LDV | CAS# 1155866-55-9 | α4β1 integrin (VLA-4) ligand

MedKoo Cat#: 574068 | Name: LDV

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

LDV is an α4β1 integrin (VLA-4) ligand and non-fluorescent derivative of LDV FITC.

Chemical Structure

LDV

CAS#1155866-55-9

Theoretical Analysis

MedKoo Cat#: 574068

Name: LDV

CAS#: 1155866-55-9

Chemical Formula: C48H70N10O12

Exact Mass: 978.5175

Molecular Weight: 979.15

Elemental Analysis: C, 58.88; H, 7.21; N, 14.31; O, 19.61

Price and Availability

Size	Price	Availability	Quantity
1mg	USD 450.00	2 Weeks

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Synonym

LDV

IUPAC/Chemical Name

(2-(4-(3-(o-tolyl)ureido)phenyl)acetyl)-L-leucyl-L-aspartyl-L-valyl-L-prolyl-L-alanyl-L-alanyl-L-lysine

InChi Key

FQYZPSNPTJYQKB-CGTMUHGOSA-N

InChi Code

InChI=1S/C48H70N10O12/c1-26(2)23-35(53-38(59)24-31-17-19-32(20-18-31)52-48(70)56-33-14-9-8-13-28(33)5)43(64)55-36(25-39(60)61)44(65)57-40(27(3)4)46(67)58-22-12-16-37(58)45(66)51-29(6)41(62)50-30(7)42(63)54-34(47(68)69)15-10-11-21-49/h8-9,13-14,17-20,26-27,29-30,34-37,40H,10-12,15-16,21-25,49H2,1-7H3,(H,50,62)(H,51,66)(H,53,59)(H,54,63)(H,55,64)(H,57,65)(H,60,61)(H,68,69)(H2,52,56,70)/t29-,30-,34-,35-,36-,37-,40-/m0/s1

SMILES Code

CC(C)C[C@@H](C(N[C@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@H](C(N[C@H](C(N[C@H](C(O)=O)CCCCN)=O)C)=O)C)=O)=O)C(C)C)=O)CC(O)=O)=O)NC(CC(C=C2)=CC=C2NC(NC3=C(C)C=CC=C3)=O)=O

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

LDV is a α4β1 integrin (VLA-4) ligand, and binds α4β1 integrin in leukemia cells.

In vitro activity:

The tripeptide Leu-Asp-Val (LDV) is known to bind α(4)β(1) integrin in leukemia cells. Here this study has synthesized a LDV peptidomimetic equipped with a biotin-conjugated spacer-arm. Such biotin-conjugated LDV peptidomimetic may thus represent a novel tool for biotechnological applications using avidin interaction for leukapheresis or leukemia cell targeting. Reference: Bioorg Med Chem Lett. 2012 Jan 1;22(1):586-90. https://pubmed.ncbi.nlm.nih.gov/22101134/

In vivo activity:

Furthermore, YK3-LDV coating accelerated the in vivo formation of neointima-like tissue in a rat model with an ePTFE patch implanted into the carotid artery. Reference: Colloids Surf B Biointerfaces. 2022 Aug;216:112576. https://pubmed.ncbi.nlm.nih.gov/35636324/

	Solvent	mg/mL	mM
Solubility
	0.01M PBS (pH 7.4)	1.0	1.02

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 979.15 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Gérard E, Meulle A, Feron O, Marchand-Brynaert J. LDV peptidomimetics equipped with biotinylated spacer-arms: synthesis and biological evaluation on CCRF-CEM cell line. Bioorg Med Chem Lett. 2012 Jan 1;22(1):586-90. doi: 10.1016/j.bmcl.2011.10.078. Epub 2011 Oct 31. PMID: 22101134. 2. Njus BH, Chigaev A, Waller A, Wlodek D, Ostopovici-Halip L, Ursu O, Wang W, Oprea TI, Bologa CG, Sklar LA. Conformational mAb as a tool for integrin ligand discovery. Assay Drug Dev Technol. 2009 Oct;7(5):507-15. doi: 10.1089/adt.2009.0203. PMID: 19754304; PMCID: PMC3096548. 3. Kakinoki S, Nishioka S, Arichi Y, Yamaoka T. Stable and direct coating of fibronectin-derived Leu-Asp-Val peptide on ePTFE using one-pot tyrosine oxidation for endothelial cell adhesion. Colloids Surf B Biointerfaces. 2022 Aug;216:112576. doi: 10.1016/j.colsurfb.2022.112576. Epub 2022 May 24. PMID: 35636324. 4. De Marco R, Mazzotti G, Dattoli SD, Baiula M, Spampinato S, Greco A, Gentilucci L. 5-aminomethyloxazolidine-2,4-dione hybrid α/β-dipeptide scaffolds as inductors of constrained conformations: Applications to the synthesis of integrin antagonists. Biopolymers. 2015 Sep;104(5):636-49. doi: 10.1002/bip.22704. PMID: 26211418.

In vitro protocol:

In vivo protocol:

1. Kakinoki S, Nishioka S, Arichi Y, Yamaoka T. Stable and direct coating of fibronectin-derived Leu-Asp-Val peptide on ePTFE using one-pot tyrosine oxidation for endothelial cell adhesion. Colloids Surf B Biointerfaces. 2022 Aug;216:112576. doi: 10.1016/j.colsurfb.2022.112576. Epub 2022 May 24. PMID: 35636324. 2. De Marco R, Mazzotti G, Dattoli SD, Baiula M, Spampinato S, Greco A, Gentilucci L. 5-aminomethyloxazolidine-2,4-dione hybrid α/β-dipeptide scaffolds as inductors of constrained conformations: Applications to the synthesis of integrin antagonists. Biopolymers. 2015 Sep;104(5):636-49. doi: 10.1002/bip.22704. PMID: 26211418.

Chigaev et al (2009) Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation. J.Biol.Chem. 284 14337 PMID: 19251697