Synonym
EB-3D; EB 3D; EB3D;
IUPAC/Chemical Name
1,1'-(((ethane-1,2-diylbis(oxy))bis(4,1-phenylene))bis(methylene))bis(4-(dimethylamino)pyridinium) bromide
InChi Key
SCXVGPSPZQBBDM-UHFFFAOYSA-L
InChi Code
InChI=1S/C30H36N4O2.2BrH/c1-31(2)27-13-17-33(18-14-27)23-25-5-9-29(10-6-25)35-21-22-36-30-11-7-26(8-12-30)24-34-19-15-28(16-20-34)32(3)4;;/h5-20H,21-24H2,1-4H3;2*1H/q+2;;/p-2
SMILES Code
CN(C1=CC=[N+](CC2=CC=C(OCCOC3=CC=C(C[N+]4=CC=C(N(C)C)C=C4)C=C3)C=C2)C=C1)C.[Br-].[Br-]
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
Choline kinase alpha 1 (ChoKα1) has recently become an interesting therapeutic target since its overexpression has been associated to tumorigenesis in many cancers.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
644.45
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Sola-Leyva A, López-Cara LC, Ríos-Marco P, Ríos A, Marco C, Carrasco-Jiménez
MP. Choline kinase inhibitors EB-3D and EB-3P interferes with lipid homeostasis
in HepG2 cells. Sci Rep. 2019 Mar 25;9(1):5109. doi: 10.1038/s41598-019-40885-z.
PMID: 30911014; PMCID: PMC6433853.
2: Mariotto E, Bortolozzi R, Volpin I, Carta D, Serafin V, Accordi B, Basso G,
Navarro PL, López-Cara LC, Viola G. EB-3D a novel choline kinase inhibitor
induces deregulation of the AMPK-mTOR pathway and apoptosis in leukemia T-cells.
Biochem Pharmacol. 2018 Sep;155:213-223. doi: 10.1016/j.bcp.2018.07.004. Epub
2018 Jul 11. PMID: 30006194.
3: Mariotto E, Viola G, Ronca R, Persano L, Aveic S, Bhujwalla ZM, Mori N,
Accordi B, Serafin V, López-Cara LC, Bortolozzi R. Choline Kinase Alpha
Inhibition by EB-3D Triggers Cellular Senescence, Reduces Tumor Growth and
Metastatic Dissemination in Breast Cancer. Cancers (Basel). 2018 Oct
22;10(10):391. doi: 10.3390/cancers10100391. PMID: 30360374; PMCID: PMC6209942.
4: Qu H, Han Z, Chen Z, Tang L, Gao C, Liu K, Pan H, Fu H, Ruan C. Fractal
Design Boosts Extrusion-Based 3D Printing of Bone-Mimicking Radial-Gradient
Scaffolds. Research (Wash D C). 2021 Nov 23;2021:9892689. doi:
10.34133/2021/9892689. PMID: 34909694; PMCID: PMC8637129.
