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MedKoo Cat#: 563716 | Name: FAD Disodium Salt
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

FAD-Na2, also known as Flavin adenine dinucleotide, is a redox cofactor (electron carrier) by flavoproteins including succinate dehydrogenase (complex), α-ketoglutarate dehydrogenase, apoptosis-inducing factor 2 (AIF-M2, AMID), folate/FAD-dependent tRNA methyltransferases, and N-hydroxylating flavoprotein monooxygenases.

Chemical Structure

FAD Disodium Salt
FAD Disodium Salt
CAS#84366-81-4 (disodium)

Theoretical Analysis

MedKoo Cat#: 563716

Name: FAD Disodium Salt

CAS#: 84366-81-4 (disodium)

Chemical Formula: C27H31N9Na2O15P2

Exact Mass: 829.1210

Molecular Weight: 829.52

Elemental Analysis: C, 39.09; H, 3.77; N, 15.20; Na, 5.54; O, 28.93; P, 7.47

Price and Availability

Size Price Availability Quantity
100mg USD 350.00 2 Weeks
250mg USD 650.00 2 Weeks
500mg USD 950.00 2 Weeks
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Related CAS #
146-14-5 (free acid) 84366-81-4 (disodium) 1891059-93-0 (disodium hydrate)
Synonym
FAD Disodium Salt; FAD-Na2; FAD Na2; FADNa2; Flavin Adenine Dinucleotide Disodium Salt; Flavin adenine dinucleotide;
IUPAC/Chemical Name
Disodium [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl] [(2R,3S,4S)-5-(7,8-dimethyl-2,4-dioxobenzo[g]pteridin-10-yl)-2,3,4-trihydroxypentyl] phosphate
InChi Key
XLRHXNIVIZZOON-WFUPGROFSA-L
InChi Code
InChI=1S/C27H33N9O15P2.2Na/c1-10-3-12-13(4-11(10)2)35(24-18(32-12)25(42)34-27(43)33-24)5-14(37)19(39)15(38)6-48-52(44,45)51-53(46,47)49-7-16-20(40)21(41)26(50-16)36-9-31-17-22(28)29-8-30-23(17)36;;/h3-4,8-9,14-16,19-21,26,37-41H,5-7H2,1-2H3,(H,44,45)(H,46,47)(H2,28,29,30)(H,34,42,43);;/q;2*+1/p-2/t14-,15+,16+,19-,20+,21+,26+;;/m0../s1
SMILES Code
O=P(OP([O-])(OC[C@H]1O[C@@H](N2C=NC3=C(N)N=CN=C23)[C@H](O)[C@@H]1O)=O)([O-])OC[C@@H](O)[C@@H](O)[C@@H](O)CN4C5=CC(C)=C(C)C=C5N=C6C(NC(N=C46)=O)=O.[Na+].[Na+]
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>3 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.03.00
More Info
Product Data
Product Data
Instruction
View handling instruction
Biological target:
Flavin adenine dinucleotide (FAD) disodium salt is a redox cofactor involved in several important enzymatic reactions in metabolism.
In vitro activity:
In vitro experiments, FAD (Flavin adenine nucleotide) protected against the tBHP induced injury in HUVEC, by increasing the activity of SCAD, increasing the elimination of free fatty acid (FFA), scavenging ROS, reducing apoptotic rate, thereby improving endothelial cell function. Reference: Life Sci. 2020 Oct 1;258:118156. https://pubmed.ncbi.nlm.nih.gov/32735886/
In vivo activity:
In vivo experiments, FAD (Flavin adenine nucleotide) significantly decreased blood pressure and ameliorated vascular remodeling by increasing SCAD expression, Nitric Oxide (NO) production and reducing ROS production in rats. Reference: Life Sci. 2020 Oct 1;258:118156. https://pubmed.ncbi.nlm.nih.gov/32735886/
Solvent mg/mL mM
Solubility
DMSO 2.5 3.03
PBS (pH 7.2) 10.0 12.06
Water 33.3 40.18
Note: There can be variations in solubility for the same chemical from different vendors or different batches from the same vendor. The following factors can affect the solubility of the same chemical: solvent used for crystallization, residual solvent content, polymorphism, salt versus free form, degree of hydration, solvent temperature. Please use the solubility data as a reference only. Warming and sonication will facilitate dissolving. Still have questions? Please contact our Technical Support scientists.

