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MedKoo Cat#: 571623 | Name: DST Crosslinker
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

DST Crosslinker, or Disuccinimidyl tartrate, is a homobifunctional, cleavable crosslinking reagent (cleavable by oxidizing reagents). DST crosslinker is an excellent choice for applications in which crosslink cleavability is desired without disturbing protein disulfide bonds with reducing reagents.

Chemical Structure

DST Crosslinker
DST Crosslinker
CAS#62069-75-4

Theoretical Analysis

MedKoo Cat#: 571623

Name: DST Crosslinker

CAS#: 62069-75-4

Chemical Formula: C12H12N2O10

Exact Mass: 344.0492

Molecular Weight: 344.23

Elemental Analysis: C, 41.87; H, 3.51; N, 8.14; O, 46.48

Price and Availability

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250mg USD 650.00 2 Weeks
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Synonym
DST Crosslinker, DST Cross linker, DST Cross-linker; disuccinimidyl l-tartrate; Disuccinimidyl tartrate; Disuccinimidyl tartarate; disuccinimidyl-l-tartrate
IUPAC/Chemical Name
bis(2,5-dioxopyrrolidin-1-yl) (2R,3R)-2,3-dihydroxybutanedioate
InChi Key
NXVYSVARUKNFNF-UHFFFAOYSA-N
InChi Code
InChI=1S/C12H12N2O10/c15-5-1-2-6(16)13(5)23-11(21)9(19)10(20)12(22)24-14-7(17)3-4-8(14)18/h9-10,19-20H,1-4H2
SMILES Code
OC(C(C(ON1C(CCC1=O)=O)=O)O)C(ON2C(CCC2=O)=O)=O
Appearance
Solid powder
Purity
>95% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
DST protein crosslinker has a cis-diol that is cleaved with 15 mM Sodium meta-Periodate. The DST crosslinking reagent contains two N-hydroxysuccimide (NHS) ester groups and is reactive toward primary amines (amino groups), such as found on lysine and the N-terminus.
Product Data
Product Data
Instruction
View handling instruction
Biological target:
DST Crosslinker, or Disuccinimidyl tartrate, is a homobifunctional, cleavable crosslinking reagent (cleavable by oxidizing reagents).
In vitro activity:
A collagen casing was employed as an adherend to model soft tissue for the in vitro determination of bonding strength of tissue adhesives composed of various hydrophobically modified gelatins and disuccinimidyl tartrate. The adhesive composed of stearoyl-modified gelatin (7.4% stearoyl; 10Ste) and disuccinimidyl tartrate showed the highest bonding strength. Reference: Sci Technol Adv Mater. 2012 Nov 23;13(6):064212. https://pubmed.ncbi.nlm.nih.gov/27877539/
In vivo activity:
In vivo examination was performed using Japanese white rabbits, creating longitudinal tears on the avascular zone of meniscus and applying DST-HSA adhesive. Reference: Sci Technol Adv Mater. 2012 Dec 28;13(6):064219. https://pubmed.ncbi.nlm.nih.gov/27877546/

Preparing Stock Solutions

The following data is based on the product molecular weight 344.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
1. Matsuda M, Taguchi T. In vitro evaluation of tissue adhesives composed of hydrophobically modified gelatins and disuccinimidyl tartrate. Sci Technol Adv Mater. 2012 Nov 23;13(6):064212. doi: 10.1088/1468-6996/13/6/064212. PMID: 27877539; PMCID: PMC5099772. 2. Gardner MW, Brodbelt JS. Impact of proline and aspartic acid residues on the dissociation of intermolecularly crosslinked peptides. J Am Soc Mass Spectrom. 2008 Mar;19(3):344-57. doi: 10.1016/j.jasms.2007.11.006. Epub 2007 Nov 19. PMID: 18083526. 3. Inoue T, Taguchi T, Imade S, Kumahashi N, Uchio Y. Effectiveness and biocompatibility of a novel biological adhesive application for repair of meniscal tear on the avascular zone. Sci Technol Adv Mater. 2012 Dec 28;13(6):064219. doi: 10.1088/1468-6996/13/6/064219. PMID: 27877546; PMCID: PMC5099779.
In vitro protocol:
1. Matsuda M, Taguchi T. In vitro evaluation of tissue adhesives composed of hydrophobically modified gelatins and disuccinimidyl tartrate. Sci Technol Adv Mater. 2012 Nov 23;13(6):064212. doi: 10.1088/1468-6996/13/6/064212. PMID: 27877539; PMCID: PMC5099772. 2. Gardner MW, Brodbelt JS. Impact of proline and aspartic acid residues on the dissociation of intermolecularly crosslinked peptides. J Am Soc Mass Spectrom. 2008 Mar;19(3):344-57. doi: 10.1016/j.jasms.2007.11.006. Epub 2007 Nov 19. PMID: 18083526.
In vivo protocol:
1. Inoue T, Taguchi T, Imade S, Kumahashi N, Uchio Y. Effectiveness and biocompatibility of a novel biological adhesive application for repair of meniscal tear on the avascular zone. Sci Technol Adv Mater. 2012 Dec 28;13(6):064219. doi: 10.1088/1468-6996/13/6/064219. PMID: 27877546; PMCID: PMC5099779.
1: Aderibigbe BA, Varaprasad K, Sadiku ER, Ray SS, Mbianda XY, Fotsing MC, Owonubi SJ, Agwuncha SC. Kinetic release studies of nitrogen-containing bisphosphonate from gum acacia crosslinked hydrogels. Int J Biol Macromol. 2015 Feb;73:115-23. doi: 10.1016/j.ijbiomac.2014.10.064. Epub 2014 Nov 21. PubMed PMID: 25445681. 2: Gaucher SP, Hadi MZ, Young MM. Influence of crosslinker identity and position on gas-phase dissociation of Lys-Lys crosslinked peptides. J Am Soc Mass Spectrom. 2006 Mar;17(3):395-405. Epub 2006 Jan 27. PubMed PMID: 16443364. 3: Smith RJ, Capaldi RA, Muchmore D, Dahlquist F. Cross-linking of ubiquinone cytochrome c reductase (complex III) with periodate-cleavable bifunctional reagents. Biochemistry. 1978 Sep 5;17(18):3719-23. PubMed PMID: 212103. 4: Gardner MW, Brodbelt JS. Impact of proline and aspartic acid residues on the dissociation of intermolecularly crosslinked peptides. J Am Soc Mass Spectrom. 2008 Mar;19(3):344-57. Epub 2007 Nov 19. PubMed PMID: 18083526. 5: Finel M. Studies on the oligomeric state of isolated cytochrome oxidase using cross-linking reagents. Biochim Biophys Acta. 1987 Nov 19;894(2):174-9. PubMed PMID: 2823892. 6: Geisler N. Chemical crosslinking with disuccinimidyl tartrate defines the relative positions of the two antiparallel coiled coils of the desmin protofilament unit. FEBS Lett. 1993 May 24;323(1-2):63-7. PubMed PMID: 8495749.