Synonym
SSE15206; SSE-15206; SSE 15206;
IUPAC/Chemical Name
4,5-Dihydro-3-phenyl-5-(3,4,5-trimethoxyphenyl)-1H-pyrazole-1-carbothioamide
InChi Key
GONIBFCARADPAC-UHFFFAOYSA-N
InChi Code
InChI=1S/C19H21N3O3S/c1-23-16-9-13(10-17(24-2)18(16)25-3)15-11-14(21-22(15)19(20)26)12-7-5-4-6-8-12/h4-10,15H,11H2,1-3H3,(H2,20,26)
SMILES Code
S=C(N1N=C(C2=CC=CC=C2)CC1C3=CC(OC)=C(OC)C(OC)=C3)N
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Biological target:
SSE15206 is a microtubule polymerization inhibitor (GI50 = 197 nM in HCT116 cells).
In vitro activity:
This study describes the synthesis and characterization of SSE15206. It can overcome drug resistance, including multidrug resistance, to MTAs. SSE15206 inhibits microtubule polymerization to induce apoptosis in various cancer cells. Importantly, it inhibits proliferation of paclitaxel-resistant cells, including MDR-1 expressing multidrug resistant cells, indicating that it is not affected by P-glycoprotein (Pgp).
Reference: Sci Rep. 2018; 8: 3305. https://pubmed.ncbi.nlm.nih.gov/29459693/
|
Solvent |
mg/mL |
mM |
comments |
| Solubility |
| DMSO |
112.0 |
301.52 |
|
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.
Preparing Stock Solutions
The following data is based on the
product
molecular weight
371.46
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
| 1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
| 5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
| 10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
| 50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
Formulation protocol:
1. Ma L, Lei J, Chen H, Huang R, Su T, Feng J, Sun Q. Structural insight into SSE15206 in complex with tubulin provides a rational design for pyrazolinethioamides as tubulin polymerization inhibitors. Future Med Chem. 2022 Jun;14(11):785-794. doi: 10.4155/fmc-2021-0124. Epub 2022 May 4. PMID: 35506429.
2. Manzoor S, Bilal A, Khan S, Ullah R, Iftikhar S, Emwas AH, Alazmi M, Gao X, Jawaid A, Saleem RSZ, Faisal A. Identification and characterization of SSE15206, a microtubule depolymerizing agent that overcomes multidrug resistance. Sci Rep. 2018 Feb 19;8(1):3305. doi: 10.1038/s41598-018-21642-0. Erratum in: Sci Rep. 2018 Apr 18;8(1):6427. PMID: 29459693; PMCID: PMC5818492.
In vitro protocol:
1. Ma L, Lei J, Chen H, Huang R, Su T, Feng J, Sun Q. Structural insight into SSE15206 in complex with tubulin provides a rational design for pyrazolinethioamides as tubulin polymerization inhibitors. Future Med Chem. 2022 Jun;14(11):785-794. doi: 10.4155/fmc-2021-0124. Epub 2022 May 4. PMID: 35506429.
2. Manzoor S, Bilal A, Khan S, Ullah R, Iftikhar S, Emwas AH, Alazmi M, Gao X, Jawaid A, Saleem RSZ, Faisal A. Identification and characterization of SSE15206, a microtubule depolymerizing agent that overcomes multidrug resistance. Sci Rep. 2018 Feb 19;8(1):3305. doi: 10.1038/s41598-018-21642-0. Erratum in: Sci Rep. 2018 Apr 18;8(1):6427. PMID: 29459693; PMCID: PMC5818492.
1: Manzoor S, Bilal A, Khan S, Ullah R, Iftikhar S, Emwas AH, Alazmi M, Gao X,
Jawaid A, Saleem RSZ, Faisal A. Author Correction: Identification and
characterization of SSE15206, a microtubule depolymerizing agent that overcomes
multidrug resistance. Sci Rep. 2018 Apr 18;8(1):6427. doi:
10.1038/s41598-018-24114-7. PubMed PMID: 29666376; PubMed Central PMCID:
PMC5904161.
2: Manzoor S, Bilal A, Khan S, Ullah R, Iftikhar S, Emwas AH, Alazmi M, Gao X,
Jawaid A, Saleem RSZ, Faisal A. Identification and characterization of SSE15206,
a microtubule depolymerizing agent that overcomes multidrug resistance. Sci Rep.
2018 Feb 19;8(1):3305. doi: 10.1038/s41598-018-21642-0. Erratum in: Sci Rep. 2018
Apr 18;8(1):6427. PubMed PMID: 29459693; PubMed Central PMCID: PMC5818492.
3: Ma L, Lei J, Chen H, Huang R, Su T, Feng J, Sun Q. Structural insight into SSE15206 in complex with tubulin provides a rational design for pyrazolinethioamides as tubulin polymerization inhibitors. Future Med Chem. 2022 Jun;14(11):785-794. doi: 10.4155/fmc-2021-0124. Epub 2022 May 4. PMID: 35506429.