Preparing Stock Solutions

The following data is based on the product molecular weight 829.52 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Zhong X, Ma Z, Su Y, Li Z, Liao Y, Pan X, Zang L, Zhou S. Flavin adenine dinucleotide ameliorates hypertensive vascular remodeling via activating short chain acyl-CoA dehydrogenase. Life Sci. 2020 Oct 1;258:118156. doi: 10.1016/j.lfs.2020.118156. Epub 2020 Jul 28. PMID: 32735886.
In vitro protocol:
1. Zhong X, Ma Z, Su Y, Li Z, Liao Y, Pan X, Zang L, Zhou S. Flavin adenine dinucleotide ameliorates hypertensive vascular remodeling via activating short chain acyl-CoA dehydrogenase. Life Sci. 2020 Oct 1;258:118156. doi: 10.1016/j.lfs.2020.118156. Epub 2020 Jul 28. PMID: 32735886.
In vivo protocol:
1. Zhong X, Ma Z, Su Y, Li Z, Liao Y, Pan X, Zang L, Zhou S. Flavin adenine dinucleotide ameliorates hypertensive vascular remodeling via activating short chain acyl-CoA dehydrogenase. Life Sci. 2020 Oct 1;258:118156. doi: 10.1016/j.lfs.2020.118156. Epub 2020 Jul 28. PMID: 32735886.
1: Voicescu M, Craciunescu O, Angelescu DG, Tatia R, Moldovan L. Spectroscopic, molecular dynamics simulation and biological studies of Flavin MonoNucleotide and Flavin Adenine Dinucleotide in biomimetic systems. Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 25;246:118997. doi: 10.1016/j.saa.2020.118997. Epub ahead of print. PMID: 33032115. 2: Zhong X, Ma Z, Su Y, Li Z, Liao Y, Pan X, Zang L, Zhou S. Flavin adenine dinucleotide ameliorates hypertensive vascular remodeling via activating short chain acyl-CoA dehydrogenase. Life Sci. 2020 Oct 1;258:118156. doi: 10.1016/j.lfs.2020.118156. Epub 2020 Jul 28. PMID: 32735886. 3: Lee H, Lee YS, Reginald SS, Baek S, Lee EM, Choi IG, Chang IS. Biosensing and electrochemical properties of flavin adenine dinucleotide (FAD)-Dependent glucose dehydrogenase (GDH) fused to a gold binding peptide. Biosens Bioelectron. 2020 Oct 1;165:112427. doi: 10.1016/j.bios.2020.112427. Epub 2020 Jul 4. PMID: 32729543. 4: Kong L, Wang Q, Deng Z, You D. Flavin Adenine Dinucleotide-Dependent Halogenase XanH and Engineering of Multifunctional Fusion Halogenases. Appl Environ Microbiol. 2020 Sep 1;86(18):e01225-20. doi: 10.1128/AEM.01225-20. PMID: 32651204; PMCID: PMC7480370. 5: Zhou G, Pan Q, Hu Z, Qiu J, Yu Z. Heterologous expression and characterization of flavinadenine dinucleotide synthetase from Candida famata for flavin adenine dinucleotide production. Protein Pept Lett. 2020 Jul 8. doi: 10.2174/0929866527666200708151327. Epub ahead of print. PMID: 32640951. 6: Berndt N, Kovács R, Rösner J, Wallach I, Dreier JP, Liotta A. Flavin Adenine Dinucleotide Fluorescence as an Early Marker of Mitochondrial Impairment During Brain Hypoxia. Int J Mol Sci. 2020 Jun 1;21(11):3977. doi: 10.3390/ijms21113977. PMID: 32492921; PMCID: PMC7312830. 7: Tsuruoka N, Soto SS, Tahar AB, Zebda A, Tsujimura S. Mediated electrochemical oxidation of glucose via poly(methylene green) grafted on the carbon surface catalyzed by flavin adenine dinucleotide-dependent glucose dehydrogenase. Colloids Surf B Biointerfaces. 2020 Apr 21;192:111065. doi: 10.1016/j.colsurfb.2020.111065. Epub ahead of print. PMID: 32361503. 8: Sjulstok E, Solov'yov IA. Structural Explanations of Flavin Adenine Dinucleotide Binding in Drosophila melanogaster Cryptochrome. J Phys Chem Lett. 2020 May 21;11(10):3866-3870. doi: 10.1021/acs.jpclett.0c00625. Epub 2020 May 4. PMID: 32330039. 