Polysorbate 20 | CAS# 9005-64-5 | emulsifiers or dispersing agents in pharmaceuticals

MedKoo Cat#: 412277 | Name: Polysorbate 20

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Polysorbate 20, sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharmaceuticals. Polysorbate 20 (common commercial brand names include Scattics, Alkest TW 20 and Tween 20) is a polysorbate-type nonionic surfactant formed by the ethoxylation of sorbitan before the addition of lauric acid. Its stability and relative nontoxicity allows it to be used as a detergent and emulsifier in a number of domestic, scientific, and pharmacological applications. As the name implies the ethoxylation process leaves the molecule with 20 repeat units of polyethylene glycol; in practice these are distributed across 4 different chains, leading to a commercial product containing a range of chemical species.

Chemical Structure

Polysorbate 20

CAS#9005-64-5

Theoretical Analysis

MedKoo Cat#: 412277

Name: Polysorbate 20

CAS#: 9005-64-5

Chemical Formula: C26H50O10

Exact Mass: 522.3404

Molecular Weight: 522.68

Elemental Analysis: C, 59.75; H, 9.64; O, 30.61

Price and Availability

Size	Price	Availability	Quantity
10g	USD 246.20	2 Weeks
60g	USD 496.00	2 Weeks

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Related CAS #

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Synonym

Polysorbate 20; CCRIS699; CCRIS-699; CCRIS 699

IUPAC/Chemical Name

2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate

InChi Key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

InChi Code

InChI=1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

SMILES Code

CCCCCCCCCCCC(OCCOCC(C1OCC(OCCO)C1OCCO)OCCO)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

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Product Data

Product Data

Instruction

View handling instruction

Biological target:

Polysorbate 20 is a polyoxyethylene (POE)-type nonionic surfactant.

In vitro activity:

The amorphous formulations were created by different freeze-drying processes only differing in their freezing step (random nucleation; additional annealing step of 1.5 h and 3 h, controlled nucleation; quench cooling). Samples that crystallized during the study time of 12 months showed a promising correlation between their relaxation time and crystallization behavior upon storage. Furthermore, the study shows that polysorbate 20 strongly accelerates crystallization of sucrose and that the freezing step itself has a strong impact on the relaxation phenomena that is not levelled out by primary and secondary drying. Reference: Pharmaceutics. 2023 Feb 20;15(2):703. https://pubmed.ncbi.nlm.nih.gov/36840026/

In vivo activity:

The results showed an increased absorptive transport and a reduced secretory transport in MDCKII-MDR and Caco-2 cells as a function of polysorbate 20 concentrations. The secretory transport (B-A) of digoxin was reduced by 50% at lower polysorbate 20 concentrations than required to increase the absorptive transport (A-B). In vivo, the oral bioavailability of digoxin in wild type animal was increased by 10-25% (w/v) polysorbate 20. In mdr1a deficient Sprague Dawley rats 25% (w/v) polysorbate 20 did not alter the absorption of digoxin after oral administration, but digoxin exposure was significantly different between wild type and mdr1a deficient rats. In conclusion, polysorbate 20 increased absorptive transport across Caco-2 cell monolayers and in vivo in rats in a concentration dependent manner, most likely via inhibition of P-gp rather than through solubilization of digoxin. Reference: Int J Pharm. 2016 Nov 20;513(1-2):78-87. https://pubmed.ncbi.nlm.nih.gov/27601334/

	Solvent	mg/mL	mM	comments
Solubility
	DMSO	50.0	95.66

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 522.68 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Groël S, Menzen T, Winter G. Prediction of Unwanted Crystallization of Freeze-Dried Protein Formulations Using α-Relaxation Measurements. Pharmaceutics. 2023 Feb 20;15(2):703. doi: 10.3390/pharmaceutics15020703. PMID: 36840026; PMCID: PMC9963158. 2. Chen YA, Zhou Y, Qin Y, Liu D, Zhao X. Evaluation of the action of Tween 20 non-ionic surfactant during enzymatic hydrolysis of lignocellulose: Pretreatment, hydrolysis conditions and lignin structure. Bioresour Technol. 2018 Dec;269:329-338. doi: 10.1016/j.biortech.2018.08.119. Epub 2018 Aug 30. PMID: 30195225. 3. Nielsen CU, Abdulhussein AA, Colak D, Holm R. Polysorbate 20 increases oral absorption of digoxin in wild-type Sprague Dawley rats, but not in mdr1a(-/-) Sprague Dawley rats. Int J Pharm. 2016 Nov 20;513(1-2):78-87. doi: 10.1016/j.ijpharm.2016.09.011. Epub 2016 Sep 4. PMID: 27601334.

In vitro protocol:

In vivo protocol:

1. Nielsen CU, Abdulhussein AA, Colak D, Holm R. Polysorbate 20 increases oral absorption of digoxin in wild-type Sprague Dawley rats, but not in mdr1a(-/-) Sprague Dawley rats. Int J Pharm. 2016 Nov 20;513(1-2):78-87. doi: 10.1016/j.ijpharm.2016.09.011. Epub 2016 Sep 4. PMID: 27601334.

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