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MedKoo Cat#: 464496 | Name: BCIG (cyclohexylammonium salt hydrate)
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

BCIG (X-GLUC) is a chromogenic substrate for β-glucuronidase. Upon cleavage by β-glucuronidase, an indigo pigment is released that can be used as a marker of β-glucuronidase activity. BCIG has been used for the detection of E. coli contamination in foodstuffs.

Chemical Structure

BCIG (cyclohexylammonium salt hydrate)
BCIG (cyclohexylammonium salt hydrate)
CAS#unknown

Theoretical Analysis

MedKoo Cat#: 464496

Name: BCIG (cyclohexylammonium salt hydrate)

CAS#: unknown

Chemical Formula: C20H28BrClN2O8

Exact Mass: 0.0000

Molecular Weight: 539.80

Elemental Analysis: C, 44.50; H, 5.23; Br, 14.80; Cl, 6.57; N, 5.19; O, 23.71

Price and Availability

Size Price Availability Quantity
10mg USD 350.00 2 Weeks
25mg USD 650.00 2 Weeks
50mg USD 950.00 2 Weeks
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Related CAS #
No Data
Synonym
BCIG; X-GLUC; XGLUC; X GLUC; 5-Bromo-4-chloro-3-indolyl-β-D-Glucuronide (cyclohexylammonium salt hydrate);
IUPAC/Chemical Name
cyclohexanamine (2S,3S,4S,5R,6S)-6-((5-bromo-4-chloro-1H-indol-3-yl)oxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylate hydrate
InChi Key
OMJGCRHKCXJGSX-ZSRZBWJVSA-N
InChi Code
InChI=1S/C14H13BrClNO7.C6H13N.H2O/c15-4-1-2-5-7(8(4)16)6(3-17-5)23-14-11(20)9(18)10(19)12(24-14)13(21)22;7-6-4-2-1-3-5-6;/h1-3,9-12,14,17-20H,(H,21,22);6H,1-5,7H2;1H2/t9-,10-,11+,12-,14+;;/m0../s1
SMILES Code
NC1CCCCC1.BrC2=CC=C3C(C(O[C@@H]4O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]4O)=CN3)=C2Cl.O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
To be determined
Shelf Life
>2 years if stored properly
Drug Formulation
To be determined
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Solvent mg/mL mM
Solubility
DMF 10.0 18.00
DMSO 10.0 18.00
Ethanol 0.5 0.93
PBS (pH 7.2) 1.0 1.80
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 539.80 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
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PMID: 22431969; PMCID: PMC3303778. 11: Choi YE, Lim S, Kim HJ, Han JY, Lee MH, Yang Y, Kim JA, Kim YS. Tobacco NtLTP1, a glandular-specific lipid transfer protein, is required for lipid secretion from glandular trichomes. Plant J. 2012 May;70(3):480-91. doi: 10.1111/j.1365-313X.2011.04886.x. Epub 2012 Feb 6. PMID: 22171964. 12: Xiong AS, Peng RH, Zhuang J, Chen JM, Zhang B, Zhang J, Yao QH. A thermostable β-glucuronidase obtained by directed evolution as a reporter gene in transgenic plants. PLoS One. 2011;6(11):e26773. doi: 10.1371/journal.pone.0026773. Epub 2011 Nov 9. PMID: 22096498; PMCID: PMC3212524. 13: Peto A, Lehotai N, Lozano-Juste J, León J, Tari I, Erdei L, Kolbert Z. Involvement of nitric oxide and auxin in signal transduction of copper-induced morphological responses in Arabidopsis seedlings. Ann Bot. 2011 Sep;108(3):449-57. doi: 10.1093/aob/mcr176. PMID: 21856638; PMCID: PMC3158692. 14: Tu Z, Zhang J, Yang G, He G. 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