9: Kieninger M, Ventura ON, Kottke T. Calculation of the Geometries and Infrared Spectra of the Stacked Cofactor Flavin Adenine Dinucleotide (FAD) as the Prerequisite for Studies of Light-Triggered Proton and Electron Transfer. Biomolecules. 2020 Apr 9;10(4):573. doi: 10.3390/biom10040573. PMID: 32283685; PMCID: PMC7226529. 10: Huang F, Zhang C, Liu Q, Zhao Y, Zhang Y, Qin Y, Li X, Li C, Zhou C, Jin N, Jiang C. Identification of amitriptyline HCl, flavin adenine dinucleotide, azacitidine and calcitriol as repurposing drugs for influenza A H5N1 virus- induced lung injury. PLoS Pathog. 2020 Mar 16;16(3):e1008341. doi: 10.1371/journal.ppat.1008341. PMID: 32176725; PMCID: PMC7075543. 11: Li MY, Wang YQ, Ying YL, Long YT. Revealing the transient conformations of a single flavin adenine dinucleotide using an aerolysin nanopore. Chem Sci. 2019 Sep 23;10(44):10400-10404. doi: 10.1039/c9sc03163d. PMID: 32110330; PMCID: PMC6988595. 12: Nguyen VL, Ah-Loo A, Fitzpatrick M. An automated workflow approach for the analysis of flavin adenine dinucleotide by HPLC with internal standard. Anal Biochem. 2020 Apr 1;594:113616. doi: 10.1016/j.ab.2020.113616. Epub 2020 Feb 7. PMID: 32035844. 13: Yen CA, Ruter DL, Turner CD, Pang S, Curran SP. Loss of flavin adenine dinucleotide (FAD) impairs sperm function and male reproductive advantage in C. elegans. Elife. 2020 Feb 5;9:e52899. doi: 10.7554/eLife.52899. PMID: 32022684; PMCID: PMC7032928. 14: Liu S, Diao N, Wang Z, Lu W, Tang YJ, Chen T. Modular Engineering of the Flavin Pathway in Escherichia coli for Improved Flavin Mononucleotide and Flavin Adenine Dinucleotide Production. J Agric Food Chem. 2019 Jun 12;67(23):6532-6540. doi: 10.1021/acs.jafc.9b02646. Epub 2019 Jun 3. PMID: 31099250. 15: Yamada K, Ito M, Kobayashi H, Hasegawa Y, Fukuda S, Yamaguchi S, Taketani T. Flavin adenine dinucleotide synthase deficiency due to FLAD1 mutation presenting as multiple acyl-CoA dehydrogenation deficiency-like disease: A case report. Brain Dev. 2019 Aug;41(7):638-642. doi: 10.1016/j.braindev.2019.04.002. Epub 2019 Apr 11. PMID: 30982706. 16: Ismail M, Schroeder L, Frese M, Kottke T, Hollmann F, Paul CE, Sewald N. Straightforward Regeneration of Reduced Flavin Adenine Dinucleotide Required for Enzymatic Tryptophan Halogenation. ACS Catal. 2019 Feb 1;9(2):1389-1395. doi: 10.1021/acscatal.8b04500. Epub 2019 Jan 4. PMID: 30775067; PMCID: PMC6369659. 17: Xu J, Li D, Lv J, Xu X, Wen B, Lin P, Liu F, Ji K, Shan J, Li H, Li W, Zhao Y, Zhao D, Pok JY, Yan C. ETFDH Mutations and Flavin Adenine Dinucleotide Homeostasis Disturbance Are Essential for Developing Riboflavin-Responsive Multiple Acyl-Coenzyme A Dehydrogenation Deficiency. Ann Neurol. 2018 Nov;84(5):659-673. doi: 10.1002/ana.25338. Epub 2018 Oct 19. PMID: 30232818. 18: Ishida K, Orihara K, Muguruma H, Iwasa H, Hiratsuka A, Tsuji K, Kishimoto T. Comparison of Direct and Mediated Electron Transfer in Electrodes with Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase. Anal Sci. 2018;34(7):783-787. doi: 10.2116/analsci.17P613. PMID: 29998959. 19: Giacomozzi L, Kjær C, Langeland Knudsen J, Andersen LH, Brøndsted Nielsen S, Stockett MH. Absorption and luminescence spectroscopy of mass-selected flavin adenine dinucleotide mono-anions. J Chem Phys. 2018 Jun 7;148(21):214309. doi: 10.1063/1.5024028. PMID: 29884035. 20: Antill LM, Woodward JR. Flavin Adenine Dinucleotide Photochemistry Is Magnetic Field Sensitive at Physiological pH. J Phys Chem Lett. 2018 May 17;9(10):2691-2696. doi: 10.1021/acs.jpclett.8b01088. Epub 2018 May 8. PMID: 29724094.

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