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MedKoo Cat#: 593048 | Name: Methylatropine bromide
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

Methylatropine is an antagonist of muscarinic acetylcholine receptors (IC50 = <0.1 nM in a radioligand binding assay using isolated porcine brain membranes) and a derivative of atropine. It reduces acetylcholine-induced decreases in blood pressure in rats when administered intravenously with an ED50 value of 5.5 µg/kg. Methylatropine reduces salivation, induces mydriasis, and increases heart rate in dogs.

Chemical Structure

Methylatropine bromide
Methylatropine bromide
CAS#2870-71-5 (bromide)

Theoretical Analysis

MedKoo Cat#: 593048

Name: Methylatropine bromide

CAS#: 2870-71-5 (bromide)

Chemical Formula: C18H26BrNO3

Exact Mass: 383.1096

Molecular Weight: 384.31

Elemental Analysis: C, 56.26; H, 6.82; Br, 20.79; N, 3.64; O, 12.49

Price and Availability

Size Price Availability Quantity
10mg USD 350.00 2 Weeks
25mg USD 650.00 2 Weeks
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Related CAS #
2870-71-5 (bromide) 52-88-0 (nitrate)
Synonym
Methylatropine Br; Methylatropine bromide; Atropine methylbromide; NSC 61810; NSC61810; NSC-61810;
IUPAC/Chemical Name
(1R,3s,5S)-3-((3-hydroxy-2-phenylpropanoyl)oxy)-8,8-dimethyl-8-azabicyclo[3.2.1]octan-8-ium bromide
InChi Key
XMLNCADGRIEXPK-VGGMPWLKSA-M
InChi Code
InChI=1S/C18H26NO3.BrH/c1-19(2)14-8-9-15(19)11-16(10-14)22-18(21)17(12-20)13-6-4-3-5-7-13;/h3-7,14-17,20H,8-12H2,1-2H3;1H/q+1;/p-1/t14-,15+,16-,17?;
SMILES Code
O=C(O[C@H]1C[C@H]2CC[C@@H](C1)[N+]2(C)C)C(C3=CC=CC=C3)CO.[Br-]
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Atropine methyl bromide, a muscarinic receptor (mAChR) antagonist, is a quaternary ammonium salt of atropine and a mydriatic for dilation of the pupil during ophthalmic examination.
In vitro activity:
The gene and the protein expression level results show that atropine is an effective molecule in reducing epithelial-mesenchymal transition (EMT) and colony formation induced by TGF-B or carboplatin in both the mesenchymal-like cell line MDA-MB-231 and the epithelial-like cell line T47D. Reference: Int J Mol Sci. 2022 Aug 30;23(17):9849. https://pubmed.ncbi.nlm.nih.gov/36077256/
In vivo activity:
When rats were injected with methylatropine i.c.v., the plasma glucose concentration increased, the insulin response reduced, and glucagon-like peptide-1 (7-36) amide (tGLP-1) was unchanged following an oral glucose load, compared with the controls. A transient increase in plasma insulin after selective hepatic vagotomy was inhibited by i.c.v. injection of methylatropine. Reference: J Auton Nerv Syst. 1996 Feb 5;57(1-2):43-8. https://pubmed.ncbi.nlm.nih.gov/8867084/
Solvent mg/mL mM comments
Solubility
Water 10.0 26.02
DMSO 150.0 390.31
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 384.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Ahmed EA, Alkuwayti MA, Ibrahim HM. Atropine Is a Suppressor of Epithelial-Mesenchymal Transition (EMT) That Reduces Stemness in Drug-Resistant Breast Cancer Cells. Int J Mol Sci. 2022 Aug 30;23(17):9849. doi: 10.3390/ijms23179849. PMID: 36077256; PMCID: PMC9456281. 2. Cristaldi M, Olivieri M, Pezzino S, Spampinato G, Lupo G, Anfuso CD, Rusciano D. Atropine Differentially Modulates ECM Production by Ocular Fibroblasts, and Its Ocular Surface Toxicity Is Blunted by Colostrum. Biomedicines. 2020 Apr 5;8(4):78. doi: 10.3390/biomedicines8040078. PMID: 32260532; PMCID: PMC7236597. 3. Ohnuma H, Yamatani K, Igarashi M, Sugiyama K, Manaka H, Tominaga M, Sasaki H. Intracerebroventricular injection of methylatropine suppresses insulin response to oral glucose load in rats. J Auton Nerv Syst. 1996 Feb 5;57(1-2):43-8. doi: 10.1016/0165-1838(95)00101-8. PMID: 8867084. 4. Smith RD, Grzelak ME, Coffin VL. Methylatropine blocks the central effects of cholinergic antagonists. Behav Pharmacol. 1994 Apr;5(2):167-175. doi: 10.1097/00008877-199404000-00008. PMID: 11224265.
