L-Dithiothreitol | CAS#16096-97-2 | chiral bidentate dithiol

MedKoo Cat#: 597039 | Name: L-Dithiothreitol

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Description:

WARNING: This product is for research use only, not for human or veterinary use.

L-Dithiothreitol is a chiral bidentate dithiol with two stereogenic centers, may be used in chiroptical response research. L-DDT is proposed as a stereoselective reducing agent for disulfide bridges in complex molecules.

Chemical Structure

L-Dithiothreitol

CAS#16096-97-2

Theoretical Analysis

MedKoo Cat#: 597039

Name: L-Dithiothreitol

CAS#: 16096-97-2

Chemical Formula: C4H10O2S2

Exact Mass: 154.0122

Molecular Weight: 154.24

Elemental Analysis: C, 31.15; H, 6.54; O, 20.75; S, 41.57

Price and Availability

Size	Price	Availability
250mg	USD 350.00	2 Weeks
500mg	USD 600.00	2 Weeks
1g	USD 950.00	2 Weeks

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Related CAS #

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Synonym

L-Dithiothreitol; L-Dtt;

IUPAC/Chemical Name

(2R,3R)-1,4-dimercaptobutane-2,3-diol

InChi Key

VHJLVAABSRFDPM-IMJSIDKUSA-N

InChi Code

InChI=1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1

SMILES Code

O[C@H]([C@@H](O)CS)CS

Appearance

Solid powder

Purity

>98% (or refer to the Certificate of Analysis)

Shipping Condition

Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition

Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility

Soluble in DMSO

Shelf Life

>2 years if stored properly

Drug Formulation

This drug may be formulated in DMSO

Stock Solution Storage

0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code

2934.99.9001

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Product Data

Product Data

Instruction

View handling instruction

Biological target:

L-Dithiothreitol is a chiral bidentate dithiol with two stereogenic centers, may be used in chiroptical response research.

In vitro activity:

D,L-dithiothreitol was used to reduce cellular homocystine to homocysteine. Reference: Biomed Chromatogr. 2007 Jan;21(1):107-12. https://pubmed.ncbi.nlm.nih.gov/17121413/

In vivo activity:

DTT (L-Dithiothreitol) treatment significantly inhibited cell proliferation and promoted apoptosis in rat hepatocytes. The levels of proteins related to ERS and endoplasmic reticulophagy, MERCs and the mitochondrial apoptosis pathway significantly increased in BRL-3A cells treated with DTT. DTT treatment decreased the ER-lysosome co-localization and enhanced the fluorescence intensity of Ca2+ in mitochondria. Reference: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Dec;38(12):1104-1110. https://pubmed.ncbi.nlm.nih.gov/36585233/

Preparing Stock Solutions

The following data is based on the product molecular weight 154.24 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:

1. Huang Y, Lu ZY, Brown KS, Whitehead AS, Blair IA. Quantification of intracellular homocysteine by stable isotope dilution liquid chromatography/tandem mass spectrometry. Biomed Chromatogr. 2007 Jan;21(1):107-12. doi: 10.1002/bmc.735. PMID: 17121413. 2. Sakai T, Daikai T, Monma H, Maeda H. Isolation from Nocardioides sp. strain CT16, purification, and characterization of a deoxycytidine deaminase extremely thermostable in the presence of D,L-dithiothreitol. Biosci Biotechnol Biochem. 2002 Aug;66(8):1646-51. doi: 10.1271/bbb.66.1646. PMID: 12353623. 3. Guo Y, Han B, Yang T, Chen Y, Yang Y, Liang C, Li J, Yang Q, Xie R. [Dithiothreitol promotes apoptosis in rat hepatocytes by inhibiting family with sequence similarity 134 member B (FAM134B)-mediated endoplasmic reticulophagy]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Dec;38(12):1104-1110. Chinese. PMID: 36585233. 4. Qi C, Li Y, Yu RQ, Zhou SL, Wang XG, Le GW, Jin QZ, Xiao H, Sun J. Composition and immuno-stimulatory properties of extracellular DNA from mouse gut flora. World J Gastroenterol. 2017 Nov 28;23(44):7830-7839. doi: 10.3748/wjg.v23.i44.7830. PMID: 29209124; PMCID: PMC5703912.

In vitro protocol:

In vivo protocol:

1. Guo Y, Han B, Yang T, Chen Y, Yang Y, Liang C, Li J, Yang Q, Xie R. [Dithiothreitol promotes apoptosis in rat hepatocytes by inhibiting family with sequence similarity 134 member B (FAM134B)-mediated endoplasmic reticulophagy]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2022 Dec;38(12):1104-1110. Chinese. PMID: 36585233. 2. Qi C, Li Y, Yu RQ, Zhou SL, Wang XG, Le GW, Jin QZ, Xiao H, Sun J. Composition and immuno-stimulatory properties of extracellular DNA from mouse gut flora. World J Gastroenterol. 2017 Nov 28;23(44):7830-7839. doi: 10.3748/wjg.v23.i44.7830. PMID: 29209124; PMCID: PMC5703912.

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