Polysorbate 80 | CAS#9005-65-6

MedKoo Cat#: 462695 | Name: Polysorbate 80

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Polysorbate 80 is a nonionic surfactant and emulsifier often used in foods and cosmetics. This synthetic compound is a viscous, water-soluble yellow liquid.

Chemical Structure

Polysorbate 80

CAS#9005-65-6

Theoretical Analysis

MedKoo Cat#: 462695

Name: Polysorbate 80

CAS#: 9005-65-6

Chemical Formula: C32H60O10

Exact Mass: 604.4186

Molecular Weight: 604.82

Elemental Analysis: C, 63.55; H, 10.00; O, 26.45

Price and Availability

Size	Price	Availability	Quantity
100g	USD 210.00	2 Weeks
1kg	USD 410.00	2 Weeks

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Related CAS #

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Synonym

Tween 80; Polysorbate 80;

IUPAC/Chemical Name

2-(2-(3,5-bis(2-hydroxyethoxy)tetrahydrofuran-2-yl)-2-(2-hydroxyethoxy)ethoxy)ethyl (E)-octadec-9-enoate

InChi Key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

InChi Code

InChI=1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

SMILES Code

CCCCCCCC/C=C/CCCCCCCC(OCCOCC(OCCO)C1C(OCCO)CC(OCCO)O1)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

Polysorbate 80 is a nonionic surfactant and emulsifier often used in foods and cosmetics.

In vitro activity:

The effects of Tween 80 on the changes in the protein concentration of individual cellulase components were investigated in the absence of substrates. Agitation of the enzyme solution resulted in the drastic decrease of SDS-PAGE bands intensity of CBH2 (cellobiohydrolase 2). The addition of Tween 80 prevented this. Thus, the Tween 80 functions to stabilize instable cellulase components under the agitated condition. Moreover, addition of Tween 80 completely suppressed the decrease of CBH2 intensity by agitation at 30°C. Results suggest that Tween 80 stabilizes instable cellulase components not only during hydrolysis, but during enzyme production also. Reference: Bioresour Technol. 2013 Aug;142:535-9. https://pubmed.ncbi.nlm.nih.gov/23765004/

In vivo activity:

First of all, this study verified that P80 (polysorbate 80) promoted the bioavailability of MEHP-AF in the long-term and low-dose exposure of MEHP-AF with P80 as a result of increasing the intestinal absorption of MEHP-AF in rats. Afterwards, experimental results from Western blot, qPCR, immunohistochemistry, and immunofluorescence showed that P80 decreased the expression of proteins (mucus protein mucin-2, tight junction proteins claudin-1 and occludin) related to mucus barrier and mucosal barrier in the intestine, changed the integrity of intestinal epithelial cell, and increased the permeability of intestinal epithelial mucosa. These results indicated that P80 promoted the oral absorption of MEHP-AF by altering the intestinal mucus barrier and mucosal barrier. Reference: Toxicol Appl Pharmacol. 2021 Mar 1;414:115411. https://pubmed.ncbi.nlm.nih.gov/33476678/

Preparing Stock Solutions

The following data is based on the product molecular weight 604.82 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Kim RE, Choi JS. Polysorbate 80 blocked a peripheral sodium channel, Nav1.7, and reduced neuronal excitability. Mol Pain. 2023 Jan-Dec;19:17448069221150138. doi: 10.1177/17448069221150138. PMID: 36550597; PMCID: PMC9829885. 2. Okino S, Ikeo M, Ueno Y, Taneda D. Effects of Tween 80 on cellulase stability under agitated conditions. Bioresour Technol. 2013 Aug;142:535-9. doi: 10.1016/j.biortech.2013.05.078. Epub 2013 May 27. PMID: 23765004. 3. Zhu YT, Yuan YZ, Feng QP, Hu MY, Li WJ, Wu X, Xiang SY, Yu SQ. Food emulsifier polysorbate 80 promotes the intestinal absorption of mono-2-ethylhexyl phthalate by disturbing intestinal barrier. Toxicol Appl Pharmacol. 2021 Mar 1;414:115411. doi: 10.1016/j.taap.2021.115411. Epub 2021 Jan 18. PMID: 33476678. 4. Nishimura S, Aoi W, Kodani H, Kobayashi Y, Wada S, Kuwahata M, Higashi A. Polysorbate 80-induced leaky gut impairs skeletal muscle metabolism in mice. Physiol Rep. 2020 Oct;8(20):e14629. doi: 10.14814/phy2.14629. PMID: 33113283; PMCID: PMC7592879.

In vitro protocol:

In vivo protocol:

1. Zhu YT, Yuan YZ, Feng QP, Hu MY, Li WJ, Wu X, Xiang SY, Yu SQ. Food emulsifier polysorbate 80 promotes the intestinal absorption of mono-2-ethylhexyl phthalate by disturbing intestinal barrier. Toxicol Appl Pharmacol. 2021 Mar 1;414:115411. doi: 10.1016/j.taap.2021.115411. Epub 2021 Jan 18. PMID: 33476678. 2. Nishimura S, Aoi W, Kodani H, Kobayashi Y, Wada S, Kuwahata M, Higashi A. Polysorbate 80-induced leaky gut impairs skeletal muscle metabolism in mice. Physiol Rep. 2020 Oct;8(20):e14629. doi: 10.14814/phy2.14629. PMID: 33113283; PMCID: PMC7592879.

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