MedKoo Cat#: 571622 | Name: DSSeb Crosslinker
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Description:

WARNING: This product is for research use only, not for human or veterinary use.

DSSeb Crosslinker, or Disuccinimidyl sebacate, is a homobifunctional crosslinking reagent which is cell membrane permeable. DSSeb Crosslinker is useful in protein crosslinking and making antibody drug conjugates (ADCs).

Chemical Structure

DSSeb Crosslinker
DSSeb Crosslinker
CAS#23024-29-5

Theoretical Analysis

MedKoo Cat#: 571622

Name: DSSeb Crosslinker

CAS#: 23024-29-5

Chemical Formula: C18H24N2O8

Exact Mass: 396.1533

Molecular Weight: 396.40

Elemental Analysis: Molecular Weight: 396.3960 Elemental Analysis: C, 54.54; H, 6.10; N, 7.07; O, 32.29

Price and Availability

Size Price Availability Quantity
1g USD 250.00 2 Weeks
2g USD 350.00 2 Weeks
5g USD 550.00 2 Weeks
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Related CAS #
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Synonym
DSSeb Crosslinker, DSSeb Cross linker, DSSeb Cross-linker; Disuccinimidyl Sebacate; Di(N-succinimidyl) sebacate; Di(N-succinimidyl) Sebacate
IUPAC/Chemical Name
bis(2,5-dioxopyrrolidin-1-yl) decanedioate
InChi Key
XPKIVYVYXKPGBP-UHFFFAOYSA-N
InChi Code
InChI=1S/C18H24N2O8/c21-13-9-10-14(22)19(13)27-17(25)7-5-3-1-2-4-6-8-18(26)28-20-15(23)11-12-16(20)24/h1-12H2
SMILES Code
O=C(ON1C(CCC1=O)=O)CCCCCCCCC(ON2C(CCC2=O)=O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
DSSeb crosslinking takes place via amine-reactive NHS esters at both ends of a 10-atom (14.1 Angstrom) spacer arm. DSSeb must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous crosslinking reaction.
Product Data
Biological target:
DSSeb Crosslinker, or Disuccinimidyl sebacate, is a homobifunctional crosslinking reagent which is cell membrane permeable.
In vitro activity:
TBD
In vivo activity:
TBD

Preparing Stock Solutions

The following data is based on the product molecular weight 396.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
TBD
In vitro protocol:
TBD
In vivo protocol:
TBD
1: Heck T, Faccio G, Richter M, Thöny-Meyer L. Enzyme-catalyzed protein crosslinking. Appl Microbiol Biotechnol. 2013 Jan;97(2):461-75. doi: 10.1007/s00253-012-4569-z. Epub 2012 Nov 25. Review. PubMed PMID: 23179622; PubMed Central PMCID: PMC3546294. 2: Reddy N, Reddy R, Jiang Q. Crosslinking biopolymers for biomedical applications. Trends Biotechnol. 2015 Jun;33(6):362-9. doi: 10.1016/j.tibtech.2015.03.008. Epub 2015 Apr 14. Review. PubMed PMID: 25887334. 3: Movahedi A, Lundin A, Kann N, Nydén M, Moth-Poulsen K. Cu(I) stabilizing crosslinked polyethyleneimine. Phys Chem Chem Phys. 2015 Jul 28;17(28):18327-36. doi: 10.1039/c5cp02198g. PubMed PMID: 26104912. 4: Reinitz SD, Currier BH, Levine RA, Van Citters DW. Crosslink density, oxidation and chain scission in retrieved, highly cross-linked UHMWPE tibial bearings. Biomaterials. 2014 May;35(15):4436-40. doi: 10.1016/j.biomaterials.2014.02.019. Epub 2014 Mar 1. PubMed PMID: 24589360. 5: Javadi MA, Feizi S. Sterile Keratitis following Collagen Crosslinking. J Ophthalmic Vis Res. 2014 Oct-Dec;9(4):510-3. doi: 10.4103/2008-322X.150832. PubMed PMID: 25709779; PubMed Central PMCID: PMC4329714.