Synonym
Brilliant acid flavine 10J; Brilliant Acid Yellow 8G; Brilliant Sulfa Flavine; Brilliant sulfaflavine;
IUPAC/Chemical Name
1H-Benz(de)isoquinoline-5-sulfonic acid, 2,3-dihydro-6-amino-1,3-dioxo-2-(p-tolyl)-, monosodium salt
InChi Key
HYLDLLCHFLSKAG-UHFFFAOYSA-M
InChi Code
InChI=1S/C19H14N2O5S.Na/c1-10-5-7-11(8-6-10)21-18(22)13-4-2-3-12-16(13)14(19(21)23)9-15(17(12)20)27(24,25)26;/h2-9H,20H2,1H3,(H,24,25,26);/q;+1/p-1
SMILES Code
O=S(C1=C(N)C2=CC=CC(C(N(C3=CC=C(C)C=C3)C4=O)=O)=C2C4=C1)([O-])=O.[Na+]
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
Preparing Stock Solutions
The following data is based on the
product
molecular weight
404.37
Batch specific molecular weights may vary
from batch to batch
due to the degree of hydration, which will
affect the solvent
volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass |
1 mg |
5 mg |
10 mg |
1 mM |
1.15 mL |
5.76 mL |
11.51 mL |
5 mM |
0.23 mL |
1.15 mL |
2.3 mL |
10 mM |
0.12 mL |
0.58 mL |
1.15 mL |
50 mM |
0.02 mL |
0.12 mL |
0.23 mL |
1: Shapiro HM, Schildkraut ER, Curbelo R, Laird CW, Turner B, Hirschfeld T. Combined blood cell counting and classification with fluorochrome stains and flow instrumentation. J Histochem Cytochem. 1976 Jan;24(1):396-401. PubMed PMID: 56391.
2: Curtis SK, Cowden RR. Effects of preparation and fixation on three quantitative fluorescent cytochemical procedures. Histochemistry. 1980;68(1):29-38. PubMed PMID: 6157658.
3: Curtis SK, Cowden RR. Microfluorometric estimates of proteins associated with murine hepatocyte and thymocyte nuclei, residual structures, and nuclear matrix derivatives. Histochemistry. 1985;82(4):331-9. PubMed PMID: 4008276.
4: Freeman DA, Crissman HA. Evaluation of six fluorescent protein stains for use in flow microfluorometry. Stain Technol. 1975 Jul;50(4):279-84. PubMed PMID: 52920.
5: Aeby P, Schultze U, Braichotte D, Bundt M, Moser-Boroumand F, Wydler H, Fluhler H. Fluorescence imaging of tracer distributions in soil profiles. Environ Sci Technol. 2001 Feb 15;35(4):753-60. PubMed PMID: 11349288.
6: Oheim M, Naraghi M, Müller TH, Neher E. Two dye two wavelength excitation calcium imaging: results from bovine adrenal chromaffin cells. Cell Calcium. 1998 Jul;24(1):71-84. PubMed PMID: 9793690.
7: Cowden RR, Curtis SK. Microfluorometric investigations of chromatin structure. IV. Determination of total protein values in thymocyte and hepatocyte nuclei. Effects of extraction with 0.4 N H2SO4 and 0.35 M NaC1. Histochemistry. 1982;74(3):329-39. PubMed PMID: 7107322.