MedKoo Cat#: 464074 | Name: Bromcresol green
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Bromocresol Green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualisation of the compounds with functional groups whose pKa is below 5.0.

Chemical Structure

Bromcresol green
Bromcresol green
CAS#76-60-8

Theoretical Analysis

MedKoo Cat#: 464074

Name: Bromcresol green

CAS#: 76-60-8

Chemical Formula: C21H14Br4O5S

Exact Mass: 693.7295

Molecular Weight: 698.01

Elemental Analysis: C, 36.14; H, 2.02; Br, 45.79; O, 11.46; S, 4.59

Price and Availability

Size Price Availability Quantity
5g USD 350.00 2 Weeks
25g USD 650.00 2 Weeks
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Related CAS #
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Synonym
Bromcresol green; BCG; NSC 7817; NSC7817; NSC-7817;
IUPAC/Chemical Name
3,3-bis(3,5-dibromo-4-hydroxy-2-methylphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide
InChi Key
FRPHFZCDPYBUAU-UHFFFAOYSA-N
InChi Code
InChI=1S/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3
SMILES Code
CC1=C(Br)C(O)=C(Br)C=C1C2(C3=CC(Br)=C(O)C(Br)=C3C)C4=CC=CC=C4S(O2)(=O)=O
Appearance
Solid powder
Purity
Dye content 95%
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Solvent mg/mL mM
Solubility
Soluble in DMSO 0.0 100.00
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 698.01 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Glazunova OA, Moiseenko KV, Savinova OS, Fedorova TV. Purification and Characterization of Two Novel Laccases from Peniophora lycii. J Fungi (Basel). 2020 Dec 6;6(4):340. doi: 10.3390/jof6040340. PMID: 33291231; PMCID: PMC7762197. 2: Yao XY, Wu YF, Gao MC, Hong RH, Ding J, Hao Y, Zhang Y, Guan YT. Serum albumin level is associated with the severity of neurological dysfunction of NMOSD patients. Mult Scler Relat Disord. 2020 Aug;43:102130. doi: 10.1016/j.msard.2020.102130. Epub 2020 Apr 28. PMID: 32417662. 3: De Angelis G, Menchinelli G, Torelli R, De Carolis E, Posteraro P, Sanguinetti M, Posteraro B. Different detection capabilities by mycological media for Candida isolates from mono- or dual-species cultures. PLoS One. 2020 Mar 23;15(3):e0226467. doi: 10.1371/journal.pone.0226467. PMID: 32203515; PMCID: PMC7089522. 4: Hooijberg EH, Cray C, Miller M, Buss P, Steenkamp G, Goddard A. Bias between two methods of albumin measurement in the white rhinoceros, Ceratotherium simum. Vet Clin Pathol. 2020 Mar;49(1):91-94. doi: 10.1111/vcp.12820. Epub 2020 Jan 10. PMID: 31925822. 5: Gadallah MI, Ali HRH, Askal HF, Saleh GA. Poly (bromocresol green) flakes- decorated pencil graphite electrode for selective electrochemical sensing applications and pharmacokinetic studies. Mater Sci Eng C Mater Biol Appl. 2019 Sep;102:634-645. doi: 10.1016/j.msec.2019.03.071. Epub 2019 Mar 23. PMID: 31147035. 6: van de Logt AE, Rijpma SR, Vink CH, Prudon-Rosmulder E, Wetzels JF, van Berkel M. The bias between different albumin assays may affect clinical decision-making. Kidney Int. 2019 Jun;95(6):1514-1517. doi: 10.1016/j.kint.2019.01.042. Epub 2019 Feb 27. PMID: 31053386. 7: Promphet N, Rattanawaleedirojn P, Siralertmukul K, Soatthiyanon N, Potiyaraj P, Thanawattano C, Hinestroza JP, Rodthongkum N. Non-invasive textile based colorimetric sensor for the simultaneous detection of sweat pH and lactate. Talanta. 2019 Jan 15;192:424-430. doi: 10.1016/j.talanta.2018.09.086. Epub 2018 Sep 24. PMID: 30348413. 