MedKoo Cat#: 571617 | Name: BS2G free acid

Description:

WARNING: This product is for research use only, not for human or veterinary use.

BS2G Crosslinker, or Bis[Sulfosuccinimidyl] glutarate), is an amine-reactive, water soluble, homobifunctional protein crosslinker. The BS2G protein crosslinker's 5-atom (7.7 angstrom) spacer arm is non-cleavable and the molecule is not cell membrane permeable. The BS2G crosslinking reagent can be used to label cell surface proteins.

Chemical Structure

BS2G free acid
BS2G free acid
CAS#215597-83-4 (free acid)

Theoretical Analysis

MedKoo Cat#: 571617

Name: BS2G free acid

CAS#: 215597-83-4 (free acid)

Chemical Formula: C13H14N2O14S2

Exact Mass: 485.9886

Molecular Weight: 486.38

Elemental Analysis: C, 32.10; H, 2.90; N, 5.76; O, 46.05; S, 13.18

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Synonym
BS2G Crosslinker, BS2G Cross linker, BS2G Cross-linker
IUPAC/Chemical Name
Bis[Sulfosuccinimidyl] glutarate
InChi Key
OWSWOWLMQKUWTE-UHFFFAOYSA-N
InChi Code
InChI=1S/C13H14N2O14S2/c16-8-4-6(30(22,23)24)12(20)14(8)28-10(18)2-1-3-11(19)29-15-9(17)5-7(13(15)21)31(25,26)27/h6-7H,1-5H2,(H,22,23,24)(H,25,26,27)
SMILES Code
O=C(ON1C(C(S(=O)(O)=O)CC1=O)=O)CCCC(ON2C(C(S(=O)(O)=O)CC2=O)=O)=O
Appearance
Solid powder
Purity
>98% (or refer to the Certificate of Analysis)
Shipping Condition
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility
Soluble in DMSO
Shelf Life
>2 years if stored properly
Drug Formulation
This drug may be formulated in DMSO
Stock Solution Storage
0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code
2934.99.9001
More Info
The Sulfo-NHS esters of BS2G allow the crosslinking reaction to occur in aqueous solutions in near physiological conditions.

Preparing Stock Solutions

The following data is based on the product molecular weight 486.38 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
1: Kannan R, Santhoshkumar P, Mooney BP, Sharma KK. Identification of subunit-subunit interaction sites in αA-WT crystallin and mutant αA-G98R crystallin using isotope-labeled cross-linker and mass spectrometry. PLoS One. 2013 Jun 5;8(6):e65610. doi: 10.1371/journal.pone.0065610. Print 2013. PubMed PMID: 23755258; PubMed Central PMCID: PMC3673982.