2765091-45-8
Chemical Formula: C9H8N4O
Exact Mass: 188.0698
Molecular Weight: 188.19
Elemental Analysis: C, 57.44; H, 4.29; N, 29.77; O, 8.50
| Solvent | mg/mL | mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 18.8 | 100.00 |
The following data is based on the product molecular weight 188.19 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