8: Chaiyo S , Kalcher K , Apilux A , Chailapakul O , Siangproh W . A novel paper-based colorimetry device for the determination of the albumin to creatinine ratio. Analyst. 2018 Nov 5;143(22):5453-5460. doi: 10.1039/c8an01122b. PMID: 30318543. 9: Osuntokun J, Onwudiwe DC, Ebenso EE. Aqueous extract of broccoli mediated synthesis of CaO nanoparticles and its application in the photocatalytic degradation of bromocrescol green. IET Nanobiotechnol. 2018 Oct;12(7):888-894. doi: 10.1049/iet-nbt.2017.0277. PMID: 30247126. 10: de Roij van Zuijdewijn CLM, de Haseth DE, van Dam B, Bax WA, Grooteman MPC, Bots ML, Blankestijn PJ, Nubé MJ, van den Dorpel MA, Ter Wee PM, Penne EL. Role of Albumin Assay on Calcium Levels and Prescription of Phosphate Binders in Chronic Hemodialysis Patients. Nephron. 2018;140(3):211-217. doi: 10.1159/000492238. Epub 2018 Sep 13. PMID: 30212837; PMCID: PMC6262677. 11: Garcia Moreira V, Beridze Vaktangova N, Martinez Gago MD, Laborda Gonzalez B, Garcia Alonso S, Fernandez Rodriguez E. Overestimation of Albumin Measured by Bromocresol Green vs Bromocresol Purple Method: Influence of Acute-Phase Globulins. Lab Med. 2018 Oct 11;49(4):355-361. doi: 10.1093/labmed/lmy020. PMID: 29790973. 12: Chun H. Electropreconcentration-induced local pH change. Electrophoresis. 2018 Feb;39(3):521-525. doi: 10.1002/elps.201700373. Epub 2017 Nov 24. PMID: 29077205. 13: Delanghe S, Biesen WV, Velde NV, Eloot S, Pletinck A, Schepers E, Glorieux G, Delanghe JR, Speeckaert MM. Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients. Clin Chem Lab Med. 2018 Feb 23;56(3):436-440. doi: 10.1515/cclm-2017-0444. PMID: 28985181. 14: Pan WC, Lau W, Mattman A, Kiaii M, Jung B. Comparison of hypoalbuminemia- corrected serum calcium using BCP albumin assay to ionized calcium and impact on prescribing in hemodialysis patients. Clin Nephrol. 2018 Jan;89 (2018)(1):34-40. doi: 10.5414/CN109070. PMID: 28853704. 15: Christensen PA. Reference intervals for the P-Albumin bromocresol purple method. Scand J Clin Lab Invest. 2017 Oct;77(6):472-476. doi: 10.1080/00365513.2017.1337217. Epub 2017 Jun 14. PMID: 28613959. 16: Rossary A, Blondé-Cynober F, Bastard JP, Beauvieux MC, Beyne P, Drai J, Lombard C, Anglard I, Aussel C, Claeyssens S, Vasson MP. Determination of serum albumin, analytical challenges: a French multicenter study. Ann Biol Clin (Paris). 2017 Jun 1;75(3):305-318. English. doi: 10.1684/abc.2017.1239. PMID: 28540853. 17: Hegyi T, Kleinfeld A, Huber A, Weinberger B, Memon N, Shih WJ, Carayannopoulos M, Oh W. Effects of Soybean Lipid Infusion on Unbound Free Fatty Acids and Unbound Bilirubin in Preterm Infants. J Pediatr. 2017 May;184:45-50.e1. doi: 10.1016/j.jpeds.2016.12.026. Epub 2017 Jan 17. PMID: 28108102; PMCID: PMC5403563. 18: Bachmann LM, Yu M, Boyd JC, Bruns DE, Miller WG. State of Harmonization of 24 Serum Albumin Measurement Procedures and Implications for Medical Decisions. Clin Chem. 2017 Mar;63(3):770-779. doi: 10.1373/clinchem.2016.262899. Epub 2017 Jan 10. PMID: 28073902. 19: Bai H, He P, Chen J, Liu K, Lei H, Zhang X, Dong F, Li H. Electrocatalytic degradation of bromocresol green wastewater on Ti/SnO2-RuO2 electrode. Water Sci Technol. 2017 Jan;75(1-2):220-227. doi: 10.2166/wst.2016.509. PMID: 28067662. 20: Chmelová D, Ondrejovič M. Purification and characterization of extracellular laccase produced by Ceriporiopsis subvermispora and decolorization of triphenylmethane dyes. J Basic Microbiol. 2016 Nov;56(11):1173-1182. doi: 10.1002/jobm.201600152. Epub 2016 Aug 31. PMID: 27577103.