Bt354 is a novel STAT3 Signaling Pathway Inhibitor against Triple Negative Breast Cancer. Bt354 significantly inhibited STAT3 activity in a dual luciferase assay. Bt354 inhibited the proliferation of cancer cells in a dose- and time-dependent manner. The phosphorylation of STAT3 at Y705 was suppressed without affecting the phosphorylation of STAT3 at S727 in breast cancer cells. Furthermore, Bt354 inhibited the nuclear translocation of STAT3 and consequently induced cell growth inhibition, apoptosis and cell cycle G2/M arrest in triple negative breast cancer cells. In vivo, Bt354 also inhibited tumour growth in MDA-MB-435 and MDA-MB-231 xenograft mice without affecting body weight. Computational modelling showed that Bt354 could bind to the SH2 domain of STAT3. These findings suggest that Bt354 may be a potent anticancer agent for STAT3-activated triple negative breast cancer cells.
MedKoo Cat#: 463446
Name: Bt354
CAS#: 931305-29-2
Chemical Formula: C21H21N3O3S
Exact Mass: 395.1304
Molecular Weight: 395.48
Elemental Analysis: C, 63.78; H, 5.35; N, 10.63; O, 12.14; S, 8.11
The following data is based on the product molecular weight 395.48 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
