L48H37 | CAS# 343307-76-6 | MD2 Inhibitor

MedKoo Cat#: 564775 | Name: L48H37

Featured

Description:

WARNING: This product is for research use only, not for human or veterinary use.

L48H37 is a specific myeloid differentiation 2 (MD2) inhibitor.

Chemical Structure

L48H37

CAS#343307-76-6

Theoretical Analysis

MedKoo Cat#: 564775

Name: L48H37

CAS#: 343307-76-6

Chemical Formula: C27H33NO7

Exact Mass: 483.2257

Molecular Weight: 483.56

Elemental Analysis: C, 67.06; H, 6.88; N, 2.90; O, 23.16

Price and Availability

Size	Price	Availability
10mg	USD 320.00	2 Weeks
25mg	USD 550.00	2 Weeks
50mg	USD 850.00	2 Weeks

Bulk Inquiry

Buy Now

Add to Cart

Related CAS #

No Data

Synonym

L48-H37; L48 H37; L48H37

IUPAC/Chemical Name

1-Ethyl-3,5-bis(3,4,5-trimethoxybenzylidene)piperidin-4-one

InChi Key

IOFNKUXFKVPLPF-LQGKIZFRSA-N

InChi Code

InChI=1S/C27H33NO7/c1-8-28-15-19(9-17-11-21(30-2)26(34-6)22(12-17)31-3)25(29)20(16-28)10-18-13-23(32-4)27(35-7)24(14-18)33-5/h9-14H,8,15-16H2,1-7H3/b19-9+,20-10+

SMILES Code

O=C1/C(CN(CC)C/C1=C\C2=CC(OC)=C(OC)C(OC)=C2)=C/C3=CC(OC)=C(OC)C(OC)=C3

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>3 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

More Info

Product Data

Product Data

Instruction

View handling instruction

Biological target:

L48H37 is a potent and specific myeloid differentiation protein 2 (MD2) inhibitor and inhibits the interaction and signaling transduction of LPS-TLR4/MD2.

In vitro activity:

L48H37 up to 5 μM inhibited, without cytotoxicity, the motility, migration, and invasion of human osteosarcoma U2OS and MG-63 cells. Collectively, L48H37 represses the invasion and migration capabilities of U2OS and MG-63 cells by the suppression of uPA expression and the inhibition of JAK/STAT signaling. These results suggest that L48H37 may be a potential candidate for anti-metastatic treatment of human osteosarcoma. Reference: Molecules. 2020 Dec 23;26(1):30. https://pubmed.ncbi.nlm.nih.gov/33374783/

In vivo activity:

Administering L48H37 to mice bearing tumors derived from control or KMT2D-knockdown PDAC cells significantly decreased the tumor burden. Reference: World J Gastrointest Oncol. 2019 Aug 15;11(8):599-621. https://pubmed.ncbi.nlm.nih.gov/31435462/

	Solvent	mg/mL	mM
Solubility
	DMSO	50.0	103.40

Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 483.56 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Lu KH, Wu HH, Lin RC, Lin YC, Lu PW, Yang SF, Yang JS. Curcumin Analogue L48H37 Suppresses Human Osteosarcoma U2OS and MG-63 Cells' Migration and Invasion in Culture by Inhibition of uPA via the JAK/STAT Signaling Pathway. Molecules. 2020 Dec 23;26(1):30. doi: 10.3390/molecules26010030. PMID: 33374783; PMCID: PMC7795127. 2. Feng C, Xia Y, Zou P, Shen M, Hu J, Ying S, Pan J, Liu Z, Dai X, Zhuge W, Liang G, Ruan Y. Curcumin analog L48H37 induces apoptosis through ROS-mediated endoplasmic reticulum stress and STAT3 pathways in human lung cancer cells. Mol Carcinog. 2017 Jul;56(7):1765-1777. doi: 10.1002/mc.22633. Epub 2017 Mar 10. PMID: 28218464. 3. Li SS, Jiang WL, Xiao WQ, Li K, Zhang YF, Guo XY, Dai YQ, Zhao QY, Jiang MJ, Lu ZJ, Wan R. KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma. World J Gastrointest Oncol. 2019 Aug 15;11(8):599-621. doi: 10.4251/wjgo.v11.i8.599. PMID: 31435462; PMCID: PMC6700028. 4. Feng C, Xia Y, Zou P, Shen M, Hu J, Ying S, Pan J, Liu Z, Dai X, Zhuge W, Liang G, Ruan Y. Curcumin analog L48H37 induces apoptosis through ROS-mediated endoplasmic reticulum stress and STAT3 pathways in human lung cancer cells. Mol Carcinog. 2017 Jul;56(7):1765-1777. doi: 10.1002/mc.22633. Epub 2017 Mar 10. PMID: 28218464.

In vitro protocol:

In vivo protocol:

1. Li SS, Jiang WL, Xiao WQ, Li K, Zhang YF, Guo XY, Dai YQ, Zhao QY, Jiang MJ, Lu ZJ, Wan R. KMT2D deficiency enhances the anti-cancer activity of L48H37 in pancreatic ductal adenocarcinoma. World J Gastrointest Oncol. 2019 Aug 15;11(8):599-621. doi: 10.4251/wjgo.v11.i8.599. PMID: 31435462; PMCID: PMC6700028. 2. Feng C, Xia Y, Zou P, Shen M, Hu J, Ying S, Pan J, Liu Z, Dai X, Zhuge W, Liang G, Ruan Y. Curcumin analog L48H37 induces apoptosis through ROS-mediated endoplasmic reticulum stress and STAT3 pathways in human lung cancer cells. Mol Carcinog. 2017 Jul;56(7):1765-1777. doi: 10.1002/mc.22633. Epub 2017 Mar 10. PMID: 28218464.

1: Correction to "Curcumin Analog L48H37 Prevents Lipopolysaccharide-Induced TLR4 Signaling Pathway Activation and Sepsis via Targeting MD2". J Pharmacol Exp Ther. 2018 Apr;365(1):201. doi: 10.1124/jpet.117.222570err. PubMed PMID: 29559582. 2: Feng C, Xia Y, Zou P, Shen M, Hu J, Ying S, Pan J, Liu Z, Dai X, Zhuge W, Liang G, Ruan Y. Curcumin analog L48H37 induces apoptosis through ROS-mediated endoplasmic reticulum stress and STAT3 pathways in human lung cancer cells. Mol Carcinog. 2017 Jul;56(7):1765-1777. doi: 10.1002/mc.22633. Epub 2017 Mar 10. PubMed PMID: 28218464. 3: Wang Y, Shan X, Dai Y, Jiang L, Chen G, Zhang Y, Wang Z, Dong L, Wu J, Guo G, Liang G. Curcumin Analog L48H37 Prevents Lipopolysaccharide-Induced TLR4 Signaling Pathway Activation and Sepsis via Targeting MD2. J Pharmacol Exp Ther. 2015 Jun;353(3):539-50. doi: 10.1124/jpet.115.222570. Epub 2015 Apr 10. Erratum in: J Pharmacol Exp Ther. 2018 Apr;365(1):201. PubMed PMID: 25862641.