In vitro protocol:
1. Ahmed EA, Alkuwayti MA, Ibrahim HM. Atropine Is a Suppressor of Epithelial-Mesenchymal Transition (EMT) That Reduces Stemness in Drug-Resistant Breast Cancer Cells. Int J Mol Sci. 2022 Aug 30;23(17):9849. doi: 10.3390/ijms23179849. PMID: 36077256; PMCID: PMC9456281. 2. Cristaldi M, Olivieri M, Pezzino S, Spampinato G, Lupo G, Anfuso CD, Rusciano D. Atropine Differentially Modulates ECM Production by Ocular Fibroblasts, and Its Ocular Surface Toxicity Is Blunted by Colostrum. Biomedicines. 2020 Apr 5;8(4):78. doi: 10.3390/biomedicines8040078. PMID: 32260532; PMCID: PMC7236597.
In vivo protocol:
1. Ohnuma H, Yamatani K, Igarashi M, Sugiyama K, Manaka H, Tominaga M, Sasaki H. Intracerebroventricular injection of methylatropine suppresses insulin response to oral glucose load in rats. J Auton Nerv Syst. 1996 Feb 5;57(1-2):43-8. doi: 10.1016/0165-1838(95)00101-8. PMID: 8867084. 2. Smith RD, Grzelak ME, Coffin VL. Methylatropine blocks the central effects of cholinergic antagonists. Behav Pharmacol. 1994 Apr;5(2):167-175. doi: 10.1097/00008877-199404000-00008. PMID: 11224265.
1: Silva LEV, Geraldini VR, de Oliveira BP, Silva CAA, Porta A, Fazan R. Comparison between spectral analysis and symbolic dynamics for heart rate variability analysis in the rat. Sci Rep. 2017 Aug 16;7(1):8428. doi: 10.1038/s41598-017-08888-w. PubMed PMID: 28814785; PubMed Central PMCID: PMC5559602. 2: Santos-Almeida FM, Domingos-Souza G, Meschiari CA, Fávaro LC, Becari C, Castania JA, Lopes A, Cunha TM, Moraes DJA, Cunha FQ, Ulloa L, Kanashiro A, Tezini GCSV, Salgado HC. Carotid sinus nerve electrical stimulation in conscious rats attenuates systemic inflammation via chemoreceptor activation. Sci Rep. 2017 Jul 24;7(1):6265. doi: 10.1038/s41598-017-06703-0. PubMed PMID: 28740186; PubMed Central PMCID: PMC5524712. 3: Magagna-Poveda A, Moretto JN, Scharfman HE. Increased gyrification and aberrant adult neurogenesis of the dentate gyrus in adult rats. Brain Struct Funct. 2017 Dec;222(9):4219-4237. doi: 10.1007/s00429-017-1457-4. Epub 2017 Jun 27. PubMed PMID: 28656372; PubMed Central PMCID: PMC5909844. 4: Hong LZ, Huang KF, Hung SW, Kuo LT. Chronic fluoxetine treatment enhances sympathetic activities associated with abnormality of baroreflex function in conscious normal rats. Eur J Pharmacol. 2017 Sep 15;811:164-170. doi: 10.1016/j.ejphar.2017.06.021. Epub 2017 Jun 16. PubMed PMID: 28629735. 5: Ko AR, Kang TC. TRPC6-mediated ERK1/2 phosphorylation prevents dentate granule cell degeneration via inhibiting mitochondrial elongation. Neuropharmacology. 2017 Jul 15;121:120-129. doi: 10.1016/j.neuropharm.2017.05.004. Epub 2017 May 4. PubMed PMID: 28479396. 6: Poterman M, Scheeren TWL, van der Velde MI, Buisman PL, Allaert S, Struys MMRF, Kalmar AF. Prophylactic atropine administration attenuates the negative haemodynamic effects of induction of anaesthesia with propofol and high-dose remifentanil: A randomised controlled trial. Eur J Anaesthesiol. 2017 Oct;34(10):695-701. doi: 10.1097/EJA.0000000000000639. PubMed PMID: 28403018. 7: Julien C, Oréa V, Quintin L, Piriou V, Barrès C. Renal sympathetic nerve activity and vascular reactivity to phenylephrine after lipopolysaccharide administration in conscious rats. Physiol Rep. 2017 Feb;5(4). pii: e13139. doi: 10.14814/phy2.13139. Epub 2017 Feb 27. PubMed PMID: 28242823; PubMed Central PMCID: PMC5328774. 8: Miyabara R, Berg K, Kraemer JF, Baltatu OC, Wessel N, Campos LA. Quantifying Effects of Pharmacological Blockers of Cardiac Autonomous Control Using Variability Parameters. Front Physiol. 