PIPES monosodium | CAS#10010-67-0 | Buffering agent

MedKoo Cat#: 464428 | Name: PIPES monosodium

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

PIPES is frequently used as a buffering agent in biochemistry. It is useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.

Chemical Structure

PIPES monosodium

CAS#10010-67-0

Theoretical Analysis

MedKoo Cat#: 464428

Name: PIPES monosodium

CAS#: 10010-67-0

Chemical Formula: C8H17N2NaO6S2

Exact Mass:

Molecular Weight: 324.34

Elemental Analysis: C, 29.63; H, 5.28; N, 8.64; Na, 7.09; O, 29.60; S, 19.77

Price and Availability

Size	Price	Availability
25g	USD 350.00	2 Weeks
100g	USD 550.00	2 Weeks
500g	USD 1,650.00	2 Weeks

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Related CAS #

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Synonym

PIPES monosodium;

IUPAC/Chemical Name

sodium 2-(4-(2-sulfoethyl)piperazin-1-yl)ethane-1-sulfonate

InChi Key

OGGAIRCLBMGXCZ-UHFFFAOYSA-M

InChi Code

InChI=1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1

SMILES Code

OS(=O)(CCN1CCN(CC1)CCS(=O)([O-])=O)=O.[Na+]

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

To be determined

Shelf Life

>2 years if stored properly

Drug Formulation

To be determined

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

PIPES is frequently used as a buffering agent in biochemistry.

In vitro activity:

The incubation of MP (myofibrillar protein) suspensions (20 mg mL-1 in piperazine-N,N'-bis(2-ethanesulfonic acid) buffer, with 0.6 mol L-1 sodium chloride, pH 6.25) at 4 °C for 24 h under an iron-catalyzed hydroxyl radical generating system (Fenton reaction) promoted the formation of thiobarbituric acid reactive substances and protein carbonyls, which was attenuated by EC (5, 50, and 100 μmol g-1 protein). Reduced protein sulfhydryl content, tryptophan fluorescence, protein solubility, as well as increased surface hydrophobicity were found by the co-incubation of EC. Analysis by scanning electron microscopy revealed increased protein aggregation and fragments in oxidized MP, which were further enhanced by the addition of EC. However, the protein digestibility of MP was not affected. Reference: J Sci Food Agric. 2021 Jan 30;101(2):746-753. https://pubmed.ncbi.nlm.nih.gov/32706121/

In vivo activity:

This study found that piperazine-N,N'-bis(ethanesulfonic acid) (PIPES) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), two zwitterionic buffers commonly used in tissue fixation, can cause severe lumen and cell morphological defects in Drosophila pupal and adult retina; the inter-rhabdomeral lumen becomes dilated and the photoreceptor cells are significantly reduced in size. Correspondingly, the localization pattern of Eyes shut (EYS), a luminal protein, is severely altered. In contrast, tissues fixed in the phosphate buffered saline (PBS) buffer results in lumen and cell morphologies that are consistent with live imaging. Reference: BMC Dev Biol. 2015 Feb 3;15:10. https://pubmed.ncbi.nlm.nih.gov/25645690/

	Solvent	mg/mL	mM
Solubility
	0.1M NaOH	250.0	0.00

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 324.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Xu M, Sun M, Lu C, Han Y, Yao X, Niu X, Xu M, Zhu Q. Influence of epicatechin on oxidation-induced physicochemical and digestibility changes in porcine myofibrillar proteins during refrigerated storage. J Sci Food Agric. 2021 Jan 30;101(2):746-753. doi: 10.1002/jsfa.10687. Epub 2020 Aug 19. PMID: 32706121. 2. Hugel S, Kadiri N, Rodeau JL, Gaillard S, Schlichter R. pH-dependent inhibition of native GABA(A) receptors by HEPES. Br J Pharmacol. 2012 Aug;166(8):2402-16. doi: 10.1111/j.1476-5381.2012.01956.x. PMID: 22452286; PMCID: PMC3448902. 3. Nie J, Mahato S, Zelhof AC. Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation. BMC Dev Biol. 2015 Feb 3;15:10. doi: 10.1186/s12861-015-0056-y. PMID: 25645690; PMCID: PMC4320506.

In vitro protocol:

In vivo protocol:

1. Nie J, Mahato S, Zelhof AC. Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation. BMC Dev Biol. 2015 Feb 3;15:10. doi: 10.1186/s12861-015-0056-y. PMID: 25645690; PMCID: PMC4320506.

1: Arakawa T, Timasheff SN. The mechanism of action of Na glutamate, lysine HCl, and piperazine-N,N'-bis(2-ethanesulfonic acid) in the stabilization of tubulin and microtubule formation. J Biol Chem. 1984 Apr 25;259(8):4979-86. PMID: 6325414. 2: Roy RN, Gibbons JJ, Padron JL, Buechter K, Faszholz S, Doan H. Revised values of the paH of monosodium 1,4-piperazinediethanesulfonate ("Pipes') in water and other buffers in isotonic saline at various temperatures. Clin Chem. 1981 Oct;27(10):1787-8. PMID: 6269781.