MedKoo Biosciences, Inc.
Tel:   +1-919-636-5577    Fax:   +1-919-980-4831    Email:   sales@medkoo.com
Search
Login Register
Search
MedKoo Cat#: 555829 | Name: LIN28 inhibitor LI71
Featured New

Description:

WARNING: This product is for research use only, not for human or veterinary use.

LIN28 inhibitor LI71 is a potent, cell-permeable inhibitor of LIN28 that abolishes LIN28-mediated oligouridylation. LIN28 inhibitor LI71 binds the cold shock domain to suppress LIN28's activity against let-7 in leukemia cells and embryonic stem cells.

Chemical Structure

LIN28 inhibitor LI71
LIN28 inhibitor LI71
CAS#1357248-83-9

Theoretical Analysis

MedKoo Cat#: 555829

Name: LIN28 inhibitor LI71

CAS#: 1357248-83-9

Chemical Formula: C21H21NO3

Exact Mass: 335.1521

Molecular Weight: 335.40

Elemental Analysis: C, 75.20; H, 6.31; N, 4.18; O, 14.31

Price and Availability

Size Price Availability Quantity
100mg USD 2,950.00 2 Weeks
200mg USD 3,950.00 2 Weeks
500mg USD 5,950.00 2 Weeks
1g USD 8,950.00 2 Weeks
2g USD 16,950.00 2 Weeks
Bulk Inquiry
Buy Now
Add to Cart
Related CAS #
No Data
Synonym
LIN28 inhibitor LI71; L-I71; L I71;
IUPAC/Chemical Name
4-((3aS,4R,9bR)-6-ethoxy-3a,4,5,9b-tetrahydro-3H-cyclopenta[c]quinolin-4-yl)benzoic acid
InChi Key
QWJMABCFVYELBB-GJYPPUQNSA-N
InChi Code
InChI=1S/C21H21NO3/c1-2-25-18-8-4-7-17-15-5-3-6-16(15)19(22-20(17)18)13-9-11-14(12-10-13)21(23)24/h3-5,7-12,15-16,19,22H,2,6H2,1H3,(H,23,24)/t15-,16+,19+/m1/s1
SMILES Code
O=C(O)C1=CC=C([C@@H]2NC3=C(C=CC=C3OCC)[C@@]4([H])[C@]2([H])CC=C4)C=C1
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
LIN28 inhibitor LI71 is a potent and cell-permeable LIN28 inhibitor, which abolishes LIN28-mediated oligouridylation with an IC50 of 7 uM.
In vitro activity:
LI71 could significantly reduce the relative Renilla luciferase activity in cells expressing LIN28A and LIN28B at 50–100 μM (Figures 6B and 6C). Together, these results demonstrate that, consistent with in vitro data, LI71 inhibits LIN28’s activity on let-7 micro-RNAs in LIN28-dependent cancer and embryonic stem cells. Reference: Cell Rep. 2018 Jun 5;23(10):3091-3101. https://pubmed.ncbi.nlm.nih.gov/29874593/
In vivo activity:
TBD
Solvent mg/mL mM comments
Solubility
DMSO 100.0 298.15
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 335.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Wang L, Rowe RG, Jaimes A, Yu C, Nam Y, Pearson DS, Zhang J, Xie X, Marion W, Heffron GJ, Daley GQ, Sliz P. Small-Molecule Inhibitors Disrupt let-7 Oligouridylation and Release the Selective Blockade of let-7 Processing by LIN28. Cell Rep. 2018 Jun 5;23(10):3091-3101. doi: 10.1016/j.celrep.2018.04.116. PMID: 29874593; PMCID: PMC6511231.
In vitro protocol:
1. Wang L, Rowe RG, Jaimes A, Yu C, Nam Y, Pearson DS, Zhang J, Xie X, Marion W, Heffron GJ, Daley GQ, Sliz P. Small-Molecule Inhibitors Disrupt let-7 Oligouridylation and Release the Selective Blockade of let-7 Processing by LIN28. Cell Rep. 2018 Jun 5;23(10):3091-3101. doi: 10.1016/j.celrep.2018.04.116. PMID: 29874593; PMCID: PMC6511231.
In vivo protocol:
TBD
Wang L, Rowe RG, Jaimes A, Yu C, Nam Y, Pearson DS, Zhang J, Xie X, Marion W, Heffron GJ, Daley GQ, Sliz P. Small-Molecule Inhibitors Disrupt let-7 Oligouridylation and Release the Selective Blockade of let-7 Processing by LIN28. Cell Rep. 2018 Jun 5;23(10):3091-3101. doi: 10.1016/j.celrep.2018.04.116. PMID: 29874593; PMCID: PMC6511231.