CKK-E12 | CAS# 1432494-65-9 | Ionizable lipid

MedKoo Cat#: 465718 | Name: CKK-E12

Description:

WARNING: This product is for research use only, not for human or veterinary use.

CKK-E12 is an ionizable lipid. It is useful for development of lipidoid nanoparticles for siRNA, mRNA, and vaccine delivery.

Chemical Structure

CKK-E12

CAS#1432494-65-9

Theoretical Analysis

MedKoo Cat#: 465718

Name: CKK-E12

CAS#: 1432494-65-9

Chemical Formula: C60H120N4O6

Exact Mass: 992.9208

Molecular Weight: 993.64

Elemental Analysis: C, 72.53; H, 12.17; N, 5.64; O, 9.66

Price and Availability

Size	Price	Availability
10mg	USD 150.00	Ready to Ship
25mg	USD 250.00	Ready to Ship
50mg	USD 450.00	Ready to Ship
100mg	USD 750.00	Ready to Ship
200mg	USD 1,250.00	Ready to Ship
500mg	USD 2,650.00	Ready to Ship
1g	USD 3,850.00	Ready to Ship

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Related CAS #

1432494-65-9

Synonym

CKK-E12; CKKE12; CKK E12; O-8744; O8744; O 8744;

IUPAC/Chemical Name

3,6-bis(4-(bis(2-hydroxydodecyl)amino)butyl)piperazine-2,5-dione

InChi Key

ZISVTYVLWSZJAL-UHFFFAOYSA-N

InChi Code

InChI=1S/C60H120N4O6/c1-5-9-13-17-21-25-29-33-41-53(65)49-63(50-54(66)42-34-30-26-22-18-14-10-6-2)47-39-37-45-57-59(69)62-58(60(70)61-57)46-38-40-48-64(51-55(67)43-35-31-27-23-19-15-11-7-3)52-56(68)44-36-32-28-24-20-16-12-8-4/h53-58,65-68H,5-52H2,1-4H3,(H,61,70)(H,62,69)

SMILES Code

O=C(C(CCCCN(CC(O)CCCCCCCCCC)CC(CCCCCCCCCC)O)N1)NC(CCCCN(CC(CCCCCCCCCC)O)CC(CCCCCCCCCC)O)C1=O

Appearance

Pale yellow viscous liquid

Purity

>95% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Certificate of Analysis

View CoA: current batch, Lot#Y23X02M05

QC Data

View QC data: current batch, Lot#Y23X02M05

Safety Data Sheet (SDS)

View Safety Data Sheet (SDS)

Instruction

View handling instruction

Biological target:

CKK-E12 is a ionizable lipid in combination with other lipids make up the lipid nanoparticles which are used to deliver RNA-based therapeutics.

In vitro activity:

To evaluate the silencing activity of cKK-E12 LPNs in different organs, this study formulated the nanoparticles with siRNA against Phosphatase and tensin homolog (Pten), a ubiquitously expressed protein in different cell types, and measured the expression level of Pten in different tissues after i.v. administration. cKK-E12 LPNs showed significant silencing in the liver over that seen in the lung, spleen, kidney, heart, and brain (Fig. 4A). Reference: Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):3955-60. https://pubmed.ncbi.nlm.nih.gov/24516150/

In vivo activity:

cKK-E12 was highly selective toward liver parenchymal cell in vivo, with orders of magnitude lower doses needed to silence in hepatocytes compared with endothelial cells and immune cells in different organs. Toxicity studies showed that cKK-E12 was well tolerated in rats at a dose of 1 mg/kg (over 100-fold higher than the ED50). Reference: Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):3955-60. https://pubmed.ncbi.nlm.nih.gov/24516150/

	Solvent	mg/mL	mM
Solubility
	DMSO	50.0	50.32

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 993.64 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Dong Y, Love KT, Dorkin JR, Sirirungruang S, Zhang Y, Chen D, Bogorad RL, Yin H, Chen Y, Vegas AJ, Alabi CA, Sahay G, Olejnik KT, Wang W, Schroeder A, Lytton-Jean AK, Siegwart DJ, Akinc A, Barnes C, Barros SA, Carioto M, Fitzgerald K, Hettinger J, Kumar V, Novobrantseva TI, Qin J, Querbes W, Koteliansky V, Langer R, Anderson DG. Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates. Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):3955-60. doi: 10.1073/pnas.1322937111. Epub 2014 Feb 10. Erratum in: Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5753. PMID: 24516150; PMCID: PMC3964096.

In vitro protocol:

In vivo protocol:

1: Melamed JR, Hajj KA, Chaudhary N, Strelkova D, Arral ML, Pardi N, Alameh MG, Miller JB, Farbiak L, Siegwart DJ, Weissman D, Whitehead KA. Lipid nanoparticle chemistry determines how nucleoside base modifications alter mRNA delivery. J Control Release. 2021 Nov 18;341:206-214. doi: 10.1016/j.jconrel.2021.11.022. Epub ahead of print. PMID: 34801660. 2: Dong Y, Love KT, Dorkin JR, Sirirungruang S, Zhang Y, Chen D, Bogorad RL, Yin H, Chen Y, Vegas AJ, Alabi CA, Sahay G, Olejnik KT, Wang W, Schroeder A, Lytton- Jean AK, Siegwart DJ, Akinc A, Barnes C, Barros SA, Carioto M, Fitzgerald K, Hettinger J, Kumar V, Novobrantseva TI, Qin J, Querbes W, Koteliansky V, Langer R, Anderson DG. Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates. Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):3955-60. doi: 10.1073/pnas.1322937111. Epub 2014 Feb 10. Erratum in: Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5753. PMID: 24516150; PMCID: PMC3964096.