Synonym
DMRIE; dimyristyloxypropyl-3-dimethyl-hydroxyethyl ammonium;
IUPAC/Chemical Name
N-(2-hydroxyethyl)-N,N-dimethyl-2,3-bis(tetradecyloxy)propan-1-aminium bromide
InChi Key
WALUVDCNGPQPOD-UHFFFAOYSA-M
InChi Code
InChI=1S/C35H74NO3.BrH/c1-5-7-9-11-13-15-17-19-21-23-25-27-31-38-34-35(33-36(3,4)29-30-37)39-32-28-26-24-22-20-18-16-14-12-10-8-6-2;/h35,37H,5-34H2,1-4H3;1H/q+1;/p-1
SMILES Code
OCC[N+](C)(CC(COCCCCCCCCCCCCCC)OCCCCCCCCCCCCCC)C.[Br-]
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
636.89
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Kim BK, Doh KO, Hwang GB, Seu YB. Transfection property of a new cholesterol- based cationic lipid containing tri-2-hydroxyethylamine as gene delivery vehicle. J Microbiol Biotechnol. 2012 Jun;22(6):866-71. doi: 10.4014/jmb.1111.11010. PMID: 22573166.
2: Li XL, Lei Y, Zhong S, Peng FY, Zhou Z, Li K, Ren H. [Construction and functional characterization of a monocistronic replicon based on the HCV genotype 2a promotor]. Zhonghua Gan Zang Bing Za Zhi. 2012 Feb;20(2):103-7. Chinese. doi: 10.3760/cma.j.issn.1007-3418.2012.02.007. PMID: 22464780.
3: Lee SJ, Yoon SH, Doh KO. Enhancement of gene delivery using novel homodimeric tat peptide formed by disulfide bond. J Microbiol Biotechnol. 2011 Aug;21(8):802-7. doi: 10.4014/jmb.1105.05041. PMID: 21876369.
4: Goepfert C, Gazdhar A, Frey FJ, Frey BM. Effect of electroporation-mediated diphtheria toxin A expression on PSA positive human prostate xenograft tumors in SCID mice. Prostate. 2011 Jun 1;71(8):872-80. doi: 10.1002/pros.21303. Epub 2010 Nov 4. PMID: 21456069.
5: Wong AW, Baginski TK, Reilly DE. Enhancement of DNA uptake in FUT8-deleted CHO cells for transient production of afucosylated antibodies. Biotechnol Bioeng. 2010 Aug 1;106(5):751-63. doi: 10.1002/bit.22749. PMID: 20564613.
6: Branski LK, Masters OE, Herndon DN, Mittermayr R, Redl H, Traber DL, Cox RA, Kita K, Jeschke MG. Pre-clinical evaluation of liposomal gene transfer to improve dermal and epidermal regeneration. Gene Ther. 2010 Jun;17(6):770-8. doi: 10.1038/gt.2010.32. Epub 2010 Apr 8. PMID: 20376099.
7: Rhinn H, Largeau C, Bigey P, Kuen RL, Richard M, Scherman D, Escriou V. How to make siRNA lipoplexes efficient? Add a DNA cargo. Biochim Biophys Acta. 2009 Apr;1790(4):219-30. doi: 10.1016/j.bbagen.2009.01.005. PMID: 19714863.
8: Gong FY, Deng JY, Shi YF. Mek and p38 MAPK-dependant pathways are involoved in the positive effect of interleukin-6 on human growth hormone gene expression in rat MtT/S somatotroph cells. Chin Med Sci J. 2008 Jun;23(2):73-80. doi: 10.1016/s1001-9294(09)60016-x. PMID: 18686625.
9: Johler SM, Rejman J, Guan S, Rosenecker J. Nebulisation of IVT mRNA Complexes for Intrapulmonary Administration. PLoS One. 2015 Sep 9;10(9):e0137504. doi: 10.1371/journal.pone.0137504. PMID: 26352268; PMCID: PMC4564175.