Synonym
Nbd-colcemid; Nbd colcemid; Nbdcolcemid
IUPAC/Chemical Name
Benzo(a)heptalen-9(5H)-one, 6,7-dihydro-1,2,3,10-tetramethoxy-7-(methyl(7-nitro-4-benzofurazanyl)amino)-, (S)-
InChi Key
DQARLXAFVVPTDP-KRWDZBQOSA-N
InChi Code
InChI=1S/C27H26N4O8/c1-30(18-9-10-19(31(33)34)25-24(18)28-39-29-25)17-8-6-14-12-22(36-3)26(37-4)27(38-5)23(14)15-7-11-21(35-2)20(32)13-16(15)17/h7,9-13,17H,6,8H2,1-5H3/t17-/m0/s1
SMILES Code
O=C1C=C2[C@@H](N(C)C3=CC=C([N+]([O-])=O)C4=NON=C34)CCC5=CC(OC)=C(OC)C(OC)=C5C2=CC=C1OC
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
534.53
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Hiratsuka T, Kato T. A fluorescent analog of colcemid, N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-colcemid, as a probe for the colcemid- binding sites of tubulin and microtubules. J Biol Chem. 1987 May 5;262(13):6318-22. PMID: 3571259.
2: Sengupta S, Mahapatra PK, Chakrabarti G, Roy S, Bhattacharyya B. Interaction of a fluorescent analog of N-deacetyl-N-methyl-colchicine (colcemid) with liver alcohol dehydrogenase. Eur J Biochem. 1995 Sep 15;232(3):844-8. PMID: 7588725.
3: Sengupta S, Puri KD, Surolia A, Roy S, Bhattacharyya B. N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)colcemid, a probe for different classes of colchicine-binding site on tubulin. Eur J Biochem. 1993 Mar 1;212(2):387-93. doi: 10.1111/j.1432-1033.1993.tb17673.x. Erratum in: Eur J Biochem 1993 Sep 15;216(3):879. PMID: 8444175.
4: Sengupta S, Banerjee S, Chakrabarti G, Mahapatra PK, Roy S, Bhattacharyya B. NBD-isocolcemid-tubulin interaction: a novel one-step reaction involving no conformational adjustment of reactants. Biochemistry. 2000 Mar 7;39(9):2227-34. doi: 10.1021/bi991530e. PMID: 10694388.
5: Bothra AK, Roy S, Bhattacharyya B, Mukhopadhyay C. Molecular dynamics simulation of colchicinoids. J Biomol Struct Dyn. 1998 Apr;15(5):999-1008. doi: 10.1080/07391102.1998.10508219. PMID: 9619520.
6: Sharma R, Lovell HB, Wiegmann TB, Savin VJ. Vasoactive substances induce cytoskeletal changes in cultured rat glomerular epithelial cells. J Am Soc Nephrol. 1992 Nov;3(5):1131-8. PMID: 1336407.
