Mitapivat, also known as AG348, is a potent, selective allosteric activator of the enzyme pyruvate kinase (PK), specifically the red blood cell isoform PKR. By enhancing PKR activity, Mitapivat increases glycolytic flux, leading to improved ATP production and redox balance, which is particularly beneficial in conditions such as pyruvate kinase deficiency (PKD) and other hemolytic anemias.Mitapivat directly binds to PKR, increasing its enzymatic activity in purified enzyme systems and red blood cell lysates. It enhances the enzyme's affinity for PEP, boosting its catalytic efficiency. Treatment with Mitapivat in vitro leads to reductions in markers of oxidative stress in red blood cells.
MedKoo Cat#: 573466
Name: Mitapivat HCl
CAS#: 2559738-69-9 (HCl)
Chemical Formula: C24H27ClN4O3S
Exact Mass: 486.1492
Molecular Weight: 0.00
Elemental Analysis: C, 59.19; H, 5.59; Cl, 7.28; N, 11.50; O, 9.86; S, 6.58
The following data is based on the product molecular weight 0.00 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