2017 Jan 23;8:10. doi: 10.3389/fphys.2017.00010. eCollection 2017. PubMed PMID: 28167918; PubMed Central PMCID: PMC5253391. 9: Rojas A, Ganesh T, Walker A, Dingledine R. Ethylatropine Bromide as a Peripherally Restricted Muscarinic Antagonist. ACS Chem Neurosci. 2017 Apr 19;8(4):712-717. doi: 10.1021/acschemneuro.6b00334. Epub 2017 Jan 6. PubMed PMID: 28044440; PubMed Central PMCID: PMC5872150. 10: Alves-Santos TR, de Siqueira RJ, Duarte GP, Lahlou S. Cardiovascular Effects of the Essential Oil of Croton argyrophylloides in Normotensive Rats: Role of the Autonomic Nervous System. Evid Based Complement Alternat Med. 2016;2016:4106502. Epub 2016 Nov 13. PubMed PMID: 27956919; PubMed Central PMCID: PMC5124457. 11: Simões MR, Azevedo BF, Fiorim J, Jr Freire DD, Covre EP, Vassallo DV, Dos Santos L. Chronic mercury exposure impairs the sympathovagal control of the rat heart. Clin Exp Pharmacol Physiol. 2016 Nov;43(11):1038-1045. doi: 10.1111/1440-1681.12624. PubMed PMID: 27429172. 12: Klippel BF, Duemke LB, Leal MA, Friques AG, Dantas EM, Dalvi RF, Gava AL, Pereira TM, Andrade TU, Meyrelles SS, Campagnaro BP, Vasquez EC. Effects of Kefir on the Cardiac Autonomic Tones and Baroreflex Sensitivity in Spontaneously Hypertensive Rats. Front Physiol. 2016 Jun 7;7:211. doi: 10.3389/fphys.2016.00211. eCollection 2016. PubMed PMID: 27375490; PubMed Central PMCID: PMC4895057. 13: Simões MR, Preti SC, Azevedo BF, Fiorim J, Freire DD Jr, Covre EP, Vassallo DV, Dos Santos L. Low-level Chronic Lead Exposure Impairs Neural Control of Blood Pressure and Heart Rate in Rats. Cardiovasc Toxicol. 2017 Apr;17(2):190-199. doi: 10.1007/s12012-016-9374-y. PubMed PMID: 27272938. 14: Kanashiro A, Talbot J, Peres RS, Pinto LG, Bassi GS, Cunha TM, Cunha FQ. Neutrophil Recruitment and Articular Hyperalgesia in Antigen-Induced Arthritis are Modulated by the Cholinergic Anti-Inflammatory Pathway. Basic Clin Pharmacol Toxicol. 2016 Nov;119(5):453-457. doi: 10.1111/bcpt.12611. Epub 2016 May 20. PubMed PMID: 27098245. 15: Ribeiro-Filho HV, de Souza Silva CM, de Siqueira RJ, Lahlou S, dos Santos AA, Magalhães PJ. Biphasic cardiovascular and respiratory effects induced by β-citronellol. Eur J Pharmacol. 2016 Mar 15;775:96-105. doi: 10.1016/j.ejphar.2016.02.025. Epub 2016 Feb 9. PubMed PMID: 26872991. 16: Pinto TO, Lataro RM, Castania JA, Durand MT, Silva CA, Patel KP, Fazan R Jr, Salgado HC. Electrical stimulation of the aortic depressor nerve in conscious rats overcomes the attenuation of the baroreflex in chronic heart failure. Am J Physiol Regul Integr Comp Physiol. 2016 Apr 1;310(7):R612-8. doi: 10.1152/ajpregu.00392.2015. Epub 2016 Feb 3. PubMed PMID: 26843582. 17: Sayin H, Chapuis B, Chevalier P, Barrès C, Julien C. Assessment of cardiac autonomic tone in conscious rats. Auton Neurosci. 2016 Jan;194:26-31. doi: 10.1016/j.autneu.2015.12.007. Epub 2015 Dec 29. PubMed PMID: 26769133. 18: Gouveia MK, Miguel TT, Busnardo C, Scopinho AA, Corrêa FM, Nunes-de-Souza RL, Crestani CC. Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats. Neuropharmacology. 2016 Feb;101:379-88. doi: 10.1016/j.neuropharm.2015.10.018. Epub 2015 Oct 23. PubMed PMID: 26477570. 19: Blanco JH, Gastaldi AC, Gardim CB, Araujo JE, Simões MV, Oliveira LF, Carvalho EE, Souza HC. Chronic cholinergic stimulation promotes changes in cardiovascular autonomic control in spontaneously hypertensive rats. Auton Neurosci. 2015 Dec;193:97-103. doi: 10.1016/j.autneu.2015.09.002. Epub 2015 Oct 21. PubMed PMID: 26471065. 20: Maida KD, Souza HC. 6B.08: EFFECTS OF TREATMENT WITH ENALAPRIL OR LOSARTAN ASSOCIATED WITH AEROBIC PHYSICAL TRAINING ON CARDIOVASCULAR AUTONOMIC CONTROL IN SPONTANEOUSLY HYPERTENSIVE RATS (SHR). J Hypertens. 2015 Jun;33 Suppl 1:e78. doi: 10.1097/01.hjh.0000467563.03337.ec. PubMed PMID: 26102